Human Recombinant CD300F protein, His Tag (V2LY-0526-LY2796)

Name: Human Recombinant CD300F protein, His Tag
SKU: V2LY-0526-LY2796
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD300F protein, His Tag consist of Amino Acid: 1-155 and predicts a molecular mass of 16.9 kDa.

Molecule Mass

16.9 kDa

Verified

HPLC

Sequence

Amino Acid: 1-155

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD300 Molecule Like Family Member F

Function

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731).
Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (By similarity).
Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150).
May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (By similarity).
Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923).
Inhibits osteoclast formation. Induces macrophage cell death upon engagement (By similarity).

Biological Process

Immune system process Source: UniProtKB-KW
Interleukin-13-mediated signaling pathway Source: UniProtKB
Negative regulation of apoptotic cell clearance Source: UniProtKB
Negative regulation of mast cell activation Source: UniProtKB
Negative regulation of MyD88-dependent toll-like receptor signaling pathway Source: UniProtKB
Positive regulation of apoptotic cell clearance Source: UniProtKB
Positive regulation of interleukin-4-mediated signaling pathway Source: UniProtKB
Regulation of immune response Source: Reactome
TRIF-dependent toll-like receptor signaling pathway Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 20-156
Helical: 157-177
Cytoplasmic: 178-290

PTM

Phosphorylated on tyrosine.

Graziano, V. R., Walker, F. C., Kennedy, E. A., Wei, J., Ettayebi, K., Strine, M. S., ... & Wilen, C. B. (2020). CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus. PLoS pathogens, 16(4), e1008242.

Furlong, K., Biering, S. B., Choi, J., Wilen, C. B., Orchard, R. C., Wobus, C. E., ... & Hwang, S. (2020). CD300LF polymorphisms of inbred mouse strains confer resistance to murine norovirus infection in a cell type-dependent manner. Journal of virology, 94(17), e00837-20.

Keswani, T., Roland, J., Herbert, F., Delcroix-Genete, D., Bauderlique-Le Roy, H., Gaayeb, L., ... & Pied, S. (2020). Expression of CD300lf by microglia contributes to resistance to cerebral malaria by impeding the neuroinflammation. Genes & Immunity, 21(1), 45-62.

Kilic, T., Koromyslova, A., Malak, V., & Hansman, G. S. (2018). Atomic structure of the murine norovirus protruding domain and soluble CD300lf receptor complex. Journal of virology, 92(11), e00413-18.

Nelson, C. A., Wilen, C. B., Dai, Y. N., Orchard, R. C., Kim, A. S., Stegeman, R. A., ... & Fremont, D. H. (2018). Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor. Proceedings of the National Academy of Sciences, 115(39), E9201-E9210.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD300F protein, hFc Tag (CAT#: V2LY-0526-LY2795)

Mouse Recombinant CD300LF protein, ECD, Fc Tag (CAT#: V2LY-0526-LY7993)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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