Rat Anti-CLEC10A Recombinant Antibody (CBFYC-1911) (V2LY-1206-LY74)

Name: Rat Anti-CLEC10A Recombinant Antibody (CBFYC-1911)
SKU: V2LY-1206-LY74
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBFYC-1911

Application

WB, IP, IF, FC

Immunogen

Macrophage cell lines of mouse.

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-type lectin domain family 10, member A

Entrez Gene ID

17312

UniProt ID

P49300

Function

Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Endocytosis Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Stimulatory C-type lectin receptor signaling pathway Source: Reactome

Cellular Location

Membrane

Topology

Cytoplasmic: 1-39
Helical: 40-60
Extracellular: 61-316

Zou, M., Wu, H., Zhou, M., Xiao, F., Abudushalamu, G., Yao, Y., ... & Wu, G. (2021). High expression of CLEC10A in head and neck squamous cell carcinoma indicates favorable prognosis and high‐level immune infiltration status. Cellular Immunology, 104472.

Eggink, L. L., Roby, K. F., Cote, R., & Hoober, J. K. (2018). An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides. Journal for immunotherapy of cancer, 6(1), 1-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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