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Mouse Anti-CCNK Recombinant Antibody (3B7-1B9) (CBMAB-C5749-LY)

This product is antibody recognizes CCNK. The antibody 3B7-1B9 immunoassay techniques such as: ELISA, WB.
See all CCNK antibodies
Published Data
Fig. 1 The bait-proteins for immunoprecipitations (IP) were detected with their respective molecular weights in the lysates.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3B7-1B9
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
Full length recombinant corresponding to aa1-354 from CCNK (AAH15935) with GST tag. MW of the GST tag alone is 26kD
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
cyclin K
Introduction
CCNK (Cyclin K) is a Protein Coding gene. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. Gene Ontology (GO) annotations related to this gene include protein kinase binding and RNA polymerase II carboxy-terminal domain kinase activity. An important paralog of this gene is CCNT2.
Entrez Gene ID
8812
UniProt ID
O75909
Alternative Names
Cyclin K; CPR4; Cyclin-K;
Function
Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: MGI
Negative regulation by host of viral genome replication Source: CACAO
Positive regulation of DNA-templated transcription, elongation Source: GO_Central
Positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues Source: CACAO
Positive regulation of transcription by RNA polymerase II Source: Reactome
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Regulation of transcription by RNA polymerase II Source: GO_Central
snRNA transcription by RNA polymerase II Source: Reactome
Transcription by RNA polymerase II Source: Reactome
Transcription elongation from RNA polymerase II promoter Source: Reactome
Cellular Location
Nucleus
Involvement in disease
Intellectual developmental disorder with hypertelorism and distinctive facies (IDDHDF): An autosomal dominant neurodevelopmental disorder characterized by developmental delay and intellectual disability, language defects, and distinctive facial dysmorphism including high hairline, hypertelorism, thin eyebrows, dysmorphic ears, broad nasal bridge and tip, and narrow jaw.

Dieter, S. M., Siegl, C., Codó, P. L., Huerta, M., Ostermann-Parucha, A. L., Schulz, E., ... & Glimm, H. (2021). Degradation of CCNK/CDK12 is a druggable vulnerability of colorectal cancer. Cell reports, 36(3), 109394.

Niu, T., Li, K., Jiang, L., Zhou, Z., Hong, J., Chen, X., ... & Zhu, C. L. (2021). Noncovalent CDK12/13 dual inhibitors-based PROTACs degrade CDK12-Cyclin K complex and induce synthetic lethality with PARP inhibitor. European Journal of Medicinal Chemistry, 114012.

Lv, L., Chen, P., Cao, L., Li, Y., Zeng, Z., Cui, Y., ... & Han, T. (2020). Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation. Elife, 9, e59994.

Słabicki, M., Kozicka, Z., Petzold, G., Li, Y. D., Manojkumar, M., Bunker, R. D., ... & Ebert, B. L. (2020). The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K. Nature, 585(7824), 293-297.

Żuryń, A., Krajewski, A., Klimaszewska‑Wiśniewska, A., Grzanka, A., & Grzanka, D. (2019). Expression of cyclin B1, D1 and K in non‑small cell lung cancer H1299 cells following treatment with sulforaphane. Oncology reports, 41(2), 1313-1323.

Hoshii, T., Cifani, P., Feng, Z., Huang, C. H., Koche, R., Chen, C. W., ... & Armstrong, S. A. (2018). A non-catalytic function of SETD1A regulates cyclin K and the DNA damage response. Cell, 172(5), 1007-1021.

Fan, Y., Yin, W., Hu, B., Kline, A. D., Zhang, V. W., Liang, D., ... & Yu, Y. (2018). De novo mutations of CCNK cause a syndromic neurodevelopmental disorder with distinctive facial dysmorphism. The American Journal of Human Genetics, 103(3), 448-455.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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