Mouse Anti-CHD4 Recombinant Antibody (CB472A) (CBMAB-CA472LY)

Name: Mouse Anti-CHD4 Recombinant Antibody (CB472A)
SKU: CBMAB-CA472LY
Rating: 5 (1 reviews)

See all CHD4 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CB472A

Application

ELISA, IF, IP, WB, IHC-P

Immunogen

Amino acids 100-121 near the N-terminus of Mi2-β of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Pig, Horse, Cattle

Antibody Isotype

IgG3, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chromodomain Helicase DNA Binding Protein 4

Introduction

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Entrez Gene ID

1108

UniProt ID

Q14839

Alternative Names

Chromodomain Helicase DNA Binding Protein 4; Mi-2 Autoantigen 218 KDa Protein; ATP-Dependent Helicase CHD4; Mi2-BETA; CHD-4; Chromodomain-Helicase-DNA-Binding Protein 4;

Function

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

Biological Process

ATP-dependent chromatin remodeling Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: Reactome
Regulation of transcription by RNA polymerase II Source: ProtInc

Cellular Location

Centrosome; Nucleus. Associates with centrosomes in interphase.

Involvement in disease

Sifrim-Hitz-Weiss syndrome (SIHIWES): An autosomal dominant syndrome characterized by mental retardation, variable congenital defects affecting cardiac, skeletal, and urogenital systems. Short stature, macrocephaly, hearing impairment, and facial dysmorphism are present in some patients.

Novillo, A., Fernández-Santander, A., Gaibar, M., Galán, M., Romero-Lorca, A., El Abdellaoui-Soussi, F., & Gómez-del Arco, P. (2021). Role of Chromodomain-Helicase-DNA-Binding Protein 4 (CHD4) in Breast Cancer. Frontiers in Oncology, 11.

Hagman, J. R., Arends, T., Laborda, C., Knapp, J. R., Harmacek, L., & O’Connor, B. P. (2021). Chromodomain helicase DNA‐binding 4 (CHD4) regulates early B cell identity and V (D) J recombination. Immunological reviews.

Novillo, A. (2021). Ferna ndez-Santander A, Gaibar M, Gala n M, Romero-Lorca A, El Abdellaoui-Soussi F and Go mez-del Arco P (2021) Role of Chromodomain-Helicase-DNA-Binding Protein 4 (CHD4) in Breast Cancer. Front. Oncol, 11, 633233.

Sakaguchi, C., Ichihara, K., Nita, A., Katayama, Y., & Nakayama, K. I. (2021). Identification and characterization of novel proteins associated with CHD4. Genes to Cells.

Xu, N., Liu, F., Wu, S., Ye, M., Ge, H., Zhang, M., ... & Bai, C. (2020). CHD4 mediates proliferation and migration of non-small cell lung cancer via the RhoA/ROCK pathway by regulating PHF5A. BMC cancer, 20(1), 1-11.

Zhang, J., Shih, D. J., & Lin, S. Y. (2019). The Tale of CHD4 in DNA Damage Response and Chemotherapeutic Response. Journal of cancer research and cellular therapeutics, 3(1).

Ou-Yang, F., Pan, M. R., Chang, S. J., Wu, C. C., Fang, S. Y., Li, C. L., ... & Luo, C. W. (2019). Identification of CHD4-β1 integrin axis as a prognostic marker in triple-negative breast cancer using next-generation sequencing and bioinformatics. Life sciences, 238, 116963.

Arends, T., Dege, C., Bortnick, A., Danhorn, T., Knapp, J. R., Jia, H., ... & Hagman, J. R. (2019). CHD4 is essential for transcriptional repression and lineage progression in B lymphopoiesis. Proceedings of the National Academy of Sciences, 116(22), 10927-10936.

D'Alesio, C., Bellese, G., Gagliani, M. C., Lechiara, A., Dameri, M., Grasselli, E., ... & Castagnola, P. (2019). The chromodomain helicase CHD4 regulates ERBB2 signaling pathway and autophagy in ERBB2+ breast cancer cells. Biology open, 8(4), bio038323.

Luo, C. W., Wu, C. C., Chang, S. J., Chang, T. M., Chen, T. Y., Chai, C. Y., ... & Pan, M. R. (2018). CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells. Experimental cell research, 363(1), 65-72.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CHD4 Recombinant Antibody (CB472A)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CHD4 Recombinant Antibody (CBXC-2846) (CAT#: CBMAB-C5387-CQ)

Mouse Anti-CHD4 Recombinant Antibody (CBT3590) (CAT#: V2LY-0625-LY1265)

Rabbit Anti-CHD4 Recombinant Antibody (CBWJC-2635) (CAT#: CBMAB-C3720WJ)

Mouse Anti-CHD4 Recombinant Antibody (CAP497) (CAT#: CBMAB-AP1492LY)

Mouse Anti-CHD4 Recombinant Antibody (4H4) (CAT#: CBMAB-C7053-LY)

Mouse Anti-CHD4 Recombinant Antibody (CAP496) (CAT#: CBMAB-AP1491LY)

Mouse Anti-CHD4 Recombinant Antibody (CBWJC-2029) (CAT#: CBMAB-C2645WJ)

Mouse Anti-CHD4 Recombinant Antibody (5F1-A11-C9) (CAT#: CBMAB-C7557-LY)

Mouse Anti-CHD4 Recombinant Antibody (CBFYC-1812) (CAT#: CBMAB-C1874-FY)

Mouse Anti-CHD4 Recombinant Antibody (CF097) (CAT#: CBMAB-FT375LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry