Mouse Anti-CHD1L Recombinant Antibody (2170C3a) (CBMAB-C0638-LY)

Name: Mouse Anti-CHD1L Recombinant Antibody (2170C3a)
SKU: CBMAB-C0638-LY
Rating: 5 (1 reviews)

See all CHD1L antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2170C3a

Application

IF, IP, WB

Immunogen

Recombinant protein corresponding to a region near the C-terminus of CHD1L of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chromodomain Helicase DNA Binding Protein 1 Like

Introduction

This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Overexpression of this gene has been linked to several types of cancers. [provided by RefSeq, Feb 2017]

Entrez Gene ID

9557

UniProt ID

Q86WJ1

Alternative Names

Chromodomain Helicase DNA Binding Protein 1 Like; Amplified In Liver Cancer Protein 1; Amplified In Liver Cancer 1; ALC1; Chromodomain-Helicase-DNA-Binding Protein 1-Like; EC 3.6.4.12; EC 3.6.1; CHDL;

Function

DNA helicase which plays a role in chromatin-remodeling following DNA damage (PubMed:19661379, PubMed:29220653).
Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair (PubMed:19661379).
Able to catalyze nucleosome sliding in an ATP-dependent manner (PubMed:19661379).
Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding (PubMed:19661379, PubMed:29220653).

Biological Process

Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin remodeling Source: UniProtKB
DNA repair Source: UniProtKB
Global genome nucleotide-excision repair Source: Reactome
Nucleotide-excision repair, DNA duplex unwinding Source: Reactome
Nucleotide-excision repair, DNA incision Source: Reactome
Nucleotide-excision repair, DNA incision, 3'-to lesion Source: Reactome
Nucleotide-excision repair, DNA incision, 5'-to lesion Source: Reactome
Nucleotide-excision repair, preincision complex assembly Source: Reactome
Nucleotide-excision repair, preincision complex stabilization Source: Reactome

Cellular Location

Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.

Zhang, X., & Sun, Y. (2022). Chromodomain Helicase DNA Binding Protein 1-like, a negative regulator of Forkhead box O3a, promotes the proliferation and migration of Angiotensin II-induced vascular smooth muscle cells. Bioengineered, 13(2), 2597-2609.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CHD1L Recombinant Antibody (2170C3a)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-CHD1L Recombinant Antibody (CBWJC-2630) (CAT#: CBMAB-C3715WJ)

Rabbit Anti-CHD1L Recombinant Antibody (CBFYC-1810) (CAT#: CBMAB-C1872-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry