Mouse Anti-CCNA2 Recombinant Antibody (CBXC-2993) (CBMAB-C0309-CQ)

Name: Mouse Anti-CCNA2 Recombinant Antibody (CBXC-2993)
SKU: CBMAB-C0309-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-2993

Application

WB, IF

Immunogen

Human CCNA2 expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1-2 µg/ml
IF(ICC)	1-2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 1mg/mL BSA, 30% glycerol

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin A2

Introduction

CCNA2 (Cyclin A2) is a Protein Coding gene. Diseases associated with CCNA2 include Splenic Diffuse Red Pulp Small B-Cell Lymphoma and Valproate Embryopathy. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Signaling events mediated by PRL. Gene Ontology (GO) annotations related to this gene include protein kinase binding. An important paralog of this gene is CCNA1.

Entrez Gene ID

Human	890
Mouse	12428

UniProt ID

Human	P20248
Mouse	P51943

Alternative Names

Cyclin A2; Cyclin-A; CCN1; CCNA; Cyclin-A2; Cyclin A;

Function

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

Biological Process

Animal organ regeneration Source: Ensembl
Cell cycle G1/S phase transition Source: UniProtKB
Cell division Source: UniProtKB-KW
Cellular response to cocaine Source: Ensembl
Cellular response to estradiol stimulus Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cellular response to insulin-like growth factor stimulus Source: Ensembl
Cellular response to leptin stimulus Source: Ensembl
Cellular response to luteinizing hormone stimulus Source: Ensembl
Cellular response to nitric oxide Source: Ensembl
Cellular response to platelet-derived growth factor stimulus Source: Ensembl
Cochlea development Source: Ensembl
G2/M transition of mitotic cell cycle Source: UniProtKB
Histone phosphorylation Source: CAFA
Mitotic cell cycle phase transition Source: GO_Central
Positive regulation of fibroblast proliferation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: Ensembl
Protein deubiquitination Source: Reactome
Ras protein signal transduction Source: BHF-UCL
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of DNA replication Source: UniProtKB
Response to glucagon Source: Ensembl
Viral process Source: UniProtKB-KW

Cellular Location

Cytoplasm; Nucleus. Exclusively nuclear during interphase (PubMed:1312467). Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).

PTM

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Lu, Y., Su, F., Yang, H., Xiao, Y., Zhang, X., Su, H., ... & Ling, X. (2021). E2F1 transcriptionally regulates CCNA2 expression to promote triple negative breast cancer tumorigenicity. Cancer Biomarkers, (Preprint), 1-14.

Wang, H., Fu, L., Wei, D., Wang, B., Zhang, C., Zhu, T., ... & Yu, G. (2020). MiR-29c-3p suppresses the migration, invasion and cell cycle in esophageal carcinoma via CCNA2/p53 axis. Frontiers in bioengineering and biotechnology, 8, 75.

Lee, Y., Lee, C. E., Oh, S., Kim, H., Lee, J., Kim, S. B., & Kim, H. S. (2020). Pharmacogenomic analysis reveals CCNA2 as a predictive biomarker of sensitivity to polo-like kinase I inhibitor in gastric cancer. Cancers, 12(6), 1418.

Yang, R., Du, Y., Wang, L., Chen, Z., & Liu, X. (2020). Weighted gene co-expression network analysis identifies CCNA2 as a treatment target of prostate cancer through inhibiting cell cycle. Journal of Cancer, 11(5), 1203.

Yang, L., Zeng, W., Sun, H., Huang, F., Yang, C., Cai, X., ... & Yang, K. (2020). Bioinformatical analysis of gene expression omnibus database associates TAF7/CCNB1, TAF7/CCNA2, and GTF2E2/CDC20 pathways with glioblastoma development and prognosis. World neurosurgery, 138, e492-e514.

Li, X., Ma, X. L., Tian, F. J., Wu, F., Zhang, J., Zeng, W. H., ... & Zhang, Y. (2019). Downregulation of CCNA2 disturbs trophoblast migration, proliferation, and apoptosis during the pathogenesis of recurrent miscarriage. American Journal of Reproductive Immunology, 82(1), e13144.

Shekhar, R., Priyanka, P., Kumar, P., Ghosh, T., Khan, M. M., Nagarajan, P., & Saxena, S. (2019). The microRNAs miR-449a and miR-424 suppress osteosarcoma by targeting cyclin A2 expression. Journal of Biological Chemistry, 294(12), 4381-4400.

Alves, M. J., Goksel, M., Kaya, B., Mostafa, H., Gygli, P., Stephens, J., ... & Czeisler, C. M. (2019). CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus. The American journal of pathology, 189(2), 426-439.

Bayard, Q., Meunier, L., Peneau, C., Renault, V., Shinde, J., Nault, J. C., ... & Letouzé, E. (2018). Cyclin A2/E1 activation defines a hepatocellular carcinoma subclass with a rearrangement signature of replication stress. Nature communications, 9(1), 1-14.

Ruan, J. S., Zhou, H., Yang, L., Wang, L., Jiang, Z. S., & Wang, S. M. (2017). CCNA2 facilitates epithelial-to-mesenchymal transition via the integrin αvβ3 signaling in NSCLC. International journal of clinical and experimental pathology, 10(8), 8324.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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