Rat Anti-CISH Recombinant Antibody (C7738) (CBMAB-C7738-LY)

Name: Rat Anti-CISH Recombinant Antibody (C7738)
SKU: CBMAB-C7738-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

C7738

Application

Immunogen

Recombinant human CIS-1 Leu11-Leu258.

Host Species

Rat

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

0.5 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytokine Inducible SH2 Containing Protein

Introduction

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Entrez Gene ID

1154

UniProt ID

Q9NSE2

Alternative Names

Cytokine Inducible SH2 Containing Protein; Suppressor Of Cytokine Signaling; CIS-1; SOCS; CIS; G18;

Function

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).

Biological Process

Interleukin-7-mediated signaling pathway Source: Reactome
Intracellular signal transduction Source: UniProtKB
Negative regulation of signal transduction Source: UniProtKB-KW
Phosphatidylinositol phosphorylation Source: GO_Central
Post-translational protein modification Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: Ensembl
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of cell growth Source: UniProtKB

Cellular Location

Cytosol; Plasma membrane; Phosphatidylinositol 3-kinase complex

PTM

Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).

Kotas, M. E., Mroz, N. M., Koga, S., Liang, H. E., Schroeder, A. W., Ricardo-Gonzalez, R. R., ... & Locksley, R. M. (2021). CISH constrains the tuft–ILC2 circuit to set epithelial and immune tone. Mucosal immunology, 14(6), 1295-1305.

Ruan, Z., Chen, G., Xie, T., Mo, G., Wang, G., Luo, W., ... & Zhang, X. (2021). Cytokine inducible SH2-containing protein potentiate J subgroup avian leukosis virus replication and suppress antiviral responses in DF-1 chicken fibroblast cells. Virus Research, 296, 198344.

Chen, G., Chen, J., Wu, J., Ren, X., Li, L., Lu, S., ... & Luo, W. (2021). Integrative Analyses of mRNA Expression Profile Reveal SOCS2 and CISH Play Important Roles in GHR Mutation-Induced Excessive Abdominal Fat Deposition in the Sex-Linked Dwarf Chicken. Frontiers in genetics, 1667.

Zhu, H., Blum, R. H., Bernareggi, D., Ask, E. H., Wu, Z., Hoel, H. J., ... & Kaufman, D. S. (2020). Metabolic reprograming via deletion of CISH in human iPSC-derived NK cells promotes in vivo persistence and enhances anti-tumor activity. Cell Stem Cell, 27(2), 224-237.

Peng, H. Y., Hsiao, J. R., Chou, S. T., Hsu, Y. M., Wu, G. H., Shieh, Y. S., & Shiah, S. G. (2020). MiR-944/CISH mediated inflammation via STAT3 is involved in oral cancer malignance by cigarette smoking. Neoplasia, 22(11), 554-565.

Niu, B., Guo, D., Liu, Z., Han, X., & Wang, X. (2017). Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits. Asian-Australasian journal of animal sciences, 30(12), 1689.

Queval, C. J., Song, O. R., Carralot, J. P., Saliou, J. M., Bongiovanni, A., Deloison, G., ... & Brodin, P. (2017). Mycobacterium tuberculosis controls phagosomal acidification by targeting CISH-mediated signaling. Cell reports, 20(13), 3188-3198.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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