Mouse Anti-CCND2 Recombinant Antibody (DCS-5) (CBMAB-C4836-CQ)

Name: Mouse Anti-CCND2 Recombinant Antibody (DCS-5)
SKU: CBMAB-C4836-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCND2 Recombinant Antibody (DCS-5) (CBMAB-C4836-CQ) in Immunoblot

Cell extracts were immunoblotted with indicated antibodies. β-Actin was used as a loading control. The amount of indicated proteins was quantified using ImageJ, and the relative values and GLI2FL/GLI2R ratio are shown below the image. ...View More

Kobayashi, T., Tanaka, K., Mashima, Y., Shoda, A., Tokuda, M., & Itoh, H. (2020). CEP164 deficiency causes hyperproliferation of pancreatic cancer cells. Frontiers in Cell and Developmental Biology, 8, 587691.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

DCS-5

Application

ELISA, IF, IP, WB, FC

Immunogen

Full length cyclin D2 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin D2

Introduction

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).

Entrez Gene ID

894

UniProt ID

P30279

Alternative Names

Cyclin D2; G1/S-Specific Cyclin D2; G1/S-Specific Cyclin-D2; KIAK0002; MPPH3;

Function

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Biological Process

Cell division Source: UniProtKB-KW
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of apoptotic process Source: BHF-UCL
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
Positive regulation of G1/S transition of mitotic cell cycle Source: BHF-UCL
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central

Cellular Location

Cytoplasm; Nucleus; Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
Isoform 2: Cytoplasm

Involvement in disease

Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3): A syndrome characterized by megalencephaly, ventriculomegaly that may lead to hydrocephalus, and polymicrogyria; polydactyly may also be seen. There is considerable phenotypic similarity between this disorder and the megalencephaly-capillary malformation syndrome.

PTM

Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Qian, Y., Wang, H., Zhang, Y., Wang, J. W., Fan, Y. C., Gao, S., & Wang, K. (2021). Hypermethylation of Cyclin D2 Predicts Poor Prognosis of Hepatitis B Virus-Associated Hepatocellular Carcinoma after Hepatectomy. The Tohoku Journal of Experimental Medicine, 254(3), 233-243.

Zhu, S., Zhang, J., Cui, Y., Tang, X., Gao, X., Li, D., & Jia, W. (2020). Long non-coding RNA HOXA11-AS upregulates Cyclin D2 to inhibit apoptosis and promote cell cycle progression in nephroblastoma by recruiting forkhead box P2. American journal of cancer research, 10(1), 284.

Qian, Y., Wang, J. W., Yu-Fang, X. D. Y., Fan, Y. C., Gao, S., & Wang, K. (2020). Measurement of Cyclin D2 (CCND2) Gene Promoter Methylation in Plasma and Peripheral Blood Mononuclear Cells and Alpha-Fetoprotein Levels in Patients with Hepatitis B Virus-Associated Hepatocellular Carcinoma. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 26, e927444-1.

Pandya, P., Jethva, M., Rubin, E., Birnbaum, R. Y., Braiman, A., & Isakov, N. (2019). PICOT binding to chromatin-associated EED negatively regulates cyclin D2 expression by increasing H3K27me3 at the CCND2 gene promoter. Cell death & disease, 10(10), 1-15.

Ding, Z. Y., Li, R., Zhang, Q. J., Wang, Y., Jiang, Y., Meng, Q. Y., ... & Wu, G. H. (2019). Prognostic role of cyclin D2/D3 in multiple human malignant neoplasms: A systematic review and meta‐analysis. Cancer medicine, 8(6), 2717-2729.

Huang, R., Hu, Z., Cao, Y., Li, H., Zhang, H., Su, W., ... & Jiang, L. (2019). MiR-652-3p inhibition enhances endothelial repair and reduces atherosclerosis by promoting Cyclin D2 expression. EBioMedicine, 40, 685-694.

Cameron, S. R., Nandi, S., Kahn, T. G., Barrasa, J. I., Stenberg, P., & Schwartz, Y. B. (2018). PTE, a novel module to target Polycomb Repressive Complex 1 to the human cyclin D2 (CCND2) oncogene. Journal of Biological Chemistry, 293(37), 14342-14358.

Koochakpoor, G., Mirmiran, P., Daneshpour, M. S., Hosseini-Esfahani, F., Sedaghati-Khayat, B., Hosseini, S. A., & Azizi, F. (2018). Dietary factors influence the association of cyclin D2 polymorphism rs11063069 with the risk of metabolic syndrome. Nutrition Research, 52, 48-56.

Zhao, Z., Yang, F., Liu, Y., Fu, K., & Jing, S. (2018). MicroRNA‑409‑3p suppresses cell proliferation and cell cycle progression by targeting cyclin D2 in papillary thyroid carcinoma. Oncology letters, 16(4), 5237-5242.

Wang, Y., Xue, J., Kuang, H., Zhou, X., Liao, L., & Yin, F. (2017). microRNA-1297 inhibits the growth and metastasis of colorectal cancer by suppressing cyclin D2 expression. DNA and cell biology, 36(11), 991-999.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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