Mouse Anti-CHKA Recombinant Antibody (1063) (CBMAB-1063CQ)

Name: Mouse Anti-CHKA Recombinant Antibody (1063)
SKU: CBMAB-1063CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1063

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 23-61 near the N-terminus of ChoK of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CHKA

Introduction

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Diseases associated with CHKA include Small Cell Carcinoma Of The Bladder and Hepatitis C Virus. Among its related pathways are Metabolism and phosphatidylcholine biosynthesis.

Entrez Gene ID

1119

UniProt ID

P35790

Alternative Names

CK; EK; CHK; CKI

Function

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.

Biological Process

Choline metabolic process Source: Ensembl
Ethanolamine metabolic process Source: Ensembl
Lipid metabolic process Source: ProtInc
Lipid transport Source: ProtInc
Phosphatidylcholine biosynthetic process Source: UniProtKB
Phosphatidylethanolamine biosynthetic process Source: UniProtKB
Response to 3-methylcholanthrene Source: Ensembl
Response to toxic substance Source: Ensembl

Cellular Location

Cytoplasm

Wang, N., Brickute, D., Braga, M., Barnes, C., Lu, H., Allott, L., & Aboagye, E. O. (2021). Novel Non-Congeneric Derivatives of the Choline Kinase Alpha Inhibitor ICL-CCIC-0019. Pharmaceutics, 13(7), 1078.

Zou, Y., Huang, L., Sun, S., Yue, F., Li, Z., Ma, Y., & Ma, H. (2021). Choline Kinase Alpha Promoted Glioma Development by Activating PI3K/AKT Signaling Pathway. Cancer Biotherapy and Radiopharmaceuticals.

Raikundalia, S., Mohamed Sa'dom, S. A. F., Few, L. L., & See Too, W. C. (2021). MicroRNA‑367‑3p induces apoptosis and suppresses migration of MCF‑7 cells by downregulating the expression of human choline kinase α. Oncology letters, 21(3), 1-1.

Mohamed Sa’dom, S. A. F., Raikundalia, S., Shamsuddin, S., See Too, W. C., & Few, L. L. (2021). DNA Methylation of Human Choline Kinase Alpha Promoter-Associated CpG Islands in MCF-7 Cells. Genes, 12(6), 853.

Kong, M., Dong, W., Xu, H., Fan, Z., Miao, X., Guo, Y., ... & Xu, Y. (2021). Choline kinase alpha is a novel transcriptional target of the Brg1 in hepatocyte: implication in liver regeneration. Frontiers in Cell and Developmental Biology, 2197.

Rizzo, A., Satta, A., Garrone, G., Cavalleri, A., Napoli, A., Raspagliesi, F., ... & Bagnoli, M. (2021). Choline kinase alpha impairment overcomes TRAIL resistance in ovarian cancer cells. Journal of Experimental & Clinical Cancer Research, 40(1), 1-13.

Yue, F., Zou, Y., Sun, S., Wang, Z., Huang, L., & Ma, H. (2020). Knockdown of choline kinase α (CHKA) inhibits the proliferation, invasion and migration of human U87MG glioma cells. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 36(8), 724-728.

Domizi, P., Malizia, F., Chazarreta-Cifre, L., Diacovich, L., & Banchio, C. (2019). KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells. Plos one, 14(1), e0210207.

Lin, X. M., Hu, L., Gu, J., Wang, R. Y., Li, L., Tang, J., ... & Chen, L. (2017). Choline kinase α mediates interactions between the epidermal growth factor receptor and mechanistic target of rapamycin complex 2 in hepatocellular carcinoma cells to promote drug resistance and xenograft tumor progression. Gastroenterology, 152(5), 1187-1202.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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