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Rabbit Anti-CISH Recombinant Antibody (CBWJC-2722) (CBMAB-C3818WJ)

This product is a Rabbit antibody that recognizes CISH. This antibody CBWJC-2722 can be used for immunoassay techniques such as: WB, IP.
See all CISH antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBWJC-2722
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytokine Inducible SH2 Containing Protein
Introduction
The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Entrez Gene ID
1154
UniProt ID
Q9NSE2
Alternative Names
Cytokine Inducible SH2 Containing Protein; Suppressor Of Cytokine Signaling; CIS-1; SOCS; CIS; G18;
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Biological Process
Interleukin-7-mediated signaling pathway Source: Reactome
Intracellular signal transduction Source: UniProtKB
Negative regulation of signal transduction Source: UniProtKB-KW
Phosphatidylinositol phosphorylation Source: GO_Central
Post-translational protein modification Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: Ensembl
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of cell growth Source: UniProtKB
Cellular Location
Cytosol; Plasma membrane; Phosphatidylinositol 3-kinase complex
PTM
Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).

Kotas, M. E., Mroz, N. M., Koga, S., Liang, H. E., Schroeder, A. W., Ricardo-Gonzalez, R. R., ... & Locksley, R. M. (2021). CISH constrains the tuft–ILC2 circuit to set epithelial and immune tone. Mucosal immunology, 14(6), 1295-1305.

Ruan, Z., Chen, G., Xie, T., Mo, G., Wang, G., Luo, W., ... & Zhang, X. (2021). Cytokine inducible SH2-containing protein potentiate J subgroup avian leukosis virus replication and suppress antiviral responses in DF-1 chicken fibroblast cells. Virus Research, 296, 198344.

Chen, G., Chen, J., Wu, J., Ren, X., Li, L., Lu, S., ... & Luo, W. (2021). Integrative Analyses of mRNA Expression Profile Reveal SOCS2 and CISH Play Important Roles in GHR Mutation-Induced Excessive Abdominal Fat Deposition in the Sex-Linked Dwarf Chicken. Frontiers in genetics, 1667.

Zhu, H., Blum, R. H., Bernareggi, D., Ask, E. H., Wu, Z., Hoel, H. J., ... & Kaufman, D. S. (2020). Metabolic reprograming via deletion of CISH in human iPSC-derived NK cells promotes in vivo persistence and enhances anti-tumor activity. Cell Stem Cell, 27(2), 224-237.

Peng, H. Y., Hsiao, J. R., Chou, S. T., Hsu, Y. M., Wu, G. H., Shieh, Y. S., & Shiah, S. G. (2020). MiR-944/CISH mediated inflammation via STAT3 is involved in oral cancer malignance by cigarette smoking. Neoplasia, 22(11), 554-565.

Niu, B., Guo, D., Liu, Z., Han, X., & Wang, X. (2017). Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits. Asian-Australasian journal of animal sciences, 30(12), 1689.

Queval, C. J., Song, O. R., Carralot, J. P., Saliou, J. M., Bongiovanni, A., Deloison, G., ... & Brodin, P. (2017). Mycobacterium tuberculosis controls phagosomal acidification by targeting CISH-mediated signaling. Cell reports, 20(13), 3188-3198.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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