Mouse Anti-CHML Recombinant Antibody (EG676) (CBMAB-EN817-LY)

Name: Mouse Anti-CHML Recombinant Antibody (EG676)
SKU: CBMAB-EN817-LY
Rating: 5 (1 reviews)

See all CHML antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG676

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 58-97 mapping near the N-terminus of REP-2 of mouse.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CHM Like, Rab Escort Protein 2

Introduction

The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008]

Entrez Gene ID

1122

UniProt ID

P26374

Alternative Names

CHM Like, Rab Escort Protein 2; Choroideremia-Like (Rab Escort Protein 2); Choroideremia-Like Protein; REP2; Rab Proteins Geranylgeranyltransferase Component A 2; Choroideraemia-Like Protein; Rab Escort Protein 2; REP-2;

Function

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.

Biological Process

Intracellular protein transport Source: InterPro
Positive regulation of GTPase activity Source: GOC
Post-translational protein modification Source: Reactome
Protein geranylgeranylation Source: UniProtKB
Small GTPase mediated signal transduction Source: InterPro
Vesicle-mediated transport Source: GO_Central

Cellular Location

Cytosol

Chen, T. W., Yin, F. F., Yuan, Y. M., Guan, D. X., Zhang, E., Zhang, F. K., ... & Xie, D. (2019). CHML promotes liver cancer metastasis by facilitating Rab14 recycle. Nature communications, 10(1), 1-15.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CHML Recombinant Antibody (EG676)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CHML Matched Antibody Pair (269) (CAT#: APMAB-269LY)

Related Products

CHML Matched Antibody Pair (269) (CAT#: APMAB-269LY)

Mouse Anti-CHML Recombinant Antibody (5G4) (CAT#: CBMAB-C7678-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry