Rabbit Anti-CLDN18 Recombinant Antibody (C5556) (CBMAB-C5556-LY)

Name: Rabbit Anti-CLDN18 Recombinant Antibody (C5556)
SKU: CBMAB-C5556-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

C5556

Application

IHC-P

Immunogen

A synthetic peptide corresponding to the N-terminus human Claudin 18.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	1:5,000-1:20,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Claudin 18

Introduction

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]

Entrez Gene ID

51208

UniProt ID

P56856

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Biological Process

Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Digestive tract development Source: Ensembl
Negative regulation of bone resorption Source: Ensembl
Negative regulation of osteoclast development Source: Ensembl
Negative regulation of protein localization to nucleus Source: Ensembl
Response to ethanol Source: Ensembl
TNFSF11-mediated signaling pathway Source: Ensembl

Cellular Location

Cell membrane; Tight junction. Localizes to tight junctions in epithelial cells.

Topology

Cytoplasmic: 1-6
Helical: 7-27
Extracellular: 28-80
Helical: 81-101
Cytoplasmic: 102-122
Helical: 123-143
Extracellular: 144-174
Helical: 175-195
Cytoplasmic: 196-261

Iwaya, M., Hayashi, H., Nakajima, T., Matsuda, K., Kinugawa, Y., Tobe, Y., ... & Ota, H. (2021). Colitis‐associated colorectal adenocarcinomas frequently express claudin 18 isoform 2: implications for claudin 18.2 monoclonal antibody therapy. Histopathology, 79(2), 227-237.

Caron, T. J., Scott, K. E., Sinha, N., Muthupalani, S., Baqai, M., Ang, L. H., ... & Hagen, S. J. (2021). Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells. Cellular and Molecular Gastroenterology and Hepatology, 11(3), 783-801.

Sato, K., Matsumoto, I., Suzuki, K., Tamura, A., Shiraishi, A., Kiyonari, H., ... & Kawakami, K. (2021). Deficiency of lung-specific claudin-18 leads to aggravated infection with Cryptococcus deneoformans through dysregulation of the microenvironment in lungs. Scientific reports, 11(1), 1-13.

Lee, P. H., Hong, J., & Jang, A. S. (2020). N-acetylcysteine decreases airway inflammation and responsiveness in asthma by modulating claudin 18 expression. The Korean journal of internal medicine, 35(5), 1229.

Coati, I., Lotz, G., Fanelli, G. N., Brignola, S., Lanza, C., Cappellesso, R., ... & Fassan, M. (2019). Claudin-18 expression in oesophagogastric adenocarcinomas: a tissue microarray study of 523 molecularly profiled cases. British journal of cancer, 121(3), 257-263.

Hagen, S. J., Ang, L. H., Zheng, Y., Karahan, S. N., Wu, J., Wang, Y. E., ... & Fox, J. G. (2018). Loss of tight junction protein claudin 18 promotes progressive neoplasia development in mouse stomach. Gastroenterology, 155(6), 1852-1867.

Zhou, B., Flodby, P., Luo, J., Castillo, D. R., Liu, Y., Yu, F. X., ... & Borok, Z. (2018). Claudin-18–mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. The Journal of clinical investigation, 128(3), 970-984.

Kotton, D. N. (2018). Claudin-18: unexpected regulator of lung alveolar epithelial cell proliferation. The Journal of clinical investigation, 128(3), 903-905.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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