Rhesus Recombinant SIGLEC2 protein, His Tag (V2LY-0526-LY9791)

Name: Rhesus Recombinant SIGLEC2 protein, His Tag
SKU: V2LY-0526-LY9791
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rhesus

Tag

His Tag

Protein Construction

This product is Rhesus Recombinant SIGLEC2 protein, His Tag consist of Amino Acid: 1-686 and predicts a molecular mass of 76.5 kDa.

Molecule Mass

76.5 kDa

Sequence

Amino Acid: 1-686

Species

Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol, DTT

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD22 Molecule

Alternative Names

CD22 Molecule; CD22 Antigen; Sialic Acid-Binding Ig-Like Lectin 2; B-Lymphocyte Cell Adhesion Molecule; T-Cell Surface Antigen Leu-14; SIGLEC-2;

Function

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Biological Process

B cell activation Source: ARUK-UCL
Cell adhesion Source: ProtInc
Negative regulation of B cell receptor signaling pathway Source: ARUK-UCL
Negative regulation of calcium-mediated signaling Source: ARUK-UCL
Negative regulation of immunoglobulin production Source: ARUK-UCL
Regulation of B cell proliferation Source: ARUK-UCL
Regulation of endocytosis Source: ARUK-UCL
Regulation of immune response Source: ARUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 20-687
Helical: 688-706
Cytoplasmic: 707-847

PTM

Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.

Spiegel, J. Y., Patel, S., Muffly, L., Hossain, N. M., Oak, J., Baird, J. H., ... & Miklos, D. B. (2021). CAR T cells with dual targeting of CD19 and CD22 in adult patients with recurrent or refractory B cell malignancies: a phase 1 trial. Nature Medicine, 27(8), 1419-1431.

Schneider, D., Xiong, Y., Wu, D., Hu, P., Alabanza, L., Steimle, B., ... & Dropulić, B. (2021). Trispecific CD19-CD20-CD22–targeting duoCAR-T cells eliminate antigen-heterogeneous B cell tumors in preclinical models. Science Translational Medicine, 13(586).

Lanza, F., Maffini, E., Rondoni, M., Massari, E., Faini, A. C., & Malavasi, F. (2020). CD22 expression in b-cell acute lymphoblastic leukemia: Biological significance and implications for inotuzumab therapy in adults. Cancers, 12(2), 303.

Nyesiga, B., & El-Schich, Z. (2020). CD22 (CD22 molecule). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

Wasim, L., Buhari, F. H. M., Yoganathan, M., Sicard, T., Ereño-Orbea, J., Julien, J. P., & Treanor, B. (2019). N-linked glycosylation regulates CD22 organization and function. Frontiers in immunology, 10, 699.

Clark, E. A., & Giltiay, N. V. (2018). CD22: a regulator of innate and adaptive B cell responses and autoimmunity. Frontiers in immunology, 9, 2235.

Sheikh, A. A. D., Akatsu, C., Imamura, A., Abdu-Allah, H. H., Takematsu, H., Ando, H., ... & Tsubata, T. (2018). Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2, 6 sialic acid-dependent and-independent interactions. Biochemical and biophysical research communications, 495(1), 854-859.

Pirosa, M. C., Leotta, S., Cupri, A., Stella, S., Martino, E. A., Scalise, L., ... & Milone, G. (2018). Long-term molecular remission achieved by antibody anti-cd22 and ponatinib in a patient affected by ph’+ acute lymphoblastic leukemia relapsed after second allogeneic hematopoietic stem cell transplantation: A case report. Chemotherapy, 63(4), 220-224.

Ereño-Orbea, J., Sicard, T., Cui, H., Mazhab-Jafari, M. T., Benlekbir, S., Guarné, A., ... & Julien, J. P. (2017). Molecular basis of human CD22 function and therapeutic targeting. Nature communications, 8(1), 1-11.

Peng, W., & Paulson, J. C. (2017). CD22 ligands on a natural N-glycan scaffold efficiently deliver toxins to B-lymphoma cells. Journal of the American Chemical Society, 139(36), 12450-12458.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD22 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY6778)

Human Recombinant CD22 protein, His Tag (CAT#: V2LY-0526-LY6779)

Human Recombinant CD22 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY6780)

Human Recombinant CD22 proteinECD (CAT#: V2LY-0526-LY6781)

Human Recombinant CD22 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY6782)

Human Recombinant CD22 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY6783)

Human Recombinant CD22 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY6784)

Human Recombinant CD22 protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY6785)

Human Recombinant CD22 protein, ECD, His Tag (CAT#: V2LY-0526-LY6786)

Human Recombinant CD22 protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY6787)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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