Rabbit Anti-CELSR2 Recombinant Antibody (D2M9H) (CBMAB-CP0278-LY)

The product is antibody recognizes CELSR2. The antibody D2M9H immunoassay techniques such as: WB,IHC-P.
See all CELSR2 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

D2M9H

Antibody Isotype

IgG

Application

WB, IHC-P

Basic Information

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His1781 of human CELSR2 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cadherin EGF LAG Seven-Pass G-Type Receptor 2

Introduction

The protein encoded by this gene is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like repeats and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact-mediated communication, with cadherin domains acting as homophilic binding regions and the EGF-like domains involved in cell adhesion and receptor-ligand interactions. The specific function of this particular member has not been determined. [provided by RefSeq, Jul 2008]

Entrez Gene ID

1952

UniProt ID

Q9HCU4

Alternative Names

Cadherin EGF LAG Seven-Pass G-Type Receptor 2; Multiple Epidermal Growth Factor-Like Domains Protein 3; Adhesion G Protein-Coupled Receptor C2; Epidermal Growth Factor-Like Protein 2; Multiple EGF-Like Domains Protein 3; Cadherin Family Member 10; Flamingo Homolog 3; EGF-Like Protein 2; CDHF10; EGFL2; MEGF3;

Function

Receptor that may have an important role in cell/cell signaling during nervous system formation.

Biological Process

Cell-cell adhesion Source: GO_Central
Cerebrospinal fluid secretion Source: Ensembl
Cilium assembly Source: Ensembl
Cilium movement Source: Ensembl
Dendrite morphogenesis Source: BHF-UCL
G protein-coupled receptor signaling pathway Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: BHF-UCL
Neural plate anterior/posterior regionalization Source: BHF-UCL
Neuron migration Source: Ensembl
Regulation of cell-cell adhesion Source: BHF-UCL
Regulation of protein localization Source: Ensembl
Regulation of transcription, DNA-templated Source: BHF-UCL
Ventricular system development Source: Ensembl
Wnt signaling pathway Source: BHF-UCL
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 32-2380
Helical: 2381-2401
Cytoplasmic: 2402-2416
Helical: 2417-2437
Extracellular: 2438
Helical: 2439-2459
Cytoplasmic: 2460-2480
Helical: 2481-2501
Extracellular: 2502-2519
Helical: 2520-2540
Cytoplasmic: 2541-2560
Helical: 2561-2581
Extracellular: 2582-2591
Helical: 2592-2612
Cytoplasmic: 2613-2923

PTM

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

References

Tan, J., Che, Y., Liu, Y., Hu, J., Wang, W., Hu, L., ... & Li, J. (2021). CELSR2 deficiency suppresses lipid accumulation in hepatocyte by impairing the UPR and elevating ROS level. The FASEB Journal, 35(10), e21908.

Xu, M., Zhu, S., Xu, R., & Lin, N. (2020). Identification of CELSR2 as a novel prognostic biomarker for hepatocellular carcinoma. BMC cancer, 20(1), 1-15.

Zhou, X., Zhan, Z., Tang, C., Li, J., Zheng, X., Zhu, S., & Qi, J. (2020). Silencing Celsr2 inhibits the proliferation and migration of Schwann cells through suppressing the Wnt/β-catenin signaling pathway. Biochemical and Biophysical Research Communications, 533(4), 623-630.

Einarsdottir, E., Grauers, A., Wang, J., Jiao, H., Escher, S. A., Danielsson, A., ... & Gerdhem, P. (2017). CELSR2 is a candidate susceptibility gene in idiopathic scoliosis. PloS one, 12(12), e0189591.

Goffinet, A. M., & Tissir, F. (2017, September). Seven pass cadherins CELSR1-3. In Seminars in cell & developmental biology (Vol. 69, pp. 102-110). Academic Press.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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