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Rabbit Anti-CDK8 Recombinant Antibody (D6M3J) (CBMAB-CP0270-LY)

The product is antibody recognizes CDK8. The antibody D6M3J immunoassay techniques such as: WB,IP.
See all CDK8 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Monkey
Clone
D6M3J
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human CDK8 protein.
Specificity
Human, Mouse, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cyclin Dependent Kinase 8
Introduction
CDK8 (Cyclin Dependent Kinase 8) is a Protein Coding gene. Diseases associated with CDK8 include Colorectal Adenocarcinoma and Hemophagocytic Lymphohistiocytosis, Familial, 3. Among its related pathways are Signaling by NOTCH1 and Notch Signaling Pathway (sino). Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDK19.
Entrez Gene ID
Human1024
Mouse264064
Monkey709761
UniProt ID
HumanP49336
MouseQ8R3L8
MonkeyA0A0D9RZG8
Alternative Names
Cyclin Dependent Kinase 8; Mediator Of RNA Polymerase II Transcription Subunit CDK8; Cell Division Protein Kinase 8; Mediator Complex Subunit CDK8; Cyclin-Dependent Kinase 8; Protein Kinase K35;
Function
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Biological Process
Positive regulation of transcription by RNA polymerase II Source: Reactome
Protein phosphorylation Source: GO_Central
Regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
Cellular Location
Nucleus
Involvement in disease
Intellectual developmental disorder with hypotonia and behavioral abnormalities (IDDHBA): An autosomal dominant neurodevelopmental disorder with onset in infancy. IDDHBA is characterized by hypotonia, global developmental delay, learning disability, and behavioral abnormalities, such as autistic features and attention deficit-hyperactivity disorder. Additional variable features may include non-specific facial dysmorphism, congenital heart defects, ocular anomalies, and poor feeding.

Chong, L., Shi, X., & Zhu, Y. (2021). Signal integration by cyclin-dependent kinase 8 (CDK8) module and other mediator subunits in biotic and abiotic stress responses. International Journal of Molecular Sciences, 22(1), 354.

Piya, S., Ma, H., Ruvolo, V., Basyal, M., Golas, A., Windak, R., ... & Borthakur, G. (2021). Inhibition of Cyclin Dependent Kinase 8 (CDK8): A Novel Approach to Target the Leukemia Initiating Cells (LICs) in T-Cell Acute Lymphoblastic Leukaemia (T-ALL). Blood, 138(Supplement 1), 2250-2250.

Elkady, M. A., Doghish, A. S., Elshafei, A., & Elshafey, M. M. (2021). MicroRNA-567 inhibits cell proliferation and induces cell apoptosis in A549 NSCLC cells by regulating cyclin-dependent kinase 8. Saudi Journal of Biological Sciences, 28(4), 2581-2590.

Xi, X., Yao, Y., Liu, N., & Li, P. (2020). MiR-297 alleviates LPS-induced A549 cell and mice lung injury via targeting cyclin dependent kinase 8. International immunopharmacology, 80, 106197.

Liu, T., Meng, J., & Zhang, Y. (2020). miR-592 acts as an oncogene and promotes medullary thyroid cancer tumorigenesis by targeting cyclin-dependent kinase 8. Molecular Medicine Reports, 22(4), 3316.

Lv, X., Tian, Y., Li, S., Cheng, K., Huang, X., Kong, H., ... & Xie, Z. (2020). Discovery and Development of Cyclin-Dependent Kinase 8 Inhibitors. Current medicinal chemistry, 27(32), 5429-5443.

Yin, T., Liu, M. M., Jin, R. T., Kong, J., Wang, S. H., & Sun, W. B. (2019). miR-152-3p Modulates hepatic carcinogenesis by targeting cyclin-dependent kinase 8. Pathology-Research and Practice, 215(6), 152406.

Amirhosseini, M., Bernhardsson, M., Lång, P., Andersson, G., Flygare, J., & Fahlgren, A. (2019). Cyclin‐dependent kinase 8/19 inhibition suppresses osteoclastogenesis by downregulating RANK and promotes osteoblast mineralization and cancellous bone healing. Journal of cellular physiology, 234(9), 16503-16516.

Han, C., Zheng, W., Ge, M., Wang, K., Xiang, Y., & Wang, P. (2017). Downregulation of cyclin-dependent kinase 8 by microRNA-148a suppresses proliferation and invasiveness of papillary thyroid carcinomas. American journal of cancer research, 7(10), 2081.

Philip, S., Kumarasiri, M., Teo, T., Yu, M., & Wang, S. (2017). Cyclin-dependent kinase 8: a new hope in targeted cancer therapy? Miniperspective. Journal of medicinal chemistry, 61(12), 5073-5092.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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