Rat Anti-CD300A Recombinant Antibody (232612) (CBMAB-C0691-LY)

Name: Rat Anti-CD300A Recombinant Antibody (232612)
SKU: CBMAB-C0691-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

232612

Application

WB, FC

Immunogen

Y3 rat myeloid cell line transfected with human CD300a/LMIR1.

Host Species

Rat

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD300a Molecule

Introduction

CD300A (CD300a Molecule) is a Protein Coding gene. Diseases associated with CD300A include Kabuki Syndrome 1. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include phosphatidylserine binding and signaling receptor activity.
An important paralog of this gene is CD300C.

Entrez Gene ID

11314

UniProt ID

Q9UGN4

Function

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027).
Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923).

Biological Process

Cell adhesion Source: UniProtKB-KW
Negative regulation of activation of Janus kinase activity Source: UniProtKB
Negative regulation of B cell proliferation Source: UniProtKB
Negative regulation of B cell receptor signaling pathway Source: UniProtKB
Negative regulation of eosinophil activation Source: UniProtKB
Negative regulation of eosinophil migration Source: UniProtKB
Negative regulation of fibroblast proliferation Source: UniProtKB
Negative regulation of MAP kinase activity Source: UniProtKB
Negative regulation of mast cell activation involved in immune response Source: UniProtKB
Negative regulation of mast cell degranulation Source: UniProtKB
Negative regulation of MyD88-dependent toll-like receptor signaling pathway Source: UniProtKB
Negative regulation of neutrophil activation Source: UniProtKB
Negative regulation of NK T cell activation Source: UniProtKB
Negative regulation of phagocytosis, engulfment Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of phosphoprotein phosphatase activity Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of T cell receptor signaling pathway Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 18-180
Helical: 181-201
Cytoplasmic: 202-299

PTM

Phosphorylated on tyrosine.
N-glycosylated.

Nakahashi-Oda, C., Fujiyama, S., Nakazawa, Y., Kanemaru, K., Wang, Y., Lyu, W., ... & Shibuya, A. (2021). CD300a blockade enhances efferocytosis by infiltrating myeloid cells and ameliorates neuronal deficit after ischemic stroke. Science Immunology, 6(64), eabe7915.

Cao, Y., Ao, T., Wang, X., Wei, W., Fan, J., & Tian, X. (2021). CD300a and CD300f molecules regulate the function of leukocytes. International Immunopharmacology, 93, 107373.

Formentini, M., Navas, A., Hassouneh, F., Lopez-Sejas, N., Alonso, C., Tarazona, R., ... & Pera, A. (2021). Impact of CMV and age on T cell subsets defined by CD161, CD300a, and/or CD57 expression in healthy Andalusians. The Journals of Gerontology: Series A.

Barroso, R., Bicknell, R., Rainger, G. E., Watson, S. P., & Sun, Y. (2021). AVEXIS technology identifies novel platelet-leukocyte binding partners including CD148-CD300a. Blood Advances, 5(23), 5016-5019.

Vitallé, J., Terrén, I., Orrantia, A., Pérez-Garay, R., Vidal, F., Iribarren, J. A., ... & Borrego, F. (2019). CD300a inhibits CD16-mediated NK cell effector functions in HIV-1-infected patients. Cellular & molecular immunology, 16(12), 940-942.

Sun, X., Huang, S., Wang, X., Zhang, X., & Wang, X. (2018). CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia. Oncotarget, 9(44), 27574.

Vitallé, J., Terrén, I., Gamboa-Urquijo, L., Orrantia, A., Tarancón-Díez, L., Genebat, M., ... & Borrego, F. (2018). Altered expression of CD300a inhibitory receptor on CD4+ T cells from human immunodeficiency virus-1-infected patients: association with disease progression markers. Frontiers in immunology, 9, 1709.

Tang, Z., Cai, H., Wang, R., & Cui, Y. (2018). Overexpression of CD300A inhibits progression of NSCLC through downregulating Wnt/β-catenin pathway. OncoTargets and therapy, 11, 8875.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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