Mouse Anti-CBLC Recombinant Antibody (3B4) (CBMAB-C5717-LY)

Name: Mouse Anti-CBLC Recombinant Antibody (3B4)
SKU: CBMAB-C5717-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3B4

Application

FC, WB

Immunogen

Human recombinant protein fragment corresponding to amino acids 244-474 of human CBLC produced in E.coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cas-Br-M (murine) ecotropic retroviral transforming sequence c

Introduction

This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Entrez Gene ID

23624

UniProt ID

Q9ULV8

Alternative Names

Cbl Proto-Oncogene C; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence C; Cas-Br-M (Murine) Ectropic Retroviral Transforming Sequence C; Cbl Proto-Oncogene C, E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase CBL-C; Signal Transduction Protein CBL-C; SH3-Binding Protein CBL-3; SH3-Binding Protein CBL-C; RING Finger Protein 57;

Function

Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.

Biological Process

Cell surface receptor signaling pathway Source: InterPro
Negative regulation of epidermal growth factor-activated receptor activity Source: BHF-UCL
Negative regulation of epidermal growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of MAP kinase activity Source: BHF-UCL
Protein ubiquitination Source: BHF-UCL
Signal transduction Source: GO_Central
Ubiquitin-dependent protein catabolic process Source: BHF-UCL

Cellular Location

Plasma membrane; Membrane raft

PTM

Phosphorylated on multiple tyrosine residues by SRC. Isoform 1, but not isoform 2, is phosphorylated on tyrosines by EGFR.
Autoubiquitinated when phosphorylated at Tyr-341, enhanced by SRC; suggesting proteasomal degradation.

Bataller, A., Guijarro, F., Caye, A., Strullu, M., Sterin, A., Molina, O., ... & Marschalek, R. (2021). KMT2A-CBL rearrangements in acute leukemias: clinical characteristics and genetic breakpoints. Blood Advances.

Hong, S. Y., Kao, Y. R., Lin, Y. P., Lee, M. H., Wu, J. Y., & Wu, C. W. (2020). CBLC functions as a novel therapeutic target to enhance the efficacy of paclitaxel on EGFR wild-type lung adenocarcinoma by downregulating AURKA.

Hong, S. Y., Kao, Y. R., Lee, T. C., & Wu, C. W. (2018). Upregulation of E3 ubiquitin ligase CBLC enhances EGFR dysregulation and signaling in lung adenocarcinoma. Cancer research, 78(17), 4984-4996.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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