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Mouse Anti-CHRM3 Recombinant Antibody (CBFYC-1855) (CBMAB-C1919-FY)

This product is mouse antibody that recognizes CHRM3. The antibody CBFYC-1855 can be used for immunoassay techniques such as: IHC.
See all CHRM3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1855
Antibody Isotype
IgG2b
Application
IHC

Basic Information

Immunogen
Recombinant corresponding to human CHRM3 expressed in NSO cells (P20309)
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CHOLINERGIC RECEPTOR MUSCARINIC 3
Introduction
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
Entrez Gene ID
1131
UniProt ID
P20309
Alternative Names
Cholinergic Receptor Muscarinic 3; Acetylcholine Receptor, Muscarinic 3; Muscarinic Acetylcholine Receptor M3; M3 Muscarinic Receptor; EGBRS; HM3; PBS
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Biological Process
Acetylcholine receptor signaling pathway Source: ARUK-UCL
Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Calcium-mediated signaling Source: ARUK-UCL
Cellular protein modification process Source: ProtInc
Chemical synaptic transmission Source: GO_Central
G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Nervous system development Source: ProtInc
Positive regulation of smooth muscle contraction Source: InterPro
Regulation of ion transmembrane transporter activity Source: ARUK-UCL
Regulation of vascular associated smooth muscle contraction Source: GO_Central
Saliva secretion Source: InterPro
Signal transduction Source: ProtInc
Cellular Location
Endoplasmic reticulum membrane; Cell membrane; Postsynaptic cell membrane; Basolateral cell membrane. Colocalizes with TMEM147 in the endoplasmic reticulum (ER) membrane. TMEM147 impairs its trafficking to the cell membrane leading to its retention in the ER membrane.
Involvement in disease
Prune belly syndrome (PBS): A syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.
Topology
Extracellular: 1-67
Helical: 68-91
Cytoplasmic: 92-104
Helical: 105-130
Extracellular: 131-142
Helical: 143-164
Cytoplasmic: 165-184
Helical: 185-206
Extracellular: 207-229
Helical: 230-252
Cytoplasmic: 253-491
Helical: 492-514
Extracellular: 515-526
Helical: 527-546
Cytoplasmic: 547-590

Wan, J., Wang, J., & Wagner, L. E. (2021). Pancreas-specific CHRM3 activation causes pancreatitis in mice. JCI insight, 6(17).

Chen, J., Shin, V. Y., Cheuk, I., Siu, J., & Kwong, A. (2020). Abstract P1-07-01: Cholinergic receptor muscarinic 3 (CHRM3) contributes to breast cancer tumorigenesis through angiogenesis regulation.

Deng, A. Y., Huot-Marchard, J. É., deBlois, D., Thorin, E., Chauvet, C., & Menard, A. (2019). Functional dosage of muscarinic cholinergic receptor 3 signalling, not the gene dose, determines its hypertension pathogenesis. Canadian Journal of Cardiology, 35(5), 661-670.

Chee, L. Y., & Cumming, A. (2018). Polymorphisms in the cholinergic receptors muscarinic (CHRM2 and CHRM3) genes and Alzheimer’s disease. Avicenna journal of medical biotechnology, 10(3), 196.

Cowley Jr, A. W. (2018). Chrm3 Gene and M3 Muscarinic Receptors Contribute to Salt-Sensitive Hypertension: But Now a Physiological Puzzle. Hypertension, 72(3), 588-591.

Niwa, Y., Kanda, G. N., Yamada, R. G., Shi, S., Sunagawa, G. A., Ukai-Tadenuma, M., ... & Ueda, H. R. (2018). Muscarinic acetylcholine receptors Chrm1 and Chrm3 are essential for REM sleep. Cell Reports, 24(9), 2231-2247.

Deng, A. Y., deBlois, D., Laporte, S. A., Gelinas, D., Tardif, J. C., Thorin, E., ... & Ménard, A. (2018). Novel pathogenesis of hypertension and diastolic dysfunction caused by M3R (muscarinic cholinergic 3 receptor) signaling. Hypertension, 72(3), 755-764.

Patel, K. R., Bai, Y., Trieu, K. G., Barrios, J., & Ai, X. (2017). Targeting acetylcholine receptor M3 prevents the progression of airway hyperreactivity in a mouse model of childhood asthma. The FASEB Journal, 31(10), 4335-4346.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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