Rabbit Anti-CISD1 Recombinant Antibody (CBWJZ-058) (CBMAB-Z0127-WJ)

Name: Rabbit Anti-CISD1 Recombinant Antibody (CBWJZ-058)
SKU: CBMAB-Z0127-WJ
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-CISD1 Recombinant Antibody (CBWJZ-058) (CBMAB-Z0127-WJ) in IHC

The expression of CISD1 in LUAD tissue was examined by IHC assay. ...View More

Jiang, X., Yuan, Y., Tang, L., Wang, J., Zhang, D., & Duan, L. (2022). Systematic analysis and validation of the prognosis, immunological role and biology function of the ferroptosis-related lncRNA GSEC/miRNA-101-3p/CISD1 axis in lung adenocarcinoma. Frontiers in Molecular Biosciences, 8, 793732.

Distributed under Open Access license CC BY 4.0, without modification.

Rabbit Anti-CISD1 Recombinant Antibody (CBWJZ-058) (CBMAB-Z0127-WJ) in WB

Representative Western blots for OXPHOS, from heart or skeletal muscle tissue (n = 5), with quantitation shown in the graph for complex I-V. Representative Western blots for TOM20, n = 4; mitoNEET, n = 4; tDRP1, n = 4; OPA1, n = 3; Ferritin, n = 4; mitoFerritin, n = 4 in both th3/+ and WT groups, with quantitative analysis shown in graphs as mean ± SEM. ...View More

Reboucas, P., Fillebeen, C., Botta, A., Cleverdon, R., Steele, A. P., Richard, V., ... & Sweeney, G. (2023). Discovery-Based Proteomics Identify Skeletal Muscle Mitochondrial Alterations as an Early Metabolic Defect in a Mouse Model of β-Thalassemia. International Journal of Molecular Sciences, 24(5), 4402.

Distributed under Open Access license CC BY 4.0, without modification.

Rabbit Anti-CISD1 Recombinant Antibody (CBWJZ-058) (CBMAB-Z0127-WJ) in WB

Western blotting for MitoNEET in EV and MitoN cells showed expression of the exogenous flagged-tagged MitoNEET (13 kDa) relative to the endogenous version (12 kDa). ...View More

Tam, E., Sung, H. K., Lam, N. H., You, S., Cho, S., Ahmed, S. M., ... & Sweeney, G. (2022). Role of mitochondrial iron overload in mediating cell death in H9c2 cells. Cells, 12(1), 118.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

CBWJZ-058

Antibody Isotype

IgG

Application

WB, IP

Basic Information

Immunogen

Synthetic peptide corresponding to residues surrounding Asp61 of human CISD1/mitoNEET protein.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100ug/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Target

Full Name

CDGSH Iron Sulfur Domain 1

Introduction

CISD1 (CDGSH Iron Sulfur Domain 1) is a Protein Coding gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. Diseases associated with CISD1 include Wolfram Syndrome. Among its related pathways are Adipogenesis and Glucose / Energy Metabolism.

Entrez Gene ID

Human	55847
Mouse	52637
Rat	294362

UniProt ID

Human	Q9NZ45
Mouse	Q91WS0
Rat	B0K020

Alternative Names

CD1; MDS029; C10orf70; mitoNEET

Function

Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity).

May be involved in Fe-S cluster shuttling and/or in redox reactions.

Biological Process

Regulation of cellular respiration Source: Ensembl

Cellular Location

Mitochondrion outer membrane

Topology

Helical: 14-31
Cytoplasmic: 32-108

PTM

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

More Infomation

References

Hua, J., Gao, Z., Zhong, S., Wei, B., Zhu, J., & Ying, R. (2021). CISD1 protects against atherosclerosis by suppressing lipid accumulation and inflammation via mediating Drp1. Biochemical and Biophysical Research Communications, 577, 80-88.

Hsiung, K. C., Liu, K. Y., Tsai, T. F., Yoshina, S., Mitani, S., Tan, B. C. M., & Lo, S. J. (2020). Defects in CISD-1, a mitochondrial iron-sulfur protein, lower glucose level and ATP production in Caenorhabditis elegans. Biomedical journal, 43(1), 32-43.

Sengupta, S., Nechushtai, R., Jennings, P. A., Jose’N, O., Padilla, P. A., Azad, R. K., & Mittler, R. (2018). Phylogenetic analysis of the CDGSH iron-sulfur binding domain reveals its ancient origin. Scientific reports, 8(1), 1-12.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CISD1 Recombinant Antibody (3A1)

Mouse Anti-CISD1 Recombinant Antibody (2B3)

Mouse Anti-CISD1 Recombinant Antibody (1A8)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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