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Mouse Anti-CBFB Antibody (1E6) (CBMAB-0194-YC)

Provided herein are mouse monoclonal antibodies against Human CBFB. The antibody clone 1E6 can be used for immunoassay techniques, such as IP, WB and MA.
See all CBFB antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E6
Antibody Isotype
IgG1
Application
IP, WB, MA

Basic Information

Immunogen
Recombinant protein domain
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CBFB
Introduction
CBFB (core-binding factor, beta subunit) is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis and osteogenesis. CBFB is a non-DNA binding regulatory subunit and allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters.
Entrez Gene ID
865
UniProt ID
Q13951
Alternative Names
Core-Binding Factor, Beta Subunit; Polyomavirus Enhancer-Binding Protein 2 Beta Subunit; SL3/AKV Core-Binding Factor Beta Subunit; SL3-3 Enhancer Factor 1 Subunit Beta; PEBP2-Beta; PEA2-Beta; CBF-Beta; Polyomavirus Enhancer Binding Protein 2, Beta Subunit; SL3-3 Enhancer Factor 1 Beta Subunit; PEBP2B
Function
Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.
Biological Process
Cell maturation Source: Ensembl
Definitive hemopoiesis Source: Ensembl
Lymphocyte differentiation Source: Ensembl
Myeloid cell differentiation Source: Ensembl
Negative regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Osteoblast differentiation Source: Ensembl
Positive regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Protein polyubiquitination Source: CACAO
Regulation of B cell receptor signaling pathway Source: Reactome
Regulation of bicellular tight junction assembly Source: Reactome
Regulation of cytokine-mediated signaling pathway Source: Reactome
Regulation of hematopoietic stem cell differentiation Source: Reactome
Regulation of intracellular estrogen receptor signaling pathway Source: Reactome
Regulation of keratinocyte differentiation Source: Reactome
Regulation of megakaryocyte differentiation Source: Reactome
Regulation of myeloid cell differentiation Source: Reactome
Regulation of regulatory T cell differentiation Source: Reactome
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
Regulation of Wnt signaling pathway Source: Reactome
Transcription by RNA polymerase II Source: ProtInc
Cellular Location
Nucleus
Involvement in disease
A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.

Li, G., Zhang, M., Huang, Y., Yang, J., Dong, L., Shi, H., ... & Li, J. (2021). The relationship between abnormal Core binding factor-β expression in human cartilage and osteoarthritis. BMC Musculoskeletal Disorders, 22(1), 1-13.

Kim, J. A., Shin, W. Y., Kim, J., Bang, H. I., Yoon, S. Y., Won, J. H., & Park, R. (2021). A Case of Acute Myeloid Leukemia With inv (16)(p13. 1q22); CBFB-MYH11 Presenting With Faggot Cells. Annals of Laboratory Medicine, 41(3), 333-335.

Li, F., Wu, H., Zou, G., Cang, D., & Shen, Y. (2021). Circular RNA_0062582 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via regulation of microRNA-145/CBFB axis. Bioengineered, 12(1), 1952-1963.

Guo, L., Chen, B., Zhang, G., Wang, Y., Cao, L., Ren, C., ... & Liao, N. (2020). The transcription factor CBFB mutations indicate an improved survival in HR+/HER2-breast cancer. Gene, 759, 144970.

van Bergen, M. G., Saes, J. L., Simons, A., Hebeda, K. M., Henskens, Y. M., Barteling, W., ... & van der Reijden, B. A. (2020). Platelet CD34 expression in a patient with a partial deletion of transcription factor subunit CBFB. American journal of hematology, 95(6), E136.

Shen, X., Liang, M., Chen, X., Pasha, M. A., D’Souza, S. S., Hidde, K., ... & Yang, Q. (2019). Cutting Edge: Core Binding Factor β Is Required for Group 2 Innate Lymphoid Cell Activation. The Journal of Immunology, 202(6), 1669-1673.

Lee-Thacker, S., Choi, Y., Taniuchi, I., Takarada, T., Yoneda, Y., Ko, C., & Jo, M. (2018). Core binding factor β expression in ovarian granulosa cells is essential for female fertility. Endocrinology, 159(5), 2094-2109.

Rapp, M., Lau, C. M., Adams, N. M., Weizman, O. E., O’Sullivan, T. E., Geary, C. D., & Sun, J. C. (2017). Core-binding factor β and Runx transcription factors promote adaptive natural killer cell responses. Science immunology, 2(18).

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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