Human Recombinant CBLC protein, GST Tag (V2LY-0526-LY2614)

Name: Human Recombinant CBLC protein, GST Tag
SKU: V2LY-0526-LY2614
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant CBLC protein, GST Tag consist of Amino Acid: 1-474 and predicts a molecular mass of 80.2 kDa.

Molecule Mass

80.2 kDa

Sequence

Amino Acid: 1-474

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile NaAC, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cas-Br-M (murine) ecotropic retroviral transforming sequence c

Function

Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.

Biological Process

Cell surface receptor signaling pathway Source: InterPro
Negative regulation of epidermal growth factor-activated receptor activity Source: BHF-UCL
Negative regulation of epidermal growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of MAP kinase activity Source: BHF-UCL
Protein ubiquitination Source: BHF-UCL
Signal transduction Source: GO_Central
Ubiquitin-dependent protein catabolic process Source: BHF-UCL

Cellular Location

Plasma membrane; Membrane raft

PTM

Phosphorylated on multiple tyrosine residues by SRC. Isoform 1, but not isoform 2, is phosphorylated on tyrosines by EGFR.
Autoubiquitinated when phosphorylated at Tyr-341, enhanced by SRC; suggesting proteasomal degradation.

Bataller, A., Guijarro, F., Caye, A., Strullu, M., Sterin, A., Molina, O., ... & Marschalek, R. (2021). KMT2A-CBL rearrangements in acute leukemias: clinical characteristics and genetic breakpoints. Blood Advances.

Hong, S. Y., Kao, Y. R., Lin, Y. P., Lee, M. H., Wu, J. Y., & Wu, C. W. (2020). CBLC functions as a novel therapeutic target to enhance the efficacy of paclitaxel on EGFR wild-type lung adenocarcinoma by downregulating AURKA.

Hong, S. Y., Kao, Y. R., Lee, T. C., & Wu, C. W. (2018). Upregulation of E3 ubiquitin ligase CBLC enhances EGFR dysregulation and signaling in lung adenocarcinoma. Cancer research, 78(17), 4984-4996.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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