Human Recombinant CLEC4A protein, ECD, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY3580)

Name: Human Recombinant CLEC4A protein, ECD, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY3580
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & AVI Tag

Protein Construction

This product is Human Recombinant CLEC4A protein, ECD, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 70-237 and predicts a molecular mass of 23.05 kDa.

Molecule Mass

23.05 kDa

Protein Domain

ECD

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 70-237

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

C-Type Lectin Domain Family 4 Member A

Function

C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca2+-dependent manner (PubMed:27015765).

Involved in regulating immune reactivity (PubMed:18258799, PubMed:10438934).

Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8+ T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8+ T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286).

In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799).

May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934).

(Microbial infection) Involved in the interaction between HIV-1 virus and dendritic cells. Enhances HIV-1 binding/entry and virus infection. Requires ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases and MAP kinases.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation of exogenous peptide antigen via MHC class I Source: UniProtKB
CD8-positive, alpha-beta T cell activation Source: UniProtKB
Cell adhesion Source: ProtInc
Cell surface receptor signaling pathway Source: ProtInc
Innate immune response Source: UniProtKB-KW
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: UniProtKB
Plasmacytoid dendritic cell antigen processing and presentation Source: UniProtKB
Stimulatory C-type lectin receptor signaling pathway Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-48
Helical: 49-69
Extracellular: 70-237

Park, I., Goddard, M. E., Cole, J. E., Zanin, N., Lyytikäinen, L. P., Lehtimäki, T., ... & Monaco, C. (2022). C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis. Nature Communications, 13(1), 1-17.

Álvarez, B., Revilla, C., Domínguez, J., & Ezquerra, A. (2021). Expression of CLEC4A in porcine tissues and leukocyte populations and characterization of mRNA splice variants. Molecular Immunology, 132, 157-164.

Nasu, J., Uto, T., Fukaya, T., Takagi, H., Fukui, T., Miyanaga, N., ... & Sato, K. (2020). Pivotal role of the carbohydrate recognition domain in self-interaction of CLEC4A to elicit the ITIM-mediated inhibitory function in murine conventional dendritic cells in vitro. International immunology, 32(10), 673-682.

Weng, T. Y., Li, C. J., Li, C. Y., Hung, Y. H., Yen, M. C., Chang, Y. W., ... & Lai, M. D. (2017). Skin delivery of Clec4a small hairpin RNA elicited an effective antitumor response by enhancing CD8+ immunity in vivo. Molecular Therapy-Nucleic Acids, 9, 419-427.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CLEC4A protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3579)

Human Recombinant CLEC4A protein, His Tag (CAT#: V2LY-0526-LY3581)

Cynomolgus Recombinant CLEC4A protein, His Tag (CAT#: V2LY-0526-LY1481)

Mouse Recombinant CLEC4A protein, ECD, His Tag (CAT#: V2LY-0526-LY8198)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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