Mouse Anti-CHRM3 Recombinant Antibody (CBFYC-1855) (CBMAB-C1919-FY)

Name: Mouse Anti-CHRM3 Recombinant Antibody (CBFYC-1855)
SKU: CBMAB-C1919-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1855

Application

WB, ELISA, FC

Immunogen

Purified recombinant fragment of human CHRM3 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CHOLINERGIC RECEPTOR MUSCARINIC 3

Introduction

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.

Entrez Gene ID

1131

UniProt ID

P20309

Alternative Names

Cholinergic Receptor Muscarinic 3; Acetylcholine Receptor, Muscarinic 3; Muscarinic Acetylcholine Receptor M3; M3 Muscarinic Receptor; EGBRS; HM3; PBS

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Biological Process

Acetylcholine receptor signaling pathway Source: ARUK-UCL
Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Calcium-mediated signaling Source: ARUK-UCL
Cellular protein modification process Source: ProtInc
Chemical synaptic transmission Source: GO_Central
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Nervous system development Source: ProtInc
Positive regulation of smooth muscle contraction Source: InterPro
Regulation of ion transmembrane transporter activity Source: ARUK-UCL
Regulation of vascular associated smooth muscle contraction Source: GO_Central
Saliva secretion Source: InterPro
Signal transduction Source: ProtInc

Cellular Location

Endoplasmic reticulum membrane; Cell membrane; Postsynaptic cell membrane; Basolateral cell membrane. Colocalizes with TMEM147 in the endoplasmic reticulum (ER) membrane. TMEM147 impairs its trafficking to the cell membrane leading to its retention in the ER membrane.

Involvement in disease

Prune belly syndrome (PBS): A syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.

Topology

Extracellular: 1-67
Helical: 68-91
Cytoplasmic: 92-104
Helical: 105-130
Extracellular: 131-142
Helical: 143-164
Cytoplasmic: 165-184
Helical: 185-206
Extracellular: 207-229
Helical: 230-252
Cytoplasmic: 253-491
Helical: 492-514
Extracellular: 515-526
Helical: 527-546
Cytoplasmic: 547-590

Wan, J., Wang, J., & Wagner, L. E. (2021). Pancreas-specific CHRM3 activation causes pancreatitis in mice. JCI insight, 6(17).

Chen, J., Shin, V. Y., Cheuk, I., Siu, J., & Kwong, A. (2020). Abstract P1-07-01: Cholinergic receptor muscarinic 3 (CHRM3) contributes to breast cancer tumorigenesis through angiogenesis regulation.

Deng, A. Y., Huot-Marchard, J. É., deBlois, D., Thorin, E., Chauvet, C., & Menard, A. (2019). Functional dosage of muscarinic cholinergic receptor 3 signalling, not the gene dose, determines its hypertension pathogenesis. Canadian Journal of Cardiology, 35(5), 661-670.

Chee, L. Y., & Cumming, A. (2018). Polymorphisms in the cholinergic receptors muscarinic (CHRM2 and CHRM3) genes and Alzheimer’s disease. Avicenna journal of medical biotechnology, 10(3), 196.

Cowley Jr, A. W. (2018). Chrm3 Gene and M3 Muscarinic Receptors Contribute to Salt-Sensitive Hypertension: But Now a Physiological Puzzle. Hypertension, 72(3), 588-591.

Niwa, Y., Kanda, G. N., Yamada, R. G., Shi, S., Sunagawa, G. A., Ukai-Tadenuma, M., ... & Ueda, H. R. (2018). Muscarinic acetylcholine receptors Chrm1 and Chrm3 are essential for REM sleep. Cell Reports, 24(9), 2231-2247.

Deng, A. Y., deBlois, D., Laporte, S. A., Gelinas, D., Tardif, J. C., Thorin, E., ... & Ménard, A. (2018). Novel pathogenesis of hypertension and diastolic dysfunction caused by M3R (muscarinic cholinergic 3 receptor) signaling. Hypertension, 72(3), 755-764.

Patel, K. R., Bai, Y., Trieu, K. G., Barrios, J., & Ai, X. (2017). Targeting acetylcholine receptor M3 prevents the progression of airway hyperreactivity in a mouse model of childhood asthma. The FASEB Journal, 31(10), 4335-4346.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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