Mouse Anti-CCND3 Recombinant Antibody (CBFYC-1040) (CBMAB-C1097-FY)

Name: Mouse Anti-CCND3 Recombinant Antibody (CBFYC-1040)
SKU: CBMAB-C1097-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCND3 Recombinant Antibody (CBFYC-1040) (CBMAB-C1097-FY) in WB

Western blot with SPAG5, cyclin A, cyclin B, cyclin D1, and cyclin D3 of whole-cell lysate of MCF-10A cells stably overexpressing either pcMV6 or SPAG5 (left panel) and MCF-10A cells stably overexpressing SPAG5 transfected with siGFP or siSPAG5 (right panel). ...View More

Canu, V., Donzelli, S., Sacconi, A., Lo Sardo, F., Pulito, C., Bossel, N., ... & Blandino, G. (2021). Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation. Cell Death & Differentiation, 28(5), 1493-1511.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1040

Application

WB, IF, IP, WB

Immunogen

Full length cyclin D3 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 127-292

More Infomation

Target

Full Name

Cyclin D3

Introduction

Amplification and overexpression of the CCND3 gene was detected in the glioblastoma cell line CCF‐STTG1, as well as in one primary glioblastoma and in the sarcomatous component of one gliosarcoma.

Entrez Gene ID

Human	896
Mouse	12445
Rat	25193
Dog	608847

UniProt ID

Human	P30281
Mouse	P30282
Rat	P48961
Dog	F1PC17

Alternative Names

Cyclin D3

Function

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Biological Process

Cell division Source: UniProtKB-KW
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
Positive regulation of G1/S transition of mitotic cell cycle Source: GO_Central
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of cell population proliferation Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of insulin receptor signaling pathway Source: Ensembl
Signal transduction Source: Ensembl
T cell proliferation Source: Ensembl

Cellular Location

Cytoplasm; Nucleus; Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.

PTM

Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Smith, C. C., Viny, A. D., Massi, E., Kandoth, C., Socci, N. D., Rapaport, F., ... & Shah, N. P. (2021). Recurrent Mutations in Cyclin D3 Confer Clinical Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Clinical Cancer Research.

Pae, J., Ersching, J., Castro, T. B., Schips, M., Mesin, L., Allon, S. J., ... & Victora, G. D. (2021). Cyclin D3 drives inertial cell cycling in dark zone germinal center B cells. Journal of Experimental Medicine, 218(4).

Wu, H., Wang, C., Liu, Y., Yang, C., Liang, X., Zhang, X., & Li, X. (2020). miR‑138‑5p suppresses glioblastoma cell viability and leads to cell cycle arrest by targeting cyclin D3. Oncology Letters, 20(5), 1-1.

Ramezani-Rad, P., Chen, C., Zhu, Z., & Rickert, R. C. (2020). Cyclin D3 governs clonal expansion of dark zone germinal center B cells. Cell reports, 33(7), 108403.

Martín-Garcia, D., Navarro, A., Valdés-Mas, R., Clot, G., Gutiérrez-Abril, J., Prieto, M., ... & Beà, S. (2019). CCND2 and CCND3 hijack immunoglobulin light-chain enhancers in cyclin D1− mantle cell lymphoma. Blood, The Journal of the American Society of Hematology, 133(9), 940-951.

Kang, H., Heo, S., Shin, J. J., Ji, E., Tak, H., Ahn, S., ... & Kim, W. (2019). A miR‐194/PTBP1/CCND3 axis regulates tumor growth in human hepatocellular carcinoma. The Journal of pathology, 249(3), 395-408.

Ding, Z. Y., Li, R., Zhang, Q. J., Wang, Y., Jiang, Y., Meng, Q. Y., ... & Wu, G. H. (2019). Prognostic role of cyclin D2/D3 in multiple human malignant neoplasms: A systematic review and meta‐analysis. Cancer medicine, 8(6), 2717-2729.

Demin, D. E., Bogolyubova, A. V., Zlenko, D. V., Uvarova, A. N., Deikin, A. V., Putlyaeva, L. V., ... & Schwartz, A. M. (2018). The novel short isoform of securin stimulates the expression of cyclin D3 and angiogenesis factors VEGFA and FGF2, but does not affect the expression of MYC transcription factor. Molecular Biology, 52(3), 436-445.

Yu, X., Zhang, Y., Cavazos, D., Ma, X., Zhao, Z., Du, L., & Pertsemlidis, A. (2018). miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer. Cell death & disease, 9(2), 1-12.

Curiel-Olmo, S., Mondéjar, R., Almaraz, C., Mollejo, M., Cereceda, L., Marès, R., ... & Martínez, N. (2017). Splenic diffuse red pulp small B-cell lymphoma displays increased expression of cyclin D3 and recurrent CCND3 mutations. Blood, The Journal of the American Society of Hematology, 129(8), 1042-1045.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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