Human Recombinant CD194-VLP protein, Flag & His-Strep Tag (V2LY-0526-LY2638)

Name: Human Recombinant CD194-VLP protein, Flag & His-Strep Tag
SKU: V2LY-0526-LY2638
Rating: 5 (1 reviews)

See all CCR4 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

Flag & His-Strep Tag

Protein Construction

This product is Human Recombinant CD194-VLP protein, Flag & His-Strep Tag consist of Amino Acid: 1-320 and predicts a molecular mass of 41.02 kDa.

Molecule Mass

41.02 kDa

Sequence

Amino Acid: 1-320

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -70°C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

C-C Motif Chemokine Receptor 4

Function

High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.

Biological Process

Calcium-mediated signaling Source: GO_Central
Cell chemotaxis Source: GO_Central
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
Homeostasis of number of cells Source: Ensembl
Immune response Source: GO_Central
Inflammatory response Source: ProtInc
Neuron migration Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of positive chemotaxis Source: Ensembl
Response to antibiotic Source: Ensembl
Response to bacterium Source: Ensembl
Response to radiation Source: Ensembl
Tolerance induction Source: Ensembl

Cellular Location

Cell membrane

Topology

Extracellular: 1-39
Helical: 40-67
Cytoplasmic: 68-77
Helical: 78-98
Extracellular: 99-111
Helical: 112-133
Cytoplasmic: 134-150
Helical: 151-175
Extracellular: 176-206
Helical: 207-226
Cytoplasmic: 227-242
Helical: 243-267
Extracellular: 268-284
Helical: 285-308
Cytoplasmic: 309-360

PTM

In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.

Xu, H., Lin, S., & Huang, H. (2021). Involvement of increased expression of chemokine C–C motif chemokine 22 (CCL22)/CC chemokine receptor 4 (CCR4) in the inflammatory injury and cartilage degradation of chondrocytes. Cytotechnology, 73(5), 715-726.

Lim, H. D., Lane, J. R., Canals, M., & Stone, M. J. (2021). Systematic Assessment of Chemokine Signaling at Chemokine Receptors CCR4, CCR7 and CCR10. International Journal of Molecular Sciences, 22(8), 4232.

Tang, N., Zhang, Y. Y., Cheng, J. H., Zhao, Z. B., & Fan, Y. (2021). Cross-talk between CXC chemokine ligand 10-CXC chemokine receptor 3 axis and CC chemokine ligand 17-CC chemokine receptor 4 axis in the pathogenesis of oral lichen planus. Hua xi kou Qiang yi xue za zhi= Huaxi Kouqiang Yixue Zazhi= West China Journal of Stomatology, 39(4), 405-412.

Dong, Y., Gao, S., Zhang, X., Kou, J., Liu, J., Ye, T., & Shen, H. (2021). CCL17 and CCL22 induce CCR4 receptor expression and promote cytokine-induced killer cells migration. Anti-cancer Drugs.

Dabbaghmanesh, M. H., Rezaei, B., Haghshenas, M. R., Montazeri-Najafabady, N., Mohammadian Amiri, R., & Erfani, N. (2021). CCR4 1014C/T and CCL22 16C/A Genetic Variations in the Iranian Patients with Thyroid Cancer. Middle East Journal of Cancer.

Ohuchi, K., Fujimura, T., Lyu, C., Amagai, R., Muto, Y., & Aiba, S. (2020). Serum CCL22 levels decreased in parallel with disease activity in CCR4‐positive mycosis fungoides treated with mogamulizumab. Dermatologic Therapy, 33(6), e14099.

Zhong, Y., Lin, Z., Lu, J., Lin, X., Xu, W., Wang, N., ... & Lin, S. (2019). CCL2-CCL5/CCR4 contributed to radiation-induced epithelial-mesenchymal transition of HPAEpiC cells via the ERK signaling pathways. American journal of translational research, 11(2), 733.

Wang, L., Zhang, M., Zhu, Y., Zhang, X., Yang, Y., & Wang, C. (2019). CCR4 expression is associated with poor prognosis in patients with early stage (pN0) oral tongue cancer. Journal of Oral and Maxillofacial Surgery, 77(2), 426-432.

Sato, T., & Yamano, Y. (2018). Targeting human T‐lymphotropic virus type 1‐infected cells with an anti‐C‐C chemokine receptor 4 antibody in T‐lymphotropic virus type 1‐associated myelopathy. Clinical and Experimental Neuroimmunology, 9(3), 153-154.

Klein, A., Sagi-Assif, O., Meshel, T., Telerman, A., Izraely, S., Ben-Menachem, S., ... & Witz, I. P. (2017). CCR4 is a determinant of melanoma brain metastasis. Oncotarget, 8(19), 31079.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant CD194-VLP protein, Flag & His-Strep Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CCR4 ELISA Kit (CAT#: V2LY-0626-LY580)

Related Products

Rabbit Anti-CCR4 Recombinant Antibody (EG520) (CAT#: CBMAB-EN593-LY)

Mouse Anti-CCR4 Recombinant Antibody (9G12) (CAT#: CBMAB-C9327-LY)

Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0403) (CAT#: CBMAB-C0444-FY)

Human CCR4 ELISA Kit (CAT#: V2LY-0626-LY580)

Human Anti-CCR4 Recombinant Antibody (CBXC-0785) (CAT#: CBMAB-C1899-CQ)

Human Anti-CCR4 Recombinant Antibody (
12F5) (CAT#: V2LY-0725-LY619)

Mouse Anti-CCR4 Recombinant Antibody (205410) (CAT#: CBMAB-C0585-LY)

Mouse Anti-CCR4 Recombinant Antibody (CBFYC-0428) (CAT#: CBMAB-C0474-FY)

Rabbit Anti-CCR4 Recombinant Antibody (CBFYC-1075) (CAT#: CBMAB-C1132-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry