Rabbit Anti-CDK8 Recombinant Antibody (EG614) (CBMAB-EN739-LY)

Name: Rabbit Anti-CDK8 Recombinant Antibody (EG614)
SKU: CBMAB-EN739-LY
Rating: 5 (1 reviews)

See all CDK8 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG614

Application

Immunogen

Synthetic peptide within Human CDK8 aa 1-50.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

TBS, pH 7.4, 40% Glycerol, 0.05% BSA

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cyclin Dependent Kinase 8

Introduction

CDK8 (Cyclin Dependent Kinase 8) is a Protein Coding gene. Diseases associated with CDK8 include Colorectal Adenocarcinoma and Hemophagocytic Lymphohistiocytosis, Familial, 3. Among its related pathways are Signaling by NOTCH1 and Notch Signaling Pathway (sino). Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDK19.

Entrez Gene ID

Human	1024
Mouse	264064

UniProt ID

Human	P49336
Mouse	Q8R3L8

Alternative Names

Cyclin Dependent Kinase 8; Mediator Of RNA Polymerase II Transcription Subunit CDK8; Cell Division Protein Kinase 8; Mediator Complex Subunit CDK8; Cyclin-Dependent Kinase 8; Protein Kinase K35;

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

Biological Process

Positive regulation of transcription by RNA polymerase II Source: Reactome
Protein phosphorylation Source: GO_Central
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome

Cellular Location

Nucleus

Involvement in disease

Intellectual developmental disorder with hypotonia and behavioral abnormalities (IDDHBA): An autosomal dominant neurodevelopmental disorder with onset in infancy. IDDHBA is characterized by hypotonia, global developmental delay, learning disability, and behavioral abnormalities, such as autistic features and attention deficit-hyperactivity disorder. Additional variable features may include non-specific facial dysmorphism, congenital heart defects, ocular anomalies, and poor feeding.

Chong, L., Shi, X., & Zhu, Y. (2021). Signal integration by cyclin-dependent kinase 8 (CDK8) module and other mediator subunits in biotic and abiotic stress responses. International Journal of Molecular Sciences, 22(1), 354.

Piya, S., Ma, H., Ruvolo, V., Basyal, M., Golas, A., Windak, R., ... & Borthakur, G. (2021). Inhibition of Cyclin Dependent Kinase 8 (CDK8): A Novel Approach to Target the Leukemia Initiating Cells (LICs) in T-Cell Acute Lymphoblastic Leukaemia (T-ALL). Blood, 138(Supplement 1), 2250-2250.

Elkady, M. A., Doghish, A. S., Elshafei, A., & Elshafey, M. M. (2021). MicroRNA-567 inhibits cell proliferation and induces cell apoptosis in A549 NSCLC cells by regulating cyclin-dependent kinase 8. Saudi Journal of Biological Sciences, 28(4), 2581-2590.

Xi, X., Yao, Y., Liu, N., & Li, P. (2020). MiR-297 alleviates LPS-induced A549 cell and mice lung injury via targeting cyclin dependent kinase 8. International immunopharmacology, 80, 106197.

Liu, T., Meng, J., & Zhang, Y. (2020). miR-592 acts as an oncogene and promotes medullary thyroid cancer tumorigenesis by targeting cyclin-dependent kinase 8. Molecular Medicine Reports, 22(4), 3316.

Lv, X., Tian, Y., Li, S., Cheng, K., Huang, X., Kong, H., ... & Xie, Z. (2020). Discovery and Development of Cyclin-Dependent Kinase 8 Inhibitors. Current medicinal chemistry, 27(32), 5429-5443.

Yin, T., Liu, M. M., Jin, R. T., Kong, J., Wang, S. H., & Sun, W. B. (2019). miR-152-3p Modulates hepatic carcinogenesis by targeting cyclin-dependent kinase 8. Pathology-Research and Practice, 215(6), 152406.

Amirhosseini, M., Bernhardsson, M., Lång, P., Andersson, G., Flygare, J., & Fahlgren, A. (2019). Cyclin‐dependent kinase 8/19 inhibition suppresses osteoclastogenesis by downregulating RANK and promotes osteoblast mineralization and cancellous bone healing. Journal of cellular physiology, 234(9), 16503-16516.

Han, C., Zheng, W., Ge, M., Wang, K., Xiang, Y., & Wang, P. (2017). Downregulation of cyclin-dependent kinase 8 by microRNA-148a suppresses proliferation and invasiveness of papillary thyroid carcinomas. American journal of cancer research, 7(10), 2081.

Philip, S., Kumarasiri, M., Teo, T., Yu, M., & Wang, S. (2017). Cyclin-dependent kinase 8: a new hope in targeted cancer therapy? Miniperspective. Journal of medicinal chemistry, 61(12), 5073-5092.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-CDK8 Recombinant Antibody (EG614)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CDK8 Recombinant Antibody (6E5) (CAT#: CBMAB-C2133-CN)

Mouse Anti-CDK8 Recombinant Antibody (CBYY-C1787) (CAT#: CBMAB-C3225-YY)

Mouse Anti-CDK8 Recombinant Antibody (CP0270) (CAT#: CBMAB-CP0270-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry