Mouse Anti-CD27 Recombinant Antibody (CBFYC-0005) (CBMAB-C0011-FY)

Name: Mouse Anti-CD27 Recombinant Antibody (CBFYC-0005)
SKU: CBMAB-C0011-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0005

Application

WB, IP, ELISA, IF, IHC-P

Immunogen

Amino acids1-260 of CD27 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CD27 Molecule

Introduction

CD27 (CD27 Molecule) is a Protein Coding gene. Diseases associated with CD27 include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Disease. Among its related pathways are Cytokine Signaling in Immune system and Akt Signaling. Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and cysteine-type endopeptidase inhibitor activity involved in apoptotic process.

Entrez Gene ID

939

UniProt ID

P26842

Alternative Names

CD27 Molecule

Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Biological Process

Cell surface receptor signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: BHF-UCL
Immunoglobulin mediated immune response Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of T cell apoptotic process Source: Ensembl
Positive regulation of B cell differentiation Source: UniProtKB
Positive regulation of JNK cascade Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of T cell differentiation Source: InterPro
Response to ethanol Source: Ensembl
Tumor necrosis factor-mediated signaling pathway Source: Reactome

Cellular Location

Membrane

Involvement in disease

Lymphoproliferative syndrome 2 (LPFS2): An autosomal recessive immunodeficiency disorder associated with persistent symptomatic EBV viremia, hypogammaglobulinemia, and impaired T-cell-dependent B-cell responses and T-cell dysfunction. The phenotype is highly variable, ranging from asymptomatic borderline-low hypogammaglobulinemia, to a full-blown symptomatic systemic inflammatory response with life-threatening EBV-related complications, including hemophagocytic lymphohistiocytosis, a lymphoproliferative disorder, and malignant lymphoma requiring stem cell transplantation.

Topology

Extracellular: 20-191
Helical: 192-212
Cytoplasmic: 213-260

PTM

Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.

Xu, F., Zhang, H., Si, X., Chen, J., Chen, Y., Cui, X., & Qin, Y. (2021). Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis. BMC immunology, 22(1), 1-8.

Deng, Y., Chatterjee, B., Zens, K., Zdimerova, H., Müller, A., Schuhmachers, P., ... & Münz, C. (2021). CD27 is required for protective lytic EBV antigen–specific CD8+ T-cell expansion. Blood, The Journal of the American Society of Hematology, 137(23), 3225-3236.

Rojas, J. M., Alejo, A., Avia, J. M., Rodríguez-Martín, D., Sánchez, C., Alcamí, A., ... & Martín, V. (2020). Activation of OX40 and CD27 costimulatory signalling in sheep through recombinant ovine ligands. Vaccines, 8(2), 333.

Nevalainen, T., Autio, A., Kummola, L., Salomaa, T., Junttila, I., Jylhä, M., & Hurme, M. (2019). CD27-IgD-B cell memory subset associates with inflammation and frailty in elderly individuals but only in males. Immunity & Ageing, 16(1), 1-9.

Starzer, A. M., & Berghoff, A. S. (2019). New emerging targets in cancer immunotherapy: CD27 (TNFRSF7). ESMO open, 4, e000629.

Centuori, S. M., Gomes, C. J., Kim, S. S., Putnam, C. W., Larsen, B. T., Garland, L. L., ... & Martinez, J. D. (2018). Double-negative (CD27− IgD−) B cells are expanded in NSCLC and inversely correlate with affinity-matured B cell populations. Journal of translational medicine, 16(1), 1-8.

Alaterre, E., Raimbault, S., Goldschmidt, H., Bouhya, S., Requirand, G., Robert, N., ... & Moreaux, J. (2017). CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma. Oncotarget, 8(58), 98931.

Burris, H. A., Infante, J. R., Ansell, S. M., Nemunaitis, J. J., Weiss, G. R., Villalobos, V. M., ... & Carson, W. E. (2017). Safety and activity of varlilumab, a novel and first-in-class agonist anti-CD27 antibody, in patients with advanced solid tumors. J Clin Oncol, 35(18), 2028-2036.

Chen, D., Gerasimčik, N., Camponeschi, A., Tan, Y., Wu, Q., Brynjolfsson, S., ... & Mårtensson, I. L. (2017). CD27 expression and its association with clinical outcome in children and adults with pro-B acute lymphoblastic leukemia. Blood cancer journal, 7(6), e575-e575.

Bullock, T. N. (2017). Stimulating CD27 to quantitatively and qualitatively shape adaptive immunity to cancer. Current opinion in immunology, 45, 82-88.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CD27 Matched Antibody Pair (213) (CAT#: APMAB-213LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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