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Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY)

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Published Data

CBMAB-C1853-FY-1

Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IF

Double knockout cells were transfected with GFP-ZO-1 or GFP-ZO-2 and TJ formation was analysed by immunostaining occludin and cingulin. ...View More

Haas, A. J., Zihni, C., Krug, S. M., Maraspini, R., Otani, T., Furuse, M., ... & Matter, K. (2022). ZO-1 guides tight junction assembly and epithelial morphogenesis via cytoskeletal tension-dependent and-independent functions. Cells, 11(23), 3775.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C1853-FY-2

Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IF

Indirect immunofluorescence of adult (P60) mouse liver cryosections with anti-Cx26 antibody (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is in a white pseudo color, according to DAPI staining. The analysis was performed at confocal microscopy with z-sections of 0.5 µm (LSM880, Carl Zeiss, Oberkochen, Germany). Each image consists of the maximum intensity projection of all z-sections obtained. Arrowheads: signal at the plasma membrane. Arrows: merged signals. Scale bar: 10 µm. ...View More

Batissoco, A. C., Salazar-Silva, R., Oiticica, J., Bento, R. F., Mingroni-Netto, R. C., & Haddad, L. A. (2018). A cell junctional protein network associated with connexin-26. International journal of molecular sciences, 19(9), 2535.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C1853-FY-3

Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IP

Immunoprecipitation of cingulin from adult mouse liver and its co-immunoprecipitation with CX26, CX30, CX31, CX43, EB2, TJP1, and ASS1, which is indicated by the respective arrows. No co-immunoprecipitation is observed with VCL. The protein molecular mass is indicated in kiloDaltons (kDa). ...View More

Batissoco, A. C., Salazar-Silva, R., Oiticica, J., Bento, R. F., Mingroni-Netto, R. C., & Haddad, L. A. (2018). A cell junctional protein network associated with connexin-26. International journal of molecular sciences, 19(9), 2535.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C1853-FY-4

Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in WB

Measurements of TER were performed in unstimulated ECs over time. siRNA-induced cingulin protein depletion was confirmed by Western blotting. ...View More

Tian, Y., Gawlak, G., Tian, X., Shah, A. S., Sarich, N., Citi, S., & Birukova, A. A. (2016). Role of cingulin in agonist-induced vascular endothelial permeability. Journal of Biological Chemistry, 291(45), 23681-23692.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C1853-FY-5

Mouse Anti-CGN Recombinant Antibody (CBFYC-1791) (CBMAB-C1853-FY) in IF

Cells grown on glass coverslips with immobilized biotinylated gelatin (0.25 mg/ml) were stimulated with vehicle (Veh) or thrombin (Thr) for 10 min followed by addition of FITC-avidin (25 μg/ml, 3 min). Unbound FITC-avidin was removed, and FITC fluorescence signal was visualized by fluorescence microscopy; bar, 5 μm. ...View More

Tian, Y., Gawlak, G., Tian, X., Shah, A. S., Sarich, N., Citi, S., & Birukova, A. A. (2016). Role of cingulin in agonist-induced vascular endothelial permeability. Journal of Biological Chemistry, 291(45), 23681-23692.

Distributed under Open Access license CC BY 4.0, without modification.

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1791

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 821-1000 mapping near the C-terminus of Cingulin of human.

Host Species

Mouse

Specificity

Dog, Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 4-24

More Infomation

Target

Full Name

CINGULIN

Introduction

CGN (Cingulin) is a Protein Coding gene. Among its related pathways are Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways and Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer. Gene Ontology (GO) annotations related to this gene include actin binding and motor activity. An important paralog of this gene is CGNL1.

Entrez Gene ID

Human	57530
Mouse	70737
Cattle	533725
Frog	100145755

UniProt ID

Human	Q9P2M7
Mouse	P59242
Cattle	P23805
Frog	Q9PTD7

Alternative Names

Cingulin; KIAA1319

Function

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Biological Process

Transforming growth factor beta receptor signaling pathway Source: Reactome

Cellular Location

Tight junction. Localizes to the apical junction complex composed of tight and adherens junctions (By similarity). Colocalizes with SPEF1 at sites of cell-cell contact in intestinal epithelial cells (PubMed:12023291).

Holzner, S., Bromberger, S., Wenzina, J., Neumüller, K., Holper, T. M., Petzelbauer, P., ... & Schossleitner, K. (2021). Phosphorylated cingulin localises GEF-H1 at tight junctions to protect vascular barriers in blood endothelial cells. Journal of cell science, 134(17), jcs258557.

Liu, Y., Guo, W., Fang, S., He, B., Li, X., & Fan, L. (2021). miR-1270 enhances the proliferation, migration, and invasion of osteosarcoma via targeting cingulin. European Journal of Histochemistry, 65(4).

Zhang, X., Li, T., Han, Y. N., Ge, M., Wang, P., Sun, L., ... & Lu, Y. (2021). miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN. Cancers, 13(22), 5710.

Zhuravleva, K., Goertz, O., Wölkart, G., Guillemot, L., Petzelbauer, P., Lehnhardt, M., ... & Schossleitner, K. (2020). The tight junction protein cingulin regulates the vascular response to burn injury in a mouse model. Microvascular Research, 132, 104067.

Vasileva, E., Rouaud, F., Spadaro, D., Huang, W., Colom, A., Flinois, A., ... & Citi, S. (2020). Cingulin unfolds ZO-1 and organizes myosin-2B and γ-actin to mechanoregulate apical and tight junction membranes. BioRXiv.

Martínez, C., Rodiño-Janeiro, B. K., Lobo, B., Stanifer, M. L., Klaus, B., Granzow, M., ... & Santos, J. (2017). miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea. Gut, 66(9), 1537-1538.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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