Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0403) (CBMAB-C0444-FY)

Name: Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0403)
SKU: CBMAB-C0444-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0403) (CBMAB-C0444-FY) in IF and WB

CCR4 in intestinal was determined by WB was analyzed, compared with control. Immunofluorescence co-location assay was performed, incubation with anti-CCL3 (CY3-Tyramide), anti-CCR4(FITC), anti-E-Cad (a calcium-dependent transmembrane protein distributed in various epithelial cells) and DAPI as indicated (Scale bars: 50 μm). ...View More

Yuan, X., Xiong, Z., Liu, W., Li, Y., Li, H., Zhang, X., ... & Song, Z. (2022). Novel therapeutic targeting of CCL3-CCR4 axis mediated apoptotic intesitnal injury in necrotizing enterocolitis. Frontiers in Immunology, 13, 859398.

Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0403) (CBMAB-C0444-FY) in IF

Representative images of TUNEL (FITC) stained sections from control, NEC, NEC+anti-CCR4 and NEC+PDTC groups (Scale bar =100 μm). ...View More

Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0403) (CBMAB-C0444-FY) in WB

IEC-6 cells were treatment with LPS or LPS+anti-CCR4, then the cells were harvest at different time (0,1,3,6h) for signal molecule phosphorylation detection by WB. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0403

Application

WB, IP, IF, ELISA, FC

Immunogen

CKR-4 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

C-C Motif Chemokine Receptor 4

Introduction

CCR4 (C-C Motif Chemokine Receptor 4) is a Protein Coding gene. Diseases associated with CCR4 include Cutaneous T Cell Lymphoma and Mycosis Fungoides. Among its related pathways are Akt Signaling and Peptide ligand-binding receptors. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and C-C chemokine receptor activity. An important paralog of this gene is CCR5.

Entrez Gene ID

1233

UniProt ID

P51679

Alternative Names

C-C Motif Chemokine Receptor 4; Chemokine (C-C Motif) Receptor 4; C-C CKR-4; CC-CKR-4; CMKBR4; CCR-4; K5-5; C-C Chemokine Receptor Type 4

Function

High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.

Biological Process

Calcium-mediated signaling Source: GO_Central
Cell chemotaxis Source: GO_Central
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
Homeostasis of number of cells Source: Ensembl
Immune response Source: GO_Central
Inflammatory response Source: ProtInc
Neuron migration Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of positive chemotaxis Source: Ensembl
Response to antibiotic Source: Ensembl
Response to bacterium Source: Ensembl
Response to radiation Source: Ensembl
Tolerance induction Source: Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 1-39
Helical: 40-67
Cytoplasmic: 68-77
Helical: 78-98
Extracellular: 99-111
Helical: 112-133
Cytoplasmic: 134-150
Helical: 151-175
Extracellular: 176-206
Helical: 207-226
Cytoplasmic: 227-242
Helical: 243-267
Extracellular: 268-284
Helical: 285-308
Cytoplasmic: 309-360

PTM

In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.

Xu, H., Lin, S., & Huang, H. (2021). Involvement of increased expression of chemokine C–C motif chemokine 22 (CCL22)/CC chemokine receptor 4 (CCR4) in the inflammatory injury and cartilage degradation of chondrocytes. Cytotechnology, 73(5), 715-726.

Lim, H. D., Lane, J. R., Canals, M., & Stone, M. J. (2021). Systematic Assessment of Chemokine Signaling at Chemokine Receptors CCR4, CCR7 and CCR10. International Journal of Molecular Sciences, 22(8), 4232.

Tang, N., Zhang, Y. Y., Cheng, J. H., Zhao, Z. B., & Fan, Y. (2021). Cross-talk between CXC chemokine ligand 10-CXC chemokine receptor 3 axis and CC chemokine ligand 17-CC chemokine receptor 4 axis in the pathogenesis of oral lichen planus. Hua xi kou Qiang yi xue za zhi= Huaxi Kouqiang Yixue Zazhi= West China Journal of Stomatology, 39(4), 405-412.

Dong, Y., Gao, S., Zhang, X., Kou, J., Liu, J., Ye, T., & Shen, H. (2021). CCL17 and CCL22 induce CCR4 receptor expression and promote cytokine-induced killer cells migration. Anti-cancer Drugs.

Dabbaghmanesh, M. H., Rezaei, B., Haghshenas, M. R., Montazeri-Najafabady, N., Mohammadian Amiri, R., & Erfani, N. (2021). CCR4 1014C/T and CCL22 16C/A Genetic Variations in the Iranian Patients with Thyroid Cancer. Middle East Journal of Cancer.

Ohuchi, K., Fujimura, T., Lyu, C., Amagai, R., Muto, Y., & Aiba, S. (2020). Serum CCL22 levels decreased in parallel with disease activity in CCR4‐positive mycosis fungoides treated with mogamulizumab. Dermatologic Therapy, 33(6), e14099.

Zhong, Y., Lin, Z., Lu, J., Lin, X., Xu, W., Wang, N., ... & Lin, S. (2019). CCL2-CCL5/CCR4 contributed to radiation-induced epithelial-mesenchymal transition of HPAEpiC cells via the ERK signaling pathways. American journal of translational research, 11(2), 733.

Wang, L., Zhang, M., Zhu, Y., Zhang, X., Yang, Y., & Wang, C. (2019). CCR4 expression is associated with poor prognosis in patients with early stage (pN0) oral tongue cancer. Journal of Oral and Maxillofacial Surgery, 77(2), 426-432.

Sato, T., & Yamano, Y. (2018). Targeting human T‐lymphotropic virus type 1‐infected cells with an anti‐C‐C chemokine receptor 4 antibody in T‐lymphotropic virus type 1‐associated myelopathy. Clinical and Experimental Neuroimmunology, 9(3), 153-154.

Klein, A., Sagi-Assif, O., Meshel, T., Telerman, A., Izraely, S., Ben-Menachem, S., ... & Witz, I. P. (2017). CCR4 is a determinant of melanoma brain metastasis. Oncotarget, 8(19), 31079.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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