Mouse Anti-CD200 Recombinant Antibody (CBT2730) (V2LY-0625-LY1739)

Name: Mouse Anti-CD200 Recombinant Antibody (CBT2730)
SKU: V2LY-0625-LY1739
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CD200 Recombinant Antibody (CBT2730) (V2LY-0625-LY1739) in ELISA

Mouse Anti-CD200 Recombinant Antibody (CBT2730) (V2LY-0625-LY1739) in WB

Mouse Anti-CD200 Recombinant Antibody (CBT2730) (V2LY-0625-LY1739) in FC

Mouse Anti-CD200 Recombinant Antibody (CBT2730) (V2LY-0625-LY1739) in IF

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2730

Application

ICC, FC

Immunogen

Purified recombinant fragment of human CD200 (AA:Extra(31-232)) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD200 Molecule

Entrez Gene ID

4345

UniProt ID

P41217

Alternative Names

CD200 Molecule; CD200 Antigen; Antigen Identified By Monoclonal Antibody MRC OX-2; MOX1; MOX2; OX-2 Membrane Glycoprotein; OX-2; MRC;

Function

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

Biological Process

Cell-cell adhesion Source: ARUK-UCL
Heterotypic cell-cell adhesion Source: ARUK-UCL
Negative regulation of cell population proliferation Source: ARUK-UCL
Negative regulation of interleukin-6 production Source: ARUK-UCL
Negative regulation of leukocyte activation Source: ARUK-UCL
Negative regulation of macrophage activation Source: ARUK-UCL
Negative regulation of macrophage migration Source: ARUK-UCL
Negative regulation of matrix metallopeptidase secretion Source: ARUK-UCL
Negative regulation of neuroinflammatory response Source: ARUK-UCL
Negative regulation of neuron death Source: ARUK-UCL
Negative regulation of NF-kappaB transcription factor activity Source: ARUK-UCL
Negative regulation of T cell migration Source: ARUK-UCL
Positive regulation of arginase activity Source: ARUK-UCL
Positive regulation of CREB transcription factor activity Source: ARUK-UCL
Positive regulation of protein-glutamine gamma-glutamyltransferase activity Source: ARUK-UCL
Positive regulation of transforming growth factor beta production Source: ARUK-UCL
Regulation of immune response Source: Reactome
Regulation of neuroinflammatory response Source: ARUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 31-232
Helical: 233-259
Cytoplasmic: 260-278

Liu, J. Q., Hu, A., Zhu, J., Yu, J., Talebian, F., & Bai, X. F. (2020). CD200-CD200R Pathway in the regulation of tumor immune microenvironment and immunotherapy. Tumor Microenvironment, 155-165.

Ismail, A. A., Donia, H. M., Ghatesh, H. M., & Farid, C. I. (2020). CD200/CD200 receptor axis in psoriasis vulgaris. PloS one, 15(3), e0230621.

Estep, R. D., Govindan, A. N., Fitzpatrick, K., Blair, T. C., Rezaee, S. R., Blackbourn, D. J., & Wong, S. W. (2020). Membrane-associated and secreted forms of the Rhesus macaque rhadinovirus-encoded CD200 homologue and cellular CD200 demonstrate differential effects on Rhesus Macaque CD200 Receptor signaling and regulation of myeloid cell activation. Journal of virology, 95(5), e01654-20.

Weng, L. C., Hou, S. H., Lei, S. T., Peng, H. Y., Li, M. Q., & Zhao, D. (2020). Estrogen-regulated CD200 inhibits macrophage phagocytosis in endometriosis. Journal of reproductive immunology, 138, 103090.

Manich, G., Recasens, M., Valente, T., Almolda, B., González, B., & Castellano, B. (2019). Role of the CD200-CD200R axis during homeostasis and neuroinflammation. Neuroscience, 405, 118-136.

Olin, M. R., Ampudia-Mesias, E., Pennell, C. A., Sarver, A., Chen, C. C., Moertel, C. L., ... & Pluhar, G. E. (2019). Treatment combining CD200 immune checkpoint inhibitor and tumor-lysate vaccination after surgery for pet dogs with high-grade glioma. Cancers, 11(2), 137.

Rexin, P., Tauchert, A., Haenze, J., Heers, H., Schmidt, A., Hofmann, R., & Hegele, A. (2018). The immune checkpoint molecule CD200 is associated with tumor grading and metastasis in bladder cancer. Anticancer research, 38(5), 2749-2754.

Chen, Z., Kapus, A., Khatri, I., Kos, O., Zhu, F., & Gorczynski, R. M. (2018). Cell membrane-bound CD200 signals both via an extracellular domain and following nuclear translocation of a cytoplasmic fragment. Leukemia research, 69, 72-80.

Nouri, F., Sadeghpour, H., Heidari, R., & Dehshahri, A. (2017). Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene. International journal of nanomedicine, 12, 5557.

Gorczynski, R. M., & Zhu, F. (2017). Checkpoint blockade in solid tumors and B-cell malignancies, with special consideration of the role of CD200. Cancer management and research, 9, 601.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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