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Mouse Anti-CDK8 Recombinant Antibody (CBYY-C1787) (CBMAB-C3225-YY)

This product is mouse antibody that recognizes CDK8. The antibody CBYY-C1787 can be used for immunoassay techniques such as: ELISA, WB
See all CDK8 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C1787
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein corresponding to aa375-464 from human CDK8 with GST tag. MW of the GST tag alone is 26kD
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Preservative
PBS, pH 7.4
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin Dependent Kinase 8
Entrez Gene ID
Human1024
Mouse264064
Rat498140
UniProt ID
HumanP49336
MouseQ8R3L8
Alternative Names
Cyclin Dependent Kinase Inhibitor 2C; Cyclin-Dependent Kinase Inhibitor 2C (P18, Inhibits CDK4); P18-INK4C; P18-INK6; Cyclin-Dependent Kinase 6 Inhibitor P18; Cyclin-Dependent Kinase 4 Inhibitor C; Cyclin-Dependent Kinase 6 Inhibitor;
Function
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Biological Process
Positive regulation of transcription by RNA polymerase II Source: Reactome
Protein phosphorylation Source: GO_Central
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
Cellular Location
Nucleus
Involvement in disease
Intellectual developmental disorder with hypotonia and behavioral abnormalities (IDDHBA): An autosomal dominant neurodevelopmental disorder with onset in infancy. IDDHBA is characterized by hypotonia, global developmental delay, learning disability, and behavioral abnormalities, such as autistic features and attention deficit-hyperactivity disorder. Additional variable features may include non-specific facial dysmorphism, congenital heart defects, ocular anomalies, and poor feeding.

Chong, L., Shi, X., & Zhu, Y. (2021). Signal integration by cyclin-dependent kinase 8 (CDK8) module and other mediator subunits in biotic and abiotic stress responses. International Journal of Molecular Sciences, 22(1), 354.

Piya, S., Ma, H., Ruvolo, V., Basyal, M., Golas, A., Windak, R., ... & Borthakur, G. (2021). Inhibition of Cyclin Dependent Kinase 8 (CDK8): A Novel Approach to Target the Leukemia Initiating Cells (LICs) in T-Cell Acute Lymphoblastic Leukaemia (T-ALL). Blood, 138(Supplement 1), 2250-2250.

Elkady, M. A., Doghish, A. S., Elshafei, A., & Elshafey, M. M. (2021). MicroRNA-567 inhibits cell proliferation and induces cell apoptosis in A549 NSCLC cells by regulating cyclin-dependent kinase 8. Saudi Journal of Biological Sciences, 28(4), 2581-2590.

Xi, X., Yao, Y., Liu, N., & Li, P. (2020). MiR-297 alleviates LPS-induced A549 cell and mice lung injury via targeting cyclin dependent kinase 8. International immunopharmacology, 80, 106197.

Liu, T., Meng, J., & Zhang, Y. (2020). miR-592 acts as an oncogene and promotes medullary thyroid cancer tumorigenesis by targeting cyclin-dependent kinase 8. Molecular Medicine Reports, 22(4), 3316.

Lv, X., Tian, Y., Li, S., Cheng, K., Huang, X., Kong, H., ... & Xie, Z. (2020). Discovery and Development of Cyclin-Dependent Kinase 8 Inhibitors. Current medicinal chemistry, 27(32), 5429-5443.

Yin, T., Liu, M. M., Jin, R. T., Kong, J., Wang, S. H., & Sun, W. B. (2019). miR-152-3p Modulates hepatic carcinogenesis by targeting cyclin-dependent kinase 8. Pathology-Research and Practice, 215(6), 152406.

Amirhosseini, M., Bernhardsson, M., Lång, P., Andersson, G., Flygare, J., & Fahlgren, A. (2019). Cyclin‐dependent kinase 8/19 inhibition suppresses osteoclastogenesis by downregulating RANK and promotes osteoblast mineralization and cancellous bone healing. Journal of cellular physiology, 234(9), 16503-16516.

Han, C., Zheng, W., Ge, M., Wang, K., Xiang, Y., & Wang, P. (2017). Downregulation of cyclin-dependent kinase 8 by microRNA-148a suppresses proliferation and invasiveness of papillary thyroid carcinomas. American journal of cancer research, 7(10), 2081.

Philip, S., Kumarasiri, M., Teo, T., Yu, M., & Wang, S. (2017). Cyclin-dependent kinase 8: a new hope in targeted cancer therapy? Miniperspective. Journal of medicinal chemistry, 61(12), 5073-5092.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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