Mouse Anti-CDA Recombinant Antibody (C4871) (CBMAB-C4871-CQ)

Name: Mouse Anti-CDA Recombinant Antibody (C4871)
SKU: CBMAB-C4871-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDA Recombinant Antibody (C4871) (CBMAB-C4871-CQ) in WB

CDA protein expression analyzed by Western blots in neuroblastoma cell lines and lung cancer cell lines. ...View More

Alkasalias, T., Zhang, J., Madapura, H., Dalarun, B., Reina, O. B., Lewensohn, R., ... & Laín, S. (2022). Proof-of-principle studies on a strategy to enhance nucleotide imbalance specifically in cancer cells. Cell Death Discovery, 8(1), 464.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDA Recombinant Antibody (C4871) (CBMAB-C4871-CQ) in WB

CNT3-Ins represents a CNT3 splice isoform with ER localization. WB analysis was used to evaluate CDA protein expression in K562 and KAR-0.2 cells. Compared to HeLa cells, using CRT as loading control. ...View More

Levin, M., Stark, M., Berman, B., & Assaraf, Y. G. (2019). Surmounting Cytarabine-resistance in acute myeloblastic leukemia cells and specimens with a synergistic combination of hydroxyurea and azidothymidine. Cell Death & Disease, 10(6), 390.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDA Recombinant Antibody (C4871) (CBMAB-C4871-CQ) in WB

Protein expression of dCK and CDA was quantified by western blotting in MCF7-wt and MCF7-R cells, β-actin was used as an internal control. An altered expression of both dCK and CDA was observed in MCF7-R cells in comparison to the wild-type counterpart. The gene expression of dCK was decreased to 2.7-fold in MCF7-R compared with wild-type MCF7, whereas the expression of CDA was 187-fold higher in MCF7-R cells compared with near basal level expression of this enzyme in MCF7 wild-type. ...View More

Fatima, M., Ahmed, M. M. I., Batool, F., Riaz, A., Ali, M., Munch-Petersen, B., & Mutahir, Z. (2019). Recombinant deoxyribonucleoside kinase from Drosophila melanogaster can improve gemcitabine based combined gene/chemotherapy for targeting cancer cells. Bosnian journal of basic medical sciences, 19(4), 342.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-CDA Recombinant Antibody (C4871) (CBMAB-C4871-CQ) in Immunoblot

Immunoblots showing APOBEC3A (A3A), APOBEC3G (A3G) and CDA proteins in whole-cell lysates (20 μg protein) of 293T cells transiently transfected with an empty vector (Ctrl., control) or DNA constructs for expression of A3A, A3G or CDA proteins. ...View More

Sharma, S., Patnaik, S. K., Thomas Taggart, R., Kannisto, E. D., Enriquez, S. M., Gollnick, P., & Baysal, B. E. (2015). APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages. Nature communications, 6(1), 6881.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C4871

Application

WB, IF, IP, ELISA, IHC-P

Immunogen

Amino acids 1-146 representing full length CDD of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cytidine Deaminase

Introduction

CDA (Cytidine Deaminase) is a Protein Coding gene. Diseases associated with CDA include Refractory Hematologic Cancer and Refractory Hairy Cell Leukemia. Among its related pathways are Pyrimidine metabolism (KEGG) and Innate Immune System. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and hydrolase activity.

Entrez Gene ID

978

UniProt ID

P32320

Alternative Names

Cytidine Deaminase; Cytidine Aminohydrolase; CDD; Cytosine Nucleoside Deaminase; Small Cytidine Deaminase; EC 3.5.4.5;

Function

This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.

Biological Process

Cell surface receptor signaling pathway Source: UniProtKB
Cytidine deamination Source: UniProtKB
Cytosine metabolic process Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of nucleotide metabolic process Source: UniProtKB
Neutrophil degranulation Source: Reactome
Pyrimidine-containing compound salvage Source: UniProtKB
Pyrimidine nucleoside salvage Source: Reactome

Cellular Location

Cytosol; Extracellular region; Ficolin-1-rich granule lumen; Secretory granule lumen; Tertiary granule lumen

Frances, A., & Cordelier, P. (2020). The emerging role of cytidine deaminase in human diseases: a new opportunity for therapy?. Molecular Therapy, 28(2), 357-366.

Bjånes, T. K., Jordheim, L. P., Schjøtt, J., Kamceva, T., Cros-Perrial, E., Langer, A., ... & Riedel, B. (2020). Intracellular cytidine deaminase regulates gemcitabine metabolism in pancreatic cancer cell lines. Drug Metabolism and Disposition, 48(3), 153-158.

Murakami, Y., Kimura, Y., Kawahara, A., Mitsuyasu, S., Miyake, H., Tohyama, K., ... & Kuwano, M. (2019). The augmented expression of the cytidine deaminase gene by 5-azacytidine predicts therapeutic efficacy in myelodysplastic syndromes. Oncotarget, 10(23), 2270.

Liu, W., Shang, F., Chen, Y., Lan, J., Wang, L., Chen, J., ... & Xu, Y. (2019). Biochemical and structural analysis of the Klebsiella pneumoniae cytidine deaminase CDA. Biochemical and biophysical research communications, 519(2), 280-286.

Moro-Bulnes, A., Castillo-Acosta, V. M., Valente, M., Carrero-Lérida, J., Pérez-Moreno, G., Ruiz-Pérez, L. M., & González-Pacanowska, D. (2019). Contribution of cytidine deaminase to thymidylate biosynthesis in trypanosoma brucei: intracellular localization and properties of the enzyme. Msphere, 4(4), e00374-19.

Peters, G. J., Giovannetti, E., Honeywell, R. J., & Ciccolini, J. (2019). Can cytidine deaminase be used as predictive biomarker for gemcitabine toxicity and response?. British journal of clinical pharmacology, 85(6), 1213.

Cohen, R., Preta, L. H., Joste, V., Curis, E., Huillard, O., Jouinot, A., ... & Blanchet, B. (2019). Determinants of the interindividual variability in serum cytidine deaminase activity of patients with solid tumours. British journal of clinical pharmacology, 85(6), 1227-1238.

Fanciullino, R., Farnault, L., Donnette, M., Imbs, D. C., Roche, C., Venton, G., ... & Costello, R. (2018). CDA as a predictive marker for life-threatening toxicities in patients with AML treated with cytarabine. Blood Advances, 2(5), 462-469.

Rajabpour, A., Afgar, A., Mahmoodzadeh, H., Radfar, J. E. D., Rajaei, F., & Teimoori-Toolabi, L. (2017). MiR-608 regulating the expression of ribonucleotide reductase M1 and cytidine deaminase is repressed through induced gemcitabine chemoresistance in pancreatic cancer cells. Cancer chemotherapy and pharmacology, 80(4), 765-775.

Corrie, P., Qian, W., Gopinathan, A., Williams, M., Brais, R., Valle, J. W., ... & Jodrell, D. (2017). Strong tumour cytidine deaminase (CDA) staining predicts for improved survival associated with sequential nab-Paclitaxel (nabP) and gemcitabine (GEM) chemotherapy as first line treatment of patients (pts) with metastatic pancreatic adenocarcinoma (mPDAC). Annals of Oncology, 28, v251-v252.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CDA Matched Antibody Pair (242) (CAT#: APMAB-242LY)

Related Products

CDA Matched Antibody Pair (242) (CAT#: APMAB-242LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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