Mouse Anti-CCL3 Recombinant Antibody (CBFYM-0030) (CBMAB-M0035-FY)

Name: Mouse Anti-CCL3 Recombinant Antibody (CBFYM-0030)
SKU: CBMAB-M0035-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0030

Application

IF, WB, ELISA, IP, IHC-P

Immunogen

Amino acids 77-92 at the C-terminus of MIP-1α of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

C-C Motif Chemokine Ligand 3

Introduction

CCL3 is a Protein Coding gene. Diseases associated with CCL3 include Human Immunodeficiency Virus Type 1 and Chagas Disease. Among its related pathways are PEDF Induced Signaling and Toll-like receptor signaling pathway. Gene Ontology annotations related to this gene include identical protein binding and kinase activity. An important paralog of this gene is CCL3L3.

Entrez Gene ID

619514

UniProt ID

Q8HYQ3

Alternative Names

C-C Motif Chemokine Ligand 3; Small Inducible Cytokine A3 (Homologous To Mouse Mip-1a); Macrophage Inflammatory Protein 1-Alpha; Tonsillar Lymphocyte LD78 Alpha Protein; G0/G1 Switch Regulatory Protein 19-1; Chemokine (C-C Motif) Ligand 3; MIP-1-Alpha; PAT 464.1

Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Biological Process

Astrocyte cell migration Source: UniProtKB
Calcium ion transport Source: UniProtKB
Calcium-mediated signaling Source: UniProtKB
Cell activation Source: UniProtKB
Cell-cell signaling Source: UniProtKB
Cellular calcium ion homeostasis Source: UniProtKB
Cellular response to interferon-gamma Source: UniProtKB
Cellular response to interleukin-1 Source: UniProtKB
Cellular response to organic cyclic compound Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: UniProtKB
Cytokine-mediated signaling pathway Source: Reactome
Cytoskeleton organization Source: UniProtKB
Eosinophil chemotaxis Source: UniProtKB
Eosinophil degranulation Source: UniProtKB
Exocytosis Source: UniProtKB
G protein-coupled receptor signaling pathway Source: GO_Central
Granulocyte chemotaxis Source: UniProtKB
Inflammatory response Source: UniProtKB
Lipopolysaccharide-mediated signaling pathway Source: UniProtKB
Lymphocyte chemotaxis Source: UniProtKB
Macrophage chemotaxis Source: UniProtKB
MAPK cascade Source: UniProtKB
Monocyte chemotaxis Source: UniProtKB
Negative regulation by host of viral transcription Source: UniProtKB
Negative regulation of bone mineralization Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative regulation of osteoclast differentiation Source: UniProtKB
Neutrophil chemotaxis Source: UniProtKB
Osteoblast differentiation Source: UniProtKB
Positive regulation of calcium ion import Source: BHF-UCL
Positive regulation of calcium ion transport Source: UniProtKB
Positive regulation of calcium-mediated signaling Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of GTPase activity Source: GO_Central
Positive regulation of inflammatory response Source: UniProtKB
Positive regulation of interleukin-1 beta production Source: UniProtKB
Positive regulation of microglial cell activation Source: ARUK-UCL
Positive regulation of microglial cell migration Source: ARUK-UCL
Positive regulation of natural killer cell chemotaxis Source: UniProtKB
Positive regulation of neuron apoptotic process Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Positive regulation of tumor necrosis factor production Source: UniProtKB
Protein kinase B signaling Source: UniProtKB
Regulation of behavior Source: UniProtKB
Regulation of cell shape Source: UniProtKB
Regulation of sensory perception of pain Source: UniProtKB
Release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
Response to cholesterol Source: UniProtKB
Response to toxic substance Source: UniProtKB
Signaling Source: UniProtKB
T cell chemotaxis Source: UniProtKB

Cellular Location

Secreted

PTM

N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.

Dong, X., Shilin, M. B., Apalikova, O. V., Lukina, J. N., Golotin, V. A., Li, J., & Zhang, J. (2021). The Anti‐Infective Immune Mechanism of the CCL2 and CCL3 Chemokines in the Large Yellow Croaker (Larimichthys Crocea). Journal of Applied Ichthyology.

Yu, W., Ye, T., Ding, J., Huang, Y., Peng, Y., Xia, Q., & Cuntai, Z. (2021). miR-4456/CCL3/CCR5 pathway in the pathogenesis of tight junction impairment in chronic obstructive pulmonary disease. Frontiers in pharmacology, 12.

Luo, A., Meng, M., Wang, G., Han, R., Zhang, Y., Jing, X., ... & Zhao, X. (2020). Myeloid-derived suppressor cells recruited by chemokine (cc motif) ligand 3 promote the progression of breast cancer via phosphoinositide 3-kinase-protein kinase b-mammalian target of rapamycin signaling. Journal of breast cancer, 23(2), 141.

Ishida, Y., Kuninaka, Y., Nosaka, M., Kimura, A., Taruya, A., Furuta, M., ... & Kondo, T. (2020). Prevention of CaCl 2-induced aortic inflammation and subsequent aneurysm formation by the CCL3–CCR5 axis. Nature communications, 11(1), 1-15.

Luo, A., Meng, M., Wang, G., Han, R., Zhang, Y., Jing, X., ... & Zhao, X. (2020). Myeloid-Derived Suppressor Cells Recruited by Chemokine (CC Motif) Ligand 3 Promote the Progression of Breast Cancer via Phosphoinositide 3-Kinase-Protein Kinase B-Mammalian Target of Rapamycin Signaling. Journal of Breast Cancer, 23(2), 141-161.

Mir Seyed Nazari, P., Marosi, C., Moik, F., Riedl, J., Özer, Ö., Berghoff, A. S., ... & Ay, C. (2019). Low systemic levels of chemokine CC motif ligand 3 (CCL3) are associated with a high risk of venous thromboembolism in patients with glioma. Cancers, 11(12), 2020.

He, Z. C., Li, X. Y., Guo, Y. L., Ma, D., Fang, Q., Ren, L. L., ... & Wang, J. S. (2019). Heme oxygenase‐1 attenuates the inhibitory effect of bortezomib against the APRIL‐NF‐κB‐CCL3 signaling pathways in multiple myeloma cells: Corelated with bortezomib tolerance in multiple myeloma. Journal of cellular biochemistry, 120(5), 6972-6987.

Aust, V., Kress, E., Abraham, S., Schröder, N., Kipp, M., Stope, M. B., ... & Brandenburg, L. O. (2018). Lack of chemokine (CC motif) ligand 3 leads to decreased survival and reduced immune response after bacterial meningitis. Cytokine, 111, 246-254.

Salimi, V., Ramezani, A., Mirzaei, H., Tahamtan, A., Faghihloo, E., Rezaei, F., ... & Tavakoli-Yaraki, M. (2017). Evaluation of the expression level of 12/15 lipoxygenase and the related inflammatory factors (CCL5, CCL3) in respiratory syncytial virus infection in mice model. Microbial pathogenesis, 109, 209-213.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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