Mouse Anti-CLEC4A Recombinant Antibody (9E8) (V2LY-1206-LY133)

Name: Mouse Anti-CLEC4A Recombinant Antibody (9E8)
SKU: V2LY-1206-LY133
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

9E8

Application

Immunogen

BALB/c mice with a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-Type Lectin Domain Family 4 Member A

Entrez Gene ID

50856

UniProt ID

Q9UMR7

Function

C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca2+-dependent manner (PubMed:27015765).

Involved in regulating immune reactivity (PubMed:18258799, PubMed:10438934).

Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8+ T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8+ T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286).

In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799).

May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934).

(Microbial infection) Involved in the interaction between HIV-1 virus and dendritic cells. Enhances HIV-1 binding/entry and virus infection. Requires ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases and MAP kinases.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation of exogenous peptide antigen via MHC class I Source: UniProtKB
CD8-positive, alpha-beta T cell activation Source: UniProtKB
Cell adhesion Source: ProtInc
Cell surface receptor signaling pathway Source: ProtInc
Innate immune response Source: UniProtKB-KW
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: UniProtKB
Plasmacytoid dendritic cell antigen processing and presentation Source: UniProtKB
Stimulatory C-type lectin receptor signaling pathway Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-48
Helical: 49-69
Extracellular: 70-237

Park, I., Goddard, M. E., Cole, J. E., Zanin, N., Lyytikäinen, L. P., Lehtimäki, T., ... & Monaco, C. (2022). C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis. Nature Communications, 13(1), 1-17.

Álvarez, B., Revilla, C., Domínguez, J., & Ezquerra, A. (2021). Expression of CLEC4A in porcine tissues and leukocyte populations and characterization of mRNA splice variants. Molecular Immunology, 132, 157-164.

Nasu, J., Uto, T., Fukaya, T., Takagi, H., Fukui, T., Miyanaga, N., ... & Sato, K. (2020). Pivotal role of the carbohydrate recognition domain in self-interaction of CLEC4A to elicit the ITIM-mediated inhibitory function in murine conventional dendritic cells in vitro. International immunology, 32(10), 673-682.

Weng, T. Y., Li, C. J., Li, C. Y., Hung, Y. H., Yen, M. C., Chang, Y. W., ... & Lai, M. D. (2017). Skin delivery of Clec4a small hairpin RNA elicited an effective antitumor response by enhancing CD8+ immunity in vivo. Molecular Therapy-Nucleic Acids, 9, 419-427.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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