Rabbit Anti-CCNF Recombinant Antibody (D9K2U) (CBMAB-C1470-CN)

Name: Rabbit Anti-CCNF Recombinant Antibody (D9K2U)
SKU: CBMAB-C1470-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D9K2U

Application

Immunogen

Synthetic peptide corresponding to residues surrounding Pro665 of human Cyclin F protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

More Infomation

Target

Full Name

Cyclin F

Introduction

CCNF (Cyclin F) is a Protein Coding gene. Diseases associated with CCNF include Amyotrophic Lateral Sclerosis 1 and Necrotizing Fasciitis. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation.

Entrez Gene ID

899

UniProt ID

P41002

Alternative Names

Cyclin F; F-Box Only Protein 1; FBXO1; FBX1; G2/Mitotic-Specific Cyclin-F; Cyclin-F;

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.

Biological Process

Cell division Source: UniProtKB-KW
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of centrosome duplication Source: UniProtKB
Placenta development Source: Ensembl
Post-translational protein modification Source: Reactome
Protein polyubiquitination Source: Reactome
Protein ubiquitination Source: UniProtKB
Re-entry into mitotic cell cycle Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB

Cellular Location

Centriole; Nucleus. Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus.

PTM

Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence.
Phosphorylated just before cells enter into mitosis.

Zelong, Y., Han, Y., Ting, G., Yifei, W., Kun, H., Haoran, H., & Yong, C. (2021). Increased expression of Cyclin F in liver cancer predicts poor prognosis: A study based on TCGA database. Medicine, 100(31).

Rayner, S. L., Cheng, F., Hogan, A. L., Grima, N., Yang, S., Ke, Y. D., ... & Lee, A. (2021). ALS/FTD-causing mutation in cyclin F causes the dysregulation of SFPQ. Human Molecular Genetics, 30(11), 971-984.

Klimaszewska-Wiśniewska, A., Buchholz, K., Neska-Długosz, I., Durślewicz, J., Grzanka, D., Zabrzyński, J., ... & Gagat, M. (2021). Expression of Genomic Instability-Related Molecules: Cyclin F, RRM2 and SPDL1 and Their Prognostic Significance in Pancreatic Adenocarcinoma. Cancers, 13(4), 859.

Burdova, K., Yang, H., Faedda, R., Hume, S., Chauhan, J., Ebner, D., ... & D'Angiolella, V. (2019). E2F1 proteolysis via SCF‐cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition. The EMBO journal, 38(20), e101443.

Yu, Y., Nakagawa, T., Morohoshi, A., Nakagawa, M., Ishida, N., Suzuki, N., ... & Nakayama, K. (2019). Pathogenic mutations in the ALS gene CCNF cause cytoplasmic mislocalization of Cyclin F and elevated VCP ATPase activity. Human molecular genetics, 28(20), 3486-3497.

Sun, Y., Fu, L., Xue, F., Li, Y., Xu, H., & Chen, J. (2019). Digital gene expression profiling and validation study highlight Cyclin F as an important regulator for sperm motility of chickens. Poultry science, 98(10), 5118-5126.

Clijsters, L., Hoencamp, C., Calis, J. J., Marzio, A., Handgraaf, S. M., Cuitino, M. C., ... & Pagano, M. (2019). Cyclin F controls cell-cycle transcriptional outputs by directing the degradation of the three activator E2Fs. Molecular cell, 74(6), 1264-1277.

Gagat, M., Krajewski, A., Grzanka, D., & Grzanka, A. (2018). Potential role of cyclin F mRNA expression in the survival of skin melanoma patients: Comprehensive analysis of the pathways altered due to cyclin F upregulation. Oncology reports, 40(1), 123-144.

Galper, J., Rayner, S. L., Hogan, A. L., Fifita, J. A., Lee, A., Chung, R. S., ... & Yang, S. (2017). Cyclin F: a component of an E3 ubiquitin ligase complex with roles in neurodegeneration and cancer. The international journal of biochemistry & cell biology, 89, 216-220.

Deshmukh, R. S., & Das, S. (2017). Cyclin F controls glioma progression by regulation of IDH1-R132H expression. Annals of Oncology, 28, x174.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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