Mouse Anti-CCNK Recombinant Antibody (3B7-1B9) (CBMAB-C5749-LY)

Name: Mouse Anti-CCNK Recombinant Antibody (3B7-1B9)
SKU: CBMAB-C5749-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3B7-1B9

Application

ELISA, WB

Immunogen

Full length recombinant corresponding to aa1-354 from CCNK with GST tag.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cyclin K

Introduction

CCNK (Cyclin K) is a Protein Coding gene. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. Gene Ontology (GO) annotations related to this gene include protein kinase binding and RNA polymerase II carboxy-terminal domain kinase activity. An important paralog of this gene is CCNT2.

Entrez Gene ID

8812

UniProt ID

O75909

Alternative Names

Cyclin K; CPR4; Cyclin-K;

Function

Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: MGI
Negative regulation by host of viral genome replication Source: CACAO
Positive regulation of DNA-templated transcription, elongation Source: GO_Central
Positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues Source: CACAO
Positive regulation of transcription by RNA polymerase II Source: Reactome
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Regulation of transcription by RNA polymerase II Source: GO_Central
snRNA transcription by RNA polymerase II Source: Reactome
Transcription by RNA polymerase II Source: Reactome
Transcription elongation from RNA polymerase II promoter Source: Reactome

Cellular Location

Nucleus

Involvement in disease

Intellectual developmental disorder with hypertelorism and distinctive facies (IDDHDF): An autosomal dominant neurodevelopmental disorder characterized by developmental delay and intellectual disability, language defects, and distinctive facial dysmorphism including high hairline, hypertelorism, thin eyebrows, dysmorphic ears, broad nasal bridge and tip, and narrow jaw.

Dieter, S. M., Siegl, C., Codó, P. L., Huerta, M., Ostermann-Parucha, A. L., Schulz, E., ... & Glimm, H. (2021). Degradation of CCNK/CDK12 is a druggable vulnerability of colorectal cancer. Cell reports, 36(3), 109394.

Niu, T., Li, K., Jiang, L., Zhou, Z., Hong, J., Chen, X., ... & Zhu, C. L. (2021). Noncovalent CDK12/13 dual inhibitors-based PROTACs degrade CDK12-Cyclin K complex and induce synthetic lethality with PARP inhibitor. European Journal of Medicinal Chemistry, 114012.

Lv, L., Chen, P., Cao, L., Li, Y., Zeng, Z., Cui, Y., ... & Han, T. (2020). Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation. Elife, 9, e59994.

Słabicki, M., Kozicka, Z., Petzold, G., Li, Y. D., Manojkumar, M., Bunker, R. D., ... & Ebert, B. L. (2020). The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K. Nature, 585(7824), 293-297.

Żuryń, A., Krajewski, A., Klimaszewska‑Wiśniewska, A., Grzanka, A., & Grzanka, D. (2019). Expression of cyclin B1, D1 and K in non‑small cell lung cancer H1299 cells following treatment with sulforaphane. Oncology reports, 41(2), 1313-1323.

Hoshii, T., Cifani, P., Feng, Z., Huang, C. H., Koche, R., Chen, C. W., ... & Armstrong, S. A. (2018). A non-catalytic function of SETD1A regulates cyclin K and the DNA damage response. Cell, 172(5), 1007-1021.

Fan, Y., Yin, W., Hu, B., Kline, A. D., Zhang, V. W., Liang, D., ... & Yu, Y. (2018). De novo mutations of CCNK cause a syndromic neurodevelopmental disorder with distinctive facial dysmorphism. The American Journal of Human Genetics, 103(3), 448-455.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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