Mouse Anti-CGN Recombinant Antibody (16k70) (CBMAB-C3388-LY)

This product is antibody recognizes CGN. The antibody 16k70 immunoassay techniques such as: IHC-F, WB.
See all CGN antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Cattle, Human, Mouse

Clone

16k70

Antibody Isotype

IgG

Application

IHC-F, WB

Basic Information

Immunogen

Synthetic peptide corresponding to residues 4-24 of cingulin

Specificity

Cattle, Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

CINGULIN

Introduction

CGN (Cingulin) is a Protein Coding gene. Among its related pathways are Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways and Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer. Gene Ontology (GO) annotations related to this gene include actin binding and motor activity. An important paralog of this gene is CGNL1.

Entrez Gene ID

Human	57530
Mouse	70737
Cattle	533725

UniProt ID

Human	Q9P2M7
Mouse	P59242
Cattle	P23805

Function

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Biological Process

Transforming growth factor beta receptor signaling pathway Source: Reactome

Cellular Location

Tight junction. Localizes to the apical junction complex composed of tight and adherens junctions (By similarity). Colocalizes with SPEF1 at sites of cell-cell contact in intestinal epithelial cells (PubMed:12023291).

References

Holzner, S., Bromberger, S., Wenzina, J., Neumüller, K., Holper, T. M., Petzelbauer, P., ... & Schossleitner, K. (2021). Phosphorylated cingulin localises GEF-H1 at tight junctions to protect vascular barriers in blood endothelial cells. Journal of cell science, 134(17), jcs258557.

Liu, Y., Guo, W., Fang, S., He, B., Li, X., & Fan, L. (2021). miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin. European Journal of Histochemistry, 65(4).

Zhang, X., Li, T., Han, Y. N., Ge, M., Wang, P., Sun, L., ... & Lu, Y. (2021). miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN. Cancers, 13(22), 5710.

Zhuravleva, K., Goertz, O., Wölkart, G., Guillemot, L., Petzelbauer, P., Lehnhardt, M., ... & Schossleitner, K. (2020). The tight junction protein cingulin regulates the vascular response to burn injury in a mouse model. Microvascular Research, 132, 104067.

Vasileva, E., Rouaud, F., Spadaro, D., Huang, W., Colom, A., Flinois, A., ... & Citi, S. (2020). Cingulin unfolds ZO-1 and organizes myosin-2B and γ-actin to mechanoregulate apical and tight junction membranes. BioRXiv.

Martínez, C., Rodiño-Janeiro, B. K., Lobo, B., Stanifer, M. L., Klaus, B., Granzow, M., ... & Santos, J. (2017). miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea. Gut, 66(9), 1537-1538.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-CGN (AA 4-24) Recombinant Antibody (CBFYC-1791)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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