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Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY)


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Published Data
CBMAB-C1853-FY-1
Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IF
Double knockout cells were transfected with GFP-ZO-1 or GFP-ZO-2 and TJ formation was analysed by immunostaining occludin and cingulin. ...View More
Haas, A. J., Zihni, C., Krug, S. M., Maraspini, R., Otani, T., Furuse, M., ... & Matter, K. (2022). ZO-1 guides tight junction assembly and epithelial morphogenesis via cytoskeletal tension-dependent and-independent functions. Cells, 11(23), 3775.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1853-FY-2
Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IF
Indirect immunofluorescence of adult (P60) mouse liver cryosections with anti-Cx26 antibody (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is in a white pseudo color, according to DAPI staining. The analysis was performed at confocal microscopy with z-sections of 0.5 µm (LSM880, Carl Zeiss, Oberkochen, Germany). Each image consists of the maximum intensity projection of all z-sections obtained. Arrowheads: signal at the plasma membrane. Arrows: merged signals. Scale bar: 10 µm. ...View More
Batissoco, A. C., Salazar-Silva, R., Oiticica, J., Bento, R. F., Mingroni-Netto, R. C., & Haddad, L. A. (2018). A cell junctional protein network associated with connexin-26. International journal of molecular sciences, 19(9), 2535.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1853-FY-3
Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IP
Immunoprecipitation of cingulin from adult mouse liver and its co-immunoprecipitation with CX26, CX30, CX31, CX43, EB2, TJP1, and ASS1, which is indicated by the respective arrows. No co-immunoprecipitation is observed with VCL. The protein molecular mass is indicated in kiloDaltons (kDa). ...View More
Batissoco, A. C., Salazar-Silva, R., Oiticica, J., Bento, R. F., Mingroni-Netto, R. C., & Haddad, L. A. (2018). A cell junctional protein network associated with connexin-26. International journal of molecular sciences, 19(9), 2535.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1853-FY-4
Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in WB
Measurements of TER were performed in unstimulated ECs over time. siRNA-induced cingulin protein depletion was confirmed by Western blotting. ...View More
Tian, Y., Gawlak, G., Tian, X., Shah, A. S., Sarich, N., Citi, S., & Birukova, A. A. (2016). Role of cingulin in agonist-induced vascular endothelial permeability. Journal of Biological Chemistry, 291(45), 23681-23692.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1853-FY-5
Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IF
Cells grown on glass coverslips with immobilized biotinylated gelatin (0.25 mg/ml) were stimulated with vehicle (Veh) or thrombin (Thr) for 10 min followed by addition of FITC-avidin (25 μg/ml, 3 min). Unbound FITC-avidin was removed, and FITC fluorescence signal was visualized by fluorescence microscopy; bar, 5 μm. ...View More
Tian, Y., Gawlak, G., Tian, X., Shah, A. S., Sarich, N., Citi, S., & Birukova, A. A. (2016). Role of cingulin in agonist-induced vascular endothelial permeability. Journal of Biological Chemistry, 291(45), 23681-23692.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Dog, Human
Clone
CBFYC-1791
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 821-1000 mapping near the C-terminus of Cingulin of human.
Host Species
Mouse
Specificity
Dog, Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 4-24

Target

Full Name
CINGULIN
Introduction
CGN (Cingulin) is a Protein Coding gene. Among its related pathways are Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways and Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer. Gene Ontology (GO) annotations related to this gene include actin binding and motor activity. An important paralog of this gene is CGNL1.
Entrez Gene ID
Human57530
Mouse70737
Cattle533725
Frog100145755
UniProt ID
HumanQ9P2M7
MouseP59242
CattleP23805
FrogQ9PTD7
Alternative Names
Cingulin; KIAA1319
Function
Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Biological Process
Transforming growth factor beta receptor signaling pathway Source: Reactome
Cellular Location
Tight junction. Localizes to the apical junction complex composed of tight and adherens junctions (By similarity). Colocalizes with SPEF1 at sites of cell-cell contact in intestinal epithelial cells (PubMed:12023291).
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For research use only. Not intended for any clinical use.

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