Human Recombinant CD300A protein, hFc Tag (V2LY-0526-LY2791)

Name: Human Recombinant CD300A protein, hFc Tag
SKU: V2LY-0526-LY2791
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CD300A protein, hFc Tag consist of Amino Acid: 1-178 and predicts a molecular mass of 44.5 kDa.

Molecule Mass

44.5 kDa

Sequence

Amino Acid: 1-178

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD300a Molecule

Function

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed:10746781, PubMed:16339535, PubMed:9701027).
Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed:22043923).

Biological Process

Cell adhesion Source: UniProtKB-KW
Negative regulation of activation of Janus kinase activity Source: UniProtKB
Negative regulation of B cell proliferation Source: UniProtKB
Negative regulation of B cell receptor signaling pathway Source: UniProtKB
Negative regulation of eosinophil activation Source: UniProtKB
Negative regulation of eosinophil migration Source: UniProtKB
Negative regulation of fibroblast proliferation Source: UniProtKB
Negative regulation of MAP kinase activity Source: UniProtKB
Negative regulation of mast cell activation involved in immune response Source: UniProtKB
Negative regulation of mast cell degranulation Source: UniProtKB
Negative regulation of MyD88-dependent toll-like receptor signaling pathway Source: UniProtKB
Negative regulation of neutrophil activation Source: UniProtKB
Negative regulation of NK T cell activation Source: UniProtKB
Negative regulation of phagocytosis, engulfment Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of phosphoprotein phosphatase activity Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of T cell receptor signaling pathway Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 18-180
Helical: 181-201
Cytoplasmic: 202-299

PTM

Phosphorylated on tyrosine.
N-glycosylated.

Nakahashi-Oda, C., Fujiyama, S., Nakazawa, Y., Kanemaru, K., Wang, Y., Lyu, W., ... & Shibuya, A. (2021). CD300a blockade enhances efferocytosis by infiltrating myeloid cells and ameliorates neuronal deficit after ischemic stroke. Science Immunology, 6(64), eabe7915.

Cao, Y., Ao, T., Wang, X., Wei, W., Fan, J., & Tian, X. (2021). CD300a and CD300f molecules regulate the function of leukocytes. International Immunopharmacology, 93, 107373.

Formentini, M., Navas, A., Hassouneh, F., Lopez-Sejas, N., Alonso, C., Tarazona, R., ... & Pera, A. (2021). Impact of CMV and age on T cell subsets defined by CD161, CD300a, and/or CD57 expression in healthy Andalusians. The Journals of Gerontology: Series A.

Barroso, R., Bicknell, R., Rainger, G. E., Watson, S. P., & Sun, Y. (2021). AVEXIS technology identifies novel platelet-leukocyte binding partners including CD148-CD300a. Blood Advances, 5(23), 5016-5019.

Vitallé, J., Terrén, I., Orrantia, A., Pérez-Garay, R., Vidal, F., Iribarren, J. A., ... & Borrego, F. (2019). CD300a inhibits CD16-mediated NK cell effector functions in HIV-1-infected patients. Cellular & molecular immunology, 16(12), 940-942.

Sun, X., Huang, S., Wang, X., Zhang, X., & Wang, X. (2018). CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia. Oncotarget, 9(44), 27574.

Vitallé, J., Terrén, I., Gamboa-Urquijo, L., Orrantia, A., Tarancón-Díez, L., Genebat, M., ... & Borrego, F. (2018). Altered expression of CD300a inhibitory receptor on CD4+ T cells from human immunodeficiency virus-1-infected patients: association with disease progression markers. Frontiers in immunology, 9, 1709.

Tang, Z., Cai, H., Wang, R., & Cui, Y. (2018). Overexpression of CD300A inhibits progression of NSCLC through downregulating Wnt/β-catenin pathway. OncoTargets and therapy, 11, 8875.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD300A protein, His Tag (CAT#: V2LY-0526-LY2792)

Mouse Recombinant CD300A protein, hFc Tag (CAT#: V2LY-0526-LY7991)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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