Mouse Anti-CBX2 Recombinant Antibody (4C11B10) (CBMAB-C6717-LY)

Name: Mouse Anti-CBX2 Recombinant Antibody (4C11B10)
SKU: CBMAB-C6717-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4C11B10

Application

ELISA, WB, IHC-P, IF/C, FC

Immunogen

Purified recombinant fragment of human CBX2 (AA: 402-525) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:10,000
IF(ICC)	1:200-1:1,000
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CBX2

Introduction

This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2010]

Entrez Gene ID

Human	84733
Mouse	12416

UniProt ID

Human	Q14781
Mouse	P30658

Alternative Names

Chromobox 2; Cell Division Cycle Associated 6; Chromobox Homolog 2 (Pc Class Homolog, Drosophila); Chromobox Homolog 2 (Drosophila Pc Class); Pc Class Homolog (Drosophila); Chromobox Protein Homolog 2; Chromobox Homolog 2;

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:21282530).
PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:21282530).
Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity).
Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity).
Involved in sexual development, acting as activator of NR5A1 expression (PubMed:19361780).

Biological Process

Cell differentiation Source: UniProtKB-KW
Chromatin organization Source: UniProtKB-KW
Development of primary sexual characteristics Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB

Cellular Location

Nucleus; Chromosome. Localized in distinct foci on chromatin and in chromocenters. Localizes to the inactive X chromosome. Seems to be recruited to H3K27me3, H3K9ac and H3K3me2 sites on chromatin.

Involvement in disease

46,XY sex reversal 5 (SRXY5): A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.

Zhu, J., Luo, J. E., Chen, Y., & Wu, Q. (2021). Circ_0061140 knockdown inhibits tumorigenesis and improves PTX sensitivity by regulating miR-136/CBX2 axis in ovarian cancer. Journal of ovarian research, 14(1), 1-12.

Ueda, S., Kanda, M., Sato, Y., Baba, H., Nakamura, S., Sawaki, K., ... & Nomoto, S. (2020). Chromobox 2 expression predicts prognosis after curative resection of oesophageal squamous cell carcinoma. Cancer Genomics & Proteomics, 17(4), 391-400.

Wang, W., Li, X., Guan, C., Hu, Z., Zhao, Y., Li, W., & Jiang, X. (2020). LncRNA PCAT6 promotes the proliferation, migration and invasion of pancreatic ductal adenocarcinoma via regulating miR-185-5p/CBX2 axis. Pathology-Research and Practice, 216(9), 153074.

Tatavosian, R., Kent, S., Brown, K., Yao, T., Duc, H. N., Huynh, T. N., ... & Ren, X. (2019). Nuclear condensates of the Polycomb protein chromobox 2 (CBX2) assemble through phase separation. Journal of Biological Chemistry, 294(5), 1451-1463.

Wheeler, L. J., Watson, Z. L., Qamar, L., Yamamoto, T., Behbakht, K., & Bitler, B. G. (2019). Chromobox 2 protein identified as driver of anoikis-escape and disease progression in high grade serous ovarian cancer. Gynecologic Oncology, 154(1), e25-e26.

Mao, J., Tian, Y., Wang, C., Jiang, K., Li, R., Yao, Y., ... & Wang, L. (2019). CBX2 regulates proliferation and apoptosis via the phosphorylation of YAP in hepatocellular carcinoma. Journal of Cancer, 10(12), 2706.

Sun, D., Cao, X., & Wang, C. (2019). Polycomb chromobox Cbx2 enhances antiviral innate immunity by promoting Jmjd3-mediated demethylation of H3K27 at the Ifnb promoter. Protein & cell, 10(4), 285-294.

Wheeler, L. J., Watson, Z. L., Qamar, L., Yamamoto, T. M., Post, M. D., Berning, A. A., ... & Bitler, B. G. (2018). CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer. Oncogenesis, 7(11), 1-14.

Sproll, P., Eid, W., Gomes, C. R., Mendonca, B. B., Gomes, N. L., Costa, E. M. F., & Biason‐Lauber, A. (2018). Assembling the jigsaw puzzle: CBX 2 isoform 2 and its targets in disorders/differences of sex development. Molecular genetics & genomic medicine, 6(5), 785-795.

Chen, W. Y., Zhang, X. Y., Liu, T., Liu, Y., Zhao, Y. S., & Pang, D. (2017). Chromobox homolog 2 protein: A novel biomarker for predicting prognosis and Taxol sensitivity in patients with breast cancer. Oncology letters, 13(3), 1149-1156.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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