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Mouse Anti-CHD4 Recombinant Antibody (CBWJC-2029) (CBMAB-C2645WJ)

This product is a Mouse antibody that recognizes CHD4. This antibody CBWJC-2029 can be used for immunoassay techniques such as: WB, IP, FC, ELISA, IHC, ICC, IP, CyTOF.
See all CHD4 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBWJC-2029
Antibody Isotype
IgG2a
Application
WB, IP, FC, ELISA, IHC, ICC, IP, CyTOF

Basic Information

Immunogen
Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Chromodomain Helicase DNA Binding Protein 4
Introduction
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Entrez Gene ID
Human1108
Mouse107932
UniProt ID
HumanQ14839
MouseQ6PDQ2
Alternative Names
Chromodomain Helicase DNA Binding Protein 4; Mi-2 Autoantigen 218 KDa Protein; ATP-Dependent Helicase CHD4; Mi2-BETA; CHD-4; Chromodomain-Helicase-DNA-Binding Protein 4;
Function
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Biological Process
ATP-dependent chromatin remodeling Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: Reactome
Regulation of transcription by RNA polymerase II Source: ProtInc
Cellular Location
Centrosome; Nucleus. Associates with centrosomes in interphase.
Involvement in disease
Sifrim-Hitz-Weiss syndrome (SIHIWES): An autosomal dominant syndrome characterized by mental retardation, variable congenital defects affecting cardiac, skeletal, and urogenital systems. Short stature, macrocephaly, hearing impairment, and facial dysmorphism are present in some patients.

Novillo, A., Fernández-Santander, A., Gaibar, M., Galán, M., Romero-Lorca, A., El Abdellaoui-Soussi, F., & Gómez-del Arco, P. (2021). Role of Chromodomain-Helicase-DNA-Binding Protein 4 (CHD4) in Breast Cancer. Frontiers in Oncology, 11.

Hagman, J. R., Arends, T., Laborda, C., Knapp, J. R., Harmacek, L., & O’Connor, B. P. (2021). Chromodomain helicase DNA‐binding 4 (CHD4) regulates early B cell identity and V (D) J recombination. Immunological reviews.

Novillo, A. (2021). Ferna ndez-Santander A, Gaibar M, Gala n M, Romero-Lorca A, El Abdellaoui-Soussi F and Go mez-del Arco P (2021) Role of Chromodomain-Helicase-DNA-Binding Protein 4 (CHD4) in Breast Cancer. Front. Oncol, 11, 633233.

Sakaguchi, C., Ichihara, K., Nita, A., Katayama, Y., & Nakayama, K. I. (2021). Identification and characterization of novel proteins associated with CHD4. Genes to Cells.

Xu, N., Liu, F., Wu, S., Ye, M., Ge, H., Zhang, M., ... & Bai, C. (2020). CHD4 mediates proliferation and migration of non-small cell lung cancer via the RhoA/ROCK pathway by regulating PHF5A. BMC cancer, 20(1), 1-11.

Zhang, J., Shih, D. J., & Lin, S. Y. (2019). The Tale of CHD4 in DNA Damage Response and Chemotherapeutic Response. Journal of cancer research and cellular therapeutics, 3(1).

Ou-Yang, F., Pan, M. R., Chang, S. J., Wu, C. C., Fang, S. Y., Li, C. L., ... & Luo, C. W. (2019). Identification of CHD4-β1 integrin axis as a prognostic marker in triple-negative breast cancer using next-generation sequencing and bioinformatics. Life sciences, 238, 116963.

Arends, T., Dege, C., Bortnick, A., Danhorn, T., Knapp, J. R., Jia, H., ... & Hagman, J. R. (2019). CHD4 is essential for transcriptional repression and lineage progression in B lymphopoiesis. Proceedings of the National Academy of Sciences, 116(22), 10927-10936.

D'Alesio, C., Bellese, G., Gagliani, M. C., Lechiara, A., Dameri, M., Grasselli, E., ... & Castagnola, P. (2019). The chromodomain helicase CHD4 regulates ERBB2 signaling pathway and autophagy in ERBB2+ breast cancer cells. Biology open, 8(4), bio038323.

Luo, C. W., Wu, C. C., Chang, S. J., Chang, T. M., Chen, T. Y., Chai, C. Y., ... & Pan, M. R. (2018). CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells. Experimental cell research, 363(1), 65-72.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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