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Mouse Anti-CDKN1C Monoclonal Antibody (R0027) (CBMAB-R0027-FY)

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Published Data

CBMAB-R0027-FY-1

Mouse Anti-CDKN1C Monoclonal Antibody (R0027) (CBMAB-R0027-FY) in IF

NFE2L1, NQO1 and synaptopodin expression in untreated and PAN-treated ex vivo human kidney slices. A representative image of a glomerulus from ex vivo kidney slices, untreated slices from normal kidney parenchyma and treated with concentrations of 10, 20 and 30 μg/mL for 24 h. Sections were multiplexed with Hoechst (blue), p57 (green), synaptopodin (red) NFE2L1 (yellow) and NQO1 (purple) antibodies. Magnification = 20× with scale bar representing 20 μm. ...View More

Elshani, M., Um, I. H., Leung, S., Reynolds, P. A., Chapman, A., Kudsy, M., & Harrison, D. J. (2023). Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage. Cells, 12(17), 2165.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-R0027-FY-2

Mouse Anti-CDKN1C Monoclonal Antibody (R0027) (CBMAB-R0027-FY) in WB

Representative western blot of CDKN1C and DLK1 in primary pancreatic islet cells treated with or without 30 mM glucose (HG) for 48 h. ...View More

Yang, T., Yan, C., Yang, L., Tan, J., Jiang, S., Hu, J., ... & Li, Y. (2023). Identification and validation of core genes for type 2 diabetes mellitus by integrated analysis of single-cell and bulk RNA-sequencing. European Journal of Medical Research, 28(1), 340.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-R0027-FY-3

Mouse Anti-CDKN1C Monoclonal Antibody (R0027) (CBMAB-R0027-FY) in IF

Representative immunofluorescence images showing expression and localisation of GR (green) and p57 (red) in GR-WT and GR-KO H2122 cell lines, with DAPI as nuclear staining (blue) (n=2). Scale bar, 50 μm. ...View More

Prekovic, S., Schuurman, K., Mayayo-Peralta, I., Manjón, A. G., Buijs, M., Yavuz, S., ... & Zwart, W. (2021). Glucocorticoid receptor triggers a reversible drug-tolerant dormancy state with acquired therapeutic vulnerabilities in lung cancer. Nature Communications, 12(1), 4360.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-R0027-FY-4

Mouse Anti-CDKN1C Monoclonal Antibody (R0027) (CBMAB-R0027-FY) in IP

Co-IP followed by immunoblotting investigating binding of exogenous p57 and domains of p57 to HDAC1. HA-p57, aminoterminal (HA-p57-Nt) and carboxyterminal (HA-p57-Ct) domains were expressed in 293 cells together with FLAG-HDAC1. p57 proteins were immunoprecipitated using anti-HA antibodies and the precipitated p57 proteins were detected by using mouse anti-HA antibodies. ...View More

Kullmann, M. K., Pegka, F., Ploner, C., & Hengst, L. (2021). Stimulation of c-Jun/AP-1-activity by the cell cycle inhibitor p57Kip2. Frontiers in Cell and Developmental Biology, 9, 664609.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-R0027-FY-5

Mouse Anti-CDKN1C Monoclonal Antibody (R0027) (CBMAB-R0027-FY) in WB

Western blotting analysis of p27Kip1 and p57Kip2 expression in C2C12 myoblasts after treatment with 5 or 10 µg/mL CPP531, or 5 μM BIO, for 72 h. ...View More

Kim, A. R., Kim, S. W., Lee, B. W., Kim, K. H., Kim, W. H., Seok, H., ... & Williams, D. R. (2020). Screening ginseng saponins in progenitor cells identifies 20 (R)-ginsenoside Rh2 as an enhancer of skeletal and cardiac muscle regeneration. Scientific Reports, 10(1), 4967.

Distributed under Open Access license CC BY 4.0, without modification.

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

R0027

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Full length p57 Kip2 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

> 90% Purity determined by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin Dependent Kinase Inhibitor 1C

Introduction

CDKN1C (Cyclin Dependent Kinase Inhibitor 1C) is a Protein Coding gene. Diseases associated with CDKN1C include Beckwith-Wiedemann Syndrome and Intrauterine Growth Retardation, Metaphyseal Dysplasia, Adrenal Hypoplasia Congenita, And Genital Anomalies. Among its related pathways are Primary Focal Segmental Glomerulosclerosis FSGS and DNA Damage. Gene Ontology (GO) annotations related to this gene include cyclin-dependent protein serine/threonine kinase inhibitor activity. An important paralog of this gene is CDKN1B.

Entrez Gene ID

UniProt ID

Alternative Names

Cyclin Dependent Kinase Inhibitor 1C; Cyclin-Dependent Kinase Inhibitor 1C (P57, Kip2); Cyclin-Dependent Kinase Inhibitor P57; P57Kip2; KIP2; Cyclin-Dependent Kinase Inhibitor 1C

Function

Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.

Biological Process

Cell cycle arrest Source: InterPro
Negative regulation of cyclin-dependent protein kinase activity Source: CAFA
Negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Negative regulation of epithelial cell proliferation Source: BHF-UCL
Negative regulation of kinase activity Source: UniProtKB
Negative regulation of mitotic cell cycle Source: GO_Central
Negative regulation of phosphorylation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL

Cellular Location

Nucleus

Involvement in disease

Beckwith-Wiedemann syndrome (BWS): A disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
Intrauterine growth retardation, metaphyseal dysplasia, adrenal hypoplasia congenita, and genital anomalies (IMAGE): A rare condition characterized by intrauterine growth restriction, metaphyseal dysplasia, congenital adrenal hypoplasia, and genital anomalies. Patients with this condition may present shortly after birth with severe adrenal insufficiency, which can be life-threatening if not recognized early and commenced on steroid replacement therapy. Other reported features in this condition include, hypercalciuria and/or hypercalcemia, craniosynostosis, cleft palate, and scoliosis.

Yang, D., Feng, W., Zhuang, Y., Liu, J., Feng, Z., Xu, T., ... & Wang, Z. (2021). Long non-coding RNA linc00665 inhibits CDKN1C expression by binding to EZH2 and affects cisplatin sensitivity of NSCLC cells. Molecular Therapy-Nucleic Acids, 23, 1053-1065.

Kagiyama, Y., Fujita, S., Shima, Y., Yamagata, K., Katsumoto, T., Nakagawa, M., ... & Kitabayashi, I. (2021). CDKN1C‐mediated growth inhibition by an EZH1/2 dual inhibitor overcomes resistance of mantle cell lymphoma to ibrutinib. Cancer Science, 112(6), 2314.

Yang, C., Yan, Z., Hu, F., Wei, W., Sun, Z., & Xu, W. (2020). Silencing of microRNA-517a induces oxidative stress injury in melanoma cells via inactivation of the JNK signaling pathway by upregulating CDKN1C. Cancer cell international, 20(1), 1-14.

Na, H., Li, X., Zhang, X., Xu, Y., Sun, Y., Cui, J., ... & Zuo, Y. (2020). lncRNA STEAP3-AS1 Modulates Cell Cycle Progression via Affecting CDKN1C Expression through STEAP3 in Colon Cancer. Molecular Therapy-Nucleic Acids, 21, 480-491.

Wang, Z., Zhu, H., Shi, H., Zhao, H., Gao, R., Weng, X., ... & Ge, J. (2019). Exosomes derived from M1 macrophages aggravate neointimal hyperplasia following carotid artery injuries in mice through miR-222/CDKN1B/CDKN1C pathway. Cell death & disease, 10(6), 1-15.

Lalem, T., Zhang, L., Scholz, M., Burkhardt, R., Saccheti, V., Teren, A., ... & Devaux, Y. (2019). Cyclin dependent kinase inhibitor 1 C is a female-specific marker of left ventricular function after acute myocardial infarction. International journal of cardiology, 274, 319-325.

Qiu, Z., Zhu, W., Meng, H., Tong, L., Li, X., Luo, P., ... & Zhang, J. (2019). CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics, 9(16), 4717.

Suntharalingham, J. P., Ishida, M., Buonocore, F., Del Valle, I., Solanky, N., Demetriou, C., ... & Achermann, J. C. (2019). Analysis of CDKN1C in fetal growth restriction and pregnancy loss. F1000Research, 8.

Lalem, T., Zhang, L., Scholz, M., Burkhardt, R., Saccheti, V., Thiery, J., ... & Cardiolinc™ network (www. cardiolinc. org). (2018). P830 Cyclin dependent kinase inhibitor 1C (CDKN1C), a novel female-specific marker of left ventricular dysfunction after acute myocardial infarction. European Heart Journal, 39(suppl_1), ehy564-P830.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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