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Mouse Anti-CCNF Recombinant Antibody (CBYY-C1125) (CBMAB-C2562-YY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C1125
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 774-798 at the C-terminus of cyclin F of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin F
Entrez Gene ID
899
UniProt ID
P41002
Alternative Names
NEP; SFE; CD10; CALLA; CMT2T; SCA43
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.
Biological Process
Cell division Source: UniProtKB-KW
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of centrosome duplication Source: UniProtKB
Placenta development Source: Ensembl
Post-translational protein modification Source: Reactome
Protein polyubiquitination Source: Reactome
Protein ubiquitination Source: UniProtKB
Re-entry into mitotic cell cycle Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Centriole; Nucleus. Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus.
PTM
Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence.
Phosphorylated just before cells enter into mitosis.
More Infomation

Zelong, Y., Han, Y., Ting, G., Yifei, W., Kun, H., Haoran, H., & Yong, C. (2021). Increased expression of Cyclin F in liver cancer predicts poor prognosis: A study based on TCGA database. Medicine, 100(31).

Rayner, S. L., Cheng, F., Hogan, A. L., Grima, N., Yang, S., Ke, Y. D., ... & Lee, A. (2021). ALS/FTD-causing mutation in cyclin F causes the dysregulation of SFPQ. Human Molecular Genetics, 30(11), 971-984.

Klimaszewska-Wiśniewska, A., Buchholz, K., Neska-Długosz, I., Durślewicz, J., Grzanka, D., Zabrzyński, J., ... & Gagat, M. (2021). Expression of Genomic Instability-Related Molecules: Cyclin F, RRM2 and SPDL1 and Their Prognostic Significance in Pancreatic Adenocarcinoma. Cancers, 13(4), 859.

Burdova, K., Yang, H., Faedda, R., Hume, S., Chauhan, J., Ebner, D., ... & D'Angiolella, V. (2019). E2F1 proteolysis via SCF‐cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition. The EMBO journal, 38(20), e101443.

Yu, Y., Nakagawa, T., Morohoshi, A., Nakagawa, M., Ishida, N., Suzuki, N., ... & Nakayama, K. (2019). Pathogenic mutations in the ALS gene CCNF cause cytoplasmic mislocalization of Cyclin F and elevated VCP ATPase activity. Human molecular genetics, 28(20), 3486-3497.

Sun, Y., Fu, L., Xue, F., Li, Y., Xu, H., & Chen, J. (2019). Digital gene expression profiling and validation study highlight Cyclin F as an important regulator for sperm motility of chickens. Poultry science, 98(10), 5118-5126.

Clijsters, L., Hoencamp, C., Calis, J. J., Marzio, A., Handgraaf, S. M., Cuitino, M. C., ... & Pagano, M. (2019). Cyclin F controls cell-cycle transcriptional outputs by directing the degradation of the three activator E2Fs. Molecular cell, 74(6), 1264-1277.

Gagat, M., Krajewski, A., Grzanka, D., & Grzanka, A. (2018). Potential role of cyclin F mRNA expression in the survival of skin melanoma patients: Comprehensive analysis of the pathways altered due to cyclin F upregulation. Oncology reports, 40(1), 123-144.

Galper, J., Rayner, S. L., Hogan, A. L., Fifita, J. A., Lee, A., Chung, R. S., ... & Yang, S. (2017). Cyclin F: a component of an E3 ubiquitin ligase complex with roles in neurodegeneration and cancer. The international journal of biochemistry & cell biology, 89, 216-220.

Deshmukh, R. S., & Das, S. (2017). Cyclin F controls glioma progression by regulation of IDH1-R132H expression. Annals of Oncology, 28, x174.

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For research use only. Not intended for any clinical use.

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