Mouse Anti-CDC27 Recombinant Antibody (C0617) (CBMAB-C0617-LY)

Name: Mouse Anti-CDC27 Recombinant Antibody (C0617)
SKU: CBMAB-C0617-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDC27 Recombinant Antibody (C0617) (CBMAB-C0617-LY) in Immunoblot and IP

Lysates of asynchronous HeLa cells were immunoprecipitated (IP) with an anti-SIRT6 antibody or normal IgG, and then the immunoprecipitates were analyzed by immunoblotting. ...View More

Wang, H., Feng, K., Wang, Q., & Deng, H. (2021). Reciprocal interaction between SIRT6 and APC/C regulates genomic stability. Scientific Reports, 11(1), 14253.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Frog, Horse, Dog

Clone

C0617

Antibody Isotype

IgG2b, κ

Application

WB, IF, IP, IHC-P

Basic Information

Immunogen

Amino acids 814-823 of Cdc27 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Frog, Horse, Dog

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cell Division Cycle 27

Introduction

CDC27 (Cell Division Cycle 27) is a Protein Coding gene. Diseases associated with CDC27 include Cervix Carcinoma and Breast Cancer. Among its related pathways are PAK Pathway and Actin Nucleation by ARP-WASP Complex. Gene Ontology (GO) annotations related to this gene include protein phosphatase binding.

Entrez Gene ID

996

UniProt ID

P30260

Alternative Names

Cell Division Cycle 27; Anaphase Promoting Complex Subunit 3; D0S1430E, D17S978E; D0S1430E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3;

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: GO_Central
Cell division Source: GO_Central
Metaphase/anaphase transition of mitotic cell cycle Source: MGI
Positive regulation of mitotic metaphase/anaphase transition Source: GO_Central
Protein K11-linked ubiquitination Source: UniProtKB
Protein ubiquitination Source: GO_Central
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Nucleus; Spindle

PTM

Phosphorylated. Phosphorylation on Ser-426 and Thr-446 ^oCcurs specifically during mitosis.

More Infomation

References

Kazemi-Sefat, G. E., Keramatipour, M., Talebi, S., Kavousi, K., Sajed, R., Kazemi-Sefat, N. A., & Mousavizadeh, K. (2021). The importance of CDC27 in cancer: molecular pathology and clinical aspects. Cancer cell international, 21(1), 1-11.

Hu, Y., Fu, N., Chen, L. X., Xiao, J. H., & Yang, X. F. (2021). COX-2 Regulates the Proliferation and Apoptosis of Activated Hepatic Stellate Cells through CDC27. Journal of Nanomaterials, 2021.

Abbaspourkharyeki, M., Anvekar, N. J., & Ramachandra, N. B. (2020). The Possible Role of Point Mutations and Activation of the CDC27 Gene in Progression of Multiple Myeloma. Meta Gene, 26, 100761.

Song, Y., Song, W., Li, Z., Song, W., Wen, Y., Li, J., ... & Zhang, M. (2020). CDC27 promotes tumor progression and affects PD-L1 expression in T-Cell Lymphoblastic lymphoma. Frontiers in oncology, 10, 488.

Wu, R., Li, Q., Wu, F., Shi, C., & Chen, Q. (2020). Comprehensive analysis of CDC27 related to peritoneal metastasis by whole exome sequencing in gastric cancer. OncoTargets and therapy, 13, 3335.

Qi, F., Li, Y., Yang, X., Wu, Y., Lin, L., & Liu, X. (2020). Hsa_circ_0044226 knockdown attenuates progression of pulmonary fibrosis by inhibiting CDC27. Aging (Albany NY), 12(14), 14808.

Xin, Y., Ning, S., Zhang, L., & Cui, M. (2018). CDC27 facilitates gastric cancer cell proliferation, invasion and metastasis via twist-induced epithelial-mesenchymal transition. Cellular Physiology and Biochemistry, 50(2), 501-511.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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