Mouse Anti-CCNO Recombinant Antibody (CBXC-1782) (CBMAB-C4673-CQ)

Name: Mouse Anti-CCNO Recombinant Antibody (CBXC-1782)
SKU: CBMAB-C4673-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1782

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-267 mapping at the N-terminal of cyclin O of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin O

Introduction

CCNO (Cyclin O) is a Protein Coding gene. Diseases associated with CCNO include Ciliary Dyskinesia, Primary, 29 and Primary Ciliary Dyskinesia. Gene Ontology (GO) annotations related to this gene include protein kinase binding and uracil DNA N-glycosylase activity. An important paralog of this gene is CCNB1.

Entrez Gene ID

10309

UniProt ID

P22674

Alternative Names

Cyclin O; Cyclin U; Cyclin Domain Containing; Cyclin-O; CILD29; UDG2; CCNU;

Function

Specifically required for generation of multiciliated cells, possibly by promoting a cell cycle state compatible with centriole amplification and maturation. Acts downstream of MCIDAS to promote mother centriole amplification and maturation in preparation for apical docking.

Biological Process

Cell division Source: UniProtKB-KW
Cilium assembly Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Mitotic cell cycle phase transition Source: GO_Central
Multi-ciliated epithelial cell differentiation Source: UniProtKB
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Response to drug Source: Ensembl

Cellular Location

Cytoplasm; Nucleolus. Localizes to the apical part of cytoplasm.

Involvement in disease

Ciliary dyskinesia, primary, 29 (CILD29): The disease is caused by variants affecting the gene represented in this entry. Marked reduction of cilia in multiciliate cells due to defective mother centriole generation and placement. Remaining cilia correctly express axonemal motor proteins, are motile and do not show beating defects. Defects are probably caused by a strong reduction in the number of multiple motile cilia covering the cell surface in respiratory epithelial cells (PubMed:24747639). A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia. CILD29 patients do not exhibit situs inversus, a congenital abnormality in which visceral organs are opposite to their normal positions (situs solitus) due to lateral transposition.

Xu, X., Dong, L., Ma, C., Xu, X., Wu, H., Wang, J., ... & Wei, Z. (2021). Establishment of an induced pluripotent stem cell line from a patient with primary ciliary dyskinesia carrying biallelic mutations in CCNO. Stem Cell Research, 53, 102372.

Ma, C., Wu, H., Zhu, D., Wang, Y., Shen, Q., Cheng, H., ... & Xu, X. (2021). Bi‐allelic mutations in MCIDA S and CCNO cause human infertility associated with abnormal gamete transport. Clinical Genetics, 100(6), 731-742.

Wang, J., & Chen, S. (2020). RACK1 promotes miR-302b/c/d-3p expression and inhibits CCNO expression to induce cell apoptosis in cervical squamous cell carcinoma. Cancer Cell International, 20(1), 1-12.

Dahlmann, J., Sahabian, A., Drick, N., Haase, A., Göhring, G., Lachmann, N., ... & Olmer, R. (2020). Generation of two hiPSC lines (MHHi016-A, MHHi016-B) from a primary ciliary dyskinesia patient carrying a homozygous 5 bp duplication (c. 248_252dup (p. Gly85Cysfs* 11)) in exon 1 of the CCNO gene. Stem Cell Research, 46, 101850.

Li, L., Cao, Y., Zhou, H., Li, Y., He, B., Zhou, X., ... & Ye, L. (2018). Knockdown of CCNO decreases the tumorigenicity of gastric cancer by inducing apoptosis. OncoTargets and therapy, 11, 7471.

Núnez-Ollé, M., Jung, C., Terré, B., Balsiger, N. A., Plata, C., Roset, R., ... & Gil-Gómez, G. (2017). Constitutive Cyclin O deficiency results in penetrant hydrocephalus, impaired growth and infertility. Oncotarget, 8(59), 99261.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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