Rabbit Anti-CD8A Recombinant Antibody (
2F2) (V2LY-0725-LY1493)

Name: Rabbit Anti-CD8A Recombinant Antibody (2F2)
SKU: V2LY-0725-LY1493
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CD8A Recombinant Antibody (2F2) (V2LY-0725-LY1493) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: CD8A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 22, 31 kDa
Observed band size: 31 kDa

Rabbit Anti-CD8A Recombinant Antibody (2F2) (V2LY-0725-LY1493) in IHC

IHC image of V2LY-0725-LY1493 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-CD8A Recombinant Antibody (2F2) (V2LY-0725-LY1493) in IHC

IHC image of V2LY-0725-LY1493 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-CD8A Recombinant Antibody (2F2) (V2LY-0725-LY1493) in IP

Immunoprecipitating CD8A in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY1493 in HepG2 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2:V2LY-0725-LY1493(3µg)+ HepG2 whole cell lysate(500µg)
Lane 3: HepG2 whole cell lysate (10µg)

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

2F2

Application

ELISA, WB, IHC, IP

Immunogen

A synthesized peptide derived from human CD8.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD8a Molecule

Entrez Gene ID

925

UniProt ID

P01732

Alternative Names

CD8a Molecule; T-Lymphocyte Differentiation Antigen T8/Leu-2; T-Cell Surface Glycoprotein CD8 Alpha Chain; CD8 Antigen, Alpha Polypeptide (P32); MAL; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2;

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Biological Process

Antigen processing and presentation Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Cytotoxic T cell differentiation Source: GO_Central
Immune response Source: UniProtKB
Regulation of immune response Source: Reactome
T cell activation Source: UniProtKB
T cell mediated immunity Source: GO_Central
Transmembrane receptor protein tyrosine kinase signaling pathway Source: UniProtKB

Cellular Location

Isoform 1: Cell membrane. CD8A localizes to lipid rafts only when associated with its partner CD8B.
Isoform 2: Secreted

Involvement in disease

CD8 deficiency, familial (CD8 deficiency): An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Topology

Extracellular: 22-182
Helical: 183-203
Cytoplasmic: 204-235

PTM

Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts.
O-glycosylated.
Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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