Mouse Anti-CD300LF Recombinant Antibody (CBT4249) (V2LY-0625-LY1708)

Name: Mouse Anti-CD300LF Recombinant Antibody (CBT4249)
SKU: V2LY-0625-LY1708
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CD300LF Recombinant Antibody (CBT4249) (V2LY-0625-LY1708) in ELISA

Mouse Anti-CD300LF Recombinant Antibody (CBT4249) (V2LY-0625-LY1708) in WB

Mouse Anti-CD300LF Recombinant Antibody (CBT4249) (V2LY-0625-LY1708) in FC

Mouse Anti-CD300LF Recombinant Antibody (CBT4249) (V2LY-0625-LY1708) in IHC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4249

Application

IHC, FC

Immunogen

Purified recombinant fragment of human CD300F (AA: Extra(20-156)) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD300 Molecule Like Family Member F

Entrez Gene ID

146722

UniProt ID

Q8TDQ1

Function

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731).
Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (By similarity).
Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150).
May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (By similarity).
Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923).
Inhibits osteoclast formation. Induces macrophage cell death upon engagement (By similarity).

Biological Process

Immune system process Source: UniProtKB-KW
Interleukin-13-mediated signaling pathway Source: UniProtKB
Negative regulation of apoptotic cell clearance Source: UniProtKB
Negative regulation of mast cell activation Source: UniProtKB
Negative regulation of MyD88-dependent toll-like receptor signaling pathway Source: UniProtKB
Positive regulation of apoptotic cell clearance Source: UniProtKB
Positive regulation of interleukin-4-mediated signaling pathway Source: UniProtKB
Regulation of immune response Source: Reactome
TRIF-dependent toll-like receptor signaling pathway Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 20-156
Helical: 157-177
Cytoplasmic: 178-290

PTM

Phosphorylated on tyrosine.

Graziano, V. R., Walker, F. C., Kennedy, E. A., Wei, J., Ettayebi, K., Strine, M. S., ... & Wilen, C. B. (2020). CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus. PLoS pathogens, 16(4), e1008242.

Furlong, K., Biering, S. B., Choi, J., Wilen, C. B., Orchard, R. C., Wobus, C. E., ... & Hwang, S. (2020). CD300LF polymorphisms of inbred mouse strains confer resistance to murine norovirus infection in a cell type-dependent manner. Journal of virology, 94(17), e00837-20.

Keswani, T., Roland, J., Herbert, F., Delcroix-Genete, D., Bauderlique-Le Roy, H., Gaayeb, L., ... & Pied, S. (2020). Expression of CD300lf by microglia contributes to resistance to cerebral malaria by impeding the neuroinflammation. Genes & Immunity, 21(1), 45-62.

Kilic, T., Koromyslova, A., Malak, V., & Hansman, G. S. (2018). Atomic structure of the murine norovirus protruding domain and soluble CD300lf receptor complex. Journal of virology, 92(11), e00413-18.

Nelson, C. A., Wilen, C. B., Dai, Y. N., Orchard, R. C., Kim, A. S., Stegeman, R. A., ... & Fremont, D. H. (2018). Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor. Proceedings of the National Academy of Sciences, 115(39), E9201-E9210.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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