Human Recombinant CBFB protein, His Tag (V2LY-0526-LY2612)

CBFB

Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.

Cell maturation Source: Ensembl
Definitive hemopoiesis Source: Ensembl
Lymphocyte differentiation Source: Ensembl
Myeloid cell differentiation Source: Ensembl
Negative regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Osteoblast differentiation Source: Ensembl
Positive regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Protein polyubiquitination Source: CACAO
Regulation of B cell receptor signaling pathway Source: Reactome
Regulation of bicellular tight junction assembly Source: Reactome
Regulation of cytokine-mediated signaling pathway Source: Reactome
Regulation of hematopoietic stem cell differentiation Source: Reactome
Regulation of intracellular estrogen receptor signaling pathway Source: Reactome
Regulation of keratinocyte differentiation Source: Reactome
Regulation of megakaryocyte differentiation Source: Reactome
Regulation of myeloid cell differentiation Source: Reactome
Regulation of regulatory T cell differentiation Source: Reactome
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
Regulation of Wnt signaling pathway Source: Reactome
Transcription by RNA polymerase II Source: ProtInc

Nucleus

A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.