Mouse Anti-CGGBP1 Recombinant Antibody (CBFYC-1790) (CBMAB-C1852-FY)

Name: Mouse Anti-CGGBP1 Recombinant Antibody (CBFYC-1790)
SKU: CBMAB-C1852-FY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Pig

Clone

CBFYC-1790

Antibody Isotype

IgG3, κ

Application

ELISA, IF, IP, WB

Basic Information

Immunogen

Amino acids 77-99 within an internal region of CGGBP1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Pig

Antibody Isotype

IgG3, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 58-168

Target

Full Name

CGG triplet repeat binding protein 1

Introduction

This gene encodes a CGG repeat-binding protein that primarily localizes to the nucleus. CGG trinucleotide repeats are implicated in many disorders as they often act as transcription-and translation-regulatory elements, can produce hairpin structures which cause DNA replication errors, and form regions prone to chromosomal breakage. CGG repeats are also targets for CpG methylation. In addition to its ability to bind CGG repeats and regulate transcription, this gene is believed to play a role in DNA damage repair and telomere protection. In vitro studies indicate this protein does not bind to methylated CpG sequences.

Entrez Gene ID

8545

UniProt ID

Q9UFW8

Alternative Names

CGG Triplet Repeat Binding Protein 1; P20-CGGBP DNA-Binding Protein; 20 KDa CGG-Binding Protein; P20-CGG Binding Protein; CGG-Binding Protein 1; CGGBP; CGG Triplet Repeat-Binding Protein 1; P20-CGGBP

Function

Binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. May play a role in regulating FMR1 promoter.

Biological Process

Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of gene expression Source: GO_Central
Regulation of gene expression, epigenetic Source: ARUK-UCL

Cellular Location

Nucleus

More Infomation

References

Yellan, I., Yang, A. W., & Hughes, T. R. (2021). Diverse Eukaryotic CGG-Binding Proteins Produced by Independent Domestications of hAT Transposons. Molecular biology and evolution, 38(5), 2070-2075.

Patel, M., Patel, D., Datta, S., & Singh, U. (2020). CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity. BMC genetics, 21(1), 1-21.

Patel, M., Patel, D., Datta, S., & Singh, U. (2020). An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1. BMC cancer, 20(1), 1-14.

Patel, M., Patel, D., Datta, S., & Singh, U. (2020). HDAC inhibitor Givinostat targets DNA-binding of human CGGBP1.

Patel, D., Patel, M., Datta, S., & Singh, U. (2019). CGGBP1 regulates CTCF occupancy at repeats. Epigenetics & chromatin, 12(1), 1-22.

Patel, D., Patel, M., & Singh, U. (2019). CGGBP1 regulates chromatin barrier activity and CTCF occupancy at repeats. bioRxiv, 593137.

Patel, D., Patel, M., Westermark, B., & Singh, U. (2018). Dynamic bimodal changes in CpG and non-CpG methylation genome-wide upon CGGBP1 loss-of-function. BMC research notes, 11(1), 1-7.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CGGBP1 Recombinant Antibody (1D11)

Mouse Anti-CGGBP1 Recombinant Antibody (CBXC-0406)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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