Mouse Anti-CHRNA10 Recombinant Antibody (CBT2008) (V2LY-0625-LY814)

Name: Mouse Anti-CHRNA10 Recombinant Antibody (CBT2008)
SKU: V2LY-0625-LY814
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT2008

Antibody Isotype

IgG2a

Application

Basic Information

Immunogen

Purified recombinant fragment of human CHRNA10 (AA: extra 25-237) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Cholinergic Receptor Nicotinic Alpha 10 Subunit

Entrez Gene ID

57053

UniProt ID

Q9GZZ6

Function

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.

Biological Process

Chemical synaptic transmission Source: GO_Central
Detection of mechanical stimulus involved in sensory perception of sound Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Ion transmembrane transport Source: GO_Central
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Nervous system process Source: GO_Central
Positive regulation of cytosolic calcium ion concentration Source: MGI
Regulation of cell population proliferation Source: UniProtKB
Regulation of membrane potential Source: GO_Central
Signal transduction Source: GO_Central
Synaptic transmission, cholinergic Source: UniProtKB

Cellular Location

Postsynaptic cell membrane; Cell membrane

Topology

Extracellular: 25-237
Helical: 238-258
Helical: 268-288
Helical: 302-322
Cytoplasmic: 323-428
Helical: 429-449

More Infomation

References

AlSharari, S. D., Toma, W., Mahmood, H. M., McIntosh, J. M., & Damaj, M. I. (2020). The α9α10 nicotinic acetylcholine receptors antagonist α-conotoxin RgIA reverses colitis signs in murine dextran sodium sulfate model. European Journal of Pharmacology, 883, 173320.

Zouridakis, M., Papakyriakou, A., Ivanov, I. A., Kasheverov, I. E., Tsetlin, V., Tzartos, S., & Giastas, P. (2019). Crystal structure of the monomeric extracellular domain of α9 nicotinic receptor subunit in complex with α-conotoxin RgIA: Molecular dynamics insights into RgIA binding to α9α10 nicotinic receptors. Frontiers in pharmacology, 10, 474.

Morley, B. J., Whiteaker, P., & Elgoyhen, A. B. (2018). Commentary: Nicotinic acetylcholine receptor α9 and α10 subunits are expressed in the brain of mice. Frontiers in cellular neuroscience, 12, 104.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CHRNA10 Recombinant Antibody (CBT2966)

Mouse Anti-CHRNA10 Recombinant Antibody (EG685)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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