Rat Anti-CD22 Recombinant Antibody (OX-97) (CBMAB-S0063-CQ)

Name: Rat Anti-CD22 Recombinant Antibody (OX-97)
SKU: CBMAB-S0063-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

OX-97

Application

Immunogen

Mouse CD22.

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CD22 Molecule

Introduction

CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Refractory Hairy Cell Leukemia. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Innate Immune System. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC1.

Entrez Gene ID

12483

UniProt ID

P35329

Alternative Names

CD22 Molecule; CD22 Antigen; Sialic Acid-Binding Ig-Like Lectin 2; B-Lymphocyte Cell Adhesion Molecule; T-Cell Surface Antigen Leu-14; SIGLEC-2;

Function

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Biological Process

B cell activation Source: ARUK-UCL
Cell adhesion Source: ProtInc
Negative regulation of B cell receptor signaling pathway Source: ARUK-UCL
Negative regulation of calcium-mediated signaling Source: ARUK-UCL
Negative regulation of immunoglobulin production Source: ARUK-UCL
Regulation of B cell proliferation Source: ARUK-UCL
Regulation of endocytosis Source: ARUK-UCL
Regulation of immune response Source: ARUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 20-687
Helical: 688-706
Cytoplasmic: 707-847

PTM

Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.

Spiegel, J. Y., Patel, S., Muffly, L., Hossain, N. M., Oak, J., Baird, J. H., ... & Miklos, D. B. (2021). CAR T cells with dual targeting of CD19 and CD22 in adult patients with recurrent or refractory B cell malignancies: a phase 1 trial. Nature Medicine, 27(8), 1419-1431.

Schneider, D., Xiong, Y., Wu, D., Hu, P., Alabanza, L., Steimle, B., ... & Dropulić, B. (2021). Trispecific CD19-CD20-CD22–targeting duoCAR-T cells eliminate antigen-heterogeneous B cell tumors in preclinical models. Science Translational Medicine, 13(586).

Lanza, F., Maffini, E., Rondoni, M., Massari, E., Faini, A. C., & Malavasi, F. (2020). CD22 expression in b-cell acute lymphoblastic leukemia: Biological significance and implications for inotuzumab therapy in adults. Cancers, 12(2), 303.

Nyesiga, B., & El-Schich, Z. (2020). CD22 (CD22 molecule). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

Wasim, L., Buhari, F. H. M., Yoganathan, M., Sicard, T., Ereño-Orbea, J., Julien, J. P., & Treanor, B. (2019). N-linked glycosylation regulates CD22 organization and function. Frontiers in immunology, 10, 699.

Clark, E. A., & Giltiay, N. V. (2018). CD22: a regulator of innate and adaptive B cell responses and autoimmunity. Frontiers in immunology, 9, 2235.

Sheikh, A. A. D., Akatsu, C., Imamura, A., Abdu-Allah, H. H., Takematsu, H., Ando, H., ... & Tsubata, T. (2018). Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2, 6 sialic acid-dependent and-independent interactions. Biochemical and biophysical research communications, 495(1), 854-859.

Pirosa, M. C., Leotta, S., Cupri, A., Stella, S., Martino, E. A., Scalise, L., ... & Milone, G. (2018). Long-term molecular remission achieved by antibody anti-cd22 and ponatinib in a patient affected by ph’+ acute lymphoblastic leukemia relapsed after second allogeneic hematopoietic stem cell transplantation: A case report. Chemotherapy, 63(4), 220-224.

Ereño-Orbea, J., Sicard, T., Cui, H., Mazhab-Jafari, M. T., Benlekbir, S., Guarné, A., ... & Julien, J. P. (2017). Molecular basis of human CD22 function and therapeutic targeting. Nature communications, 8(1), 1-11.

Peng, W., & Paulson, J. C. (2017). CD22 ligands on a natural N-glycan scaffold efficiently deliver toxins to B-lymphoma cells. Journal of the American Chemical Society, 139(36), 12450-12458.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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