Mouse Anti-CLEC4A Recombinant Antibody (CBYCD-129) (CBMAB-D0086-YC)

Name: Mouse Anti-CLEC4A Recombinant Antibody (CBYCD-129)
SKU: CBMAB-D0086-YC
Rating: 5 (1 reviews)

See all CLEC4A antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-129

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 191-237 mapping within a C-terminal extracellular domain of DCIR of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

C-Type Lectin Domain Family 4 Member A

Introduction

CLEC4A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in inflammatory and immune response.

Entrez Gene ID

Human	50856
Mouse	53624
Rat	474143

UniProt ID

Human	Q9UMR7
Mouse	Q9QZ15
Rat	Q67EQ0

Alternative Names

C-Type Lectin Domain Family 4 Member A; C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 6; C-Type Lectin Superfamily Member 6; Dendritic Cell Immunoreceptor; Lectin-Like Immunoreceptor; C-Type Lectin DDB27; CLECSF6;

Function

C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca2+-dependent manner (PubMed:27015765).

Involved in regulating immune reactivity (PubMed:18258799, PubMed:10438934).

Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8+ T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8+ T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13 (PubMed:18258799, PubMed:20530286).

In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (PubMed:18258799).

May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:10438934).

(Microbial infection) Involved in the interaction between HIV-1 virus and dendritic cells. Enhances HIV-1 binding/entry and virus infection. Requires ITIM motif-associated signal transduction pathway involving phosphatases PTPN6 and PTPN11, SYK, Src kinases and MAP kinases.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation of exogenous peptide antigen via MHC class I Source: UniProtKB
CD8-positive, alpha-beta T cell activation Source: UniProtKB
Cell adhesion Source: ProtInc
Cell surface receptor signaling pathway Source: ProtInc
Innate immune response Source: UniProtKB-KW
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: UniProtKB
Plasmacytoid dendritic cell antigen processing and presentation Source: UniProtKB
Stimulatory C-type lectin receptor signaling pathway Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-48
Helical: 49-69
Extracellular: 70-237

Park, I., Goddard, M. E., Cole, J. E., Zanin, N., Lyytikäinen, L. P., Lehtimäki, T., ... & Monaco, C. (2022). C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis. Nature Communications, 13(1), 1-17.

Álvarez, B., Revilla, C., Domínguez, J., & Ezquerra, A. (2021). Expression of CLEC4A in porcine tissues and leukocyte populations and characterization of mRNA splice variants. Molecular Immunology, 132, 157-164.

Nasu, J., Uto, T., Fukaya, T., Takagi, H., Fukui, T., Miyanaga, N., ... & Sato, K. (2020). Pivotal role of the carbohydrate recognition domain in self-interaction of CLEC4A to elicit the ITIM-mediated inhibitory function in murine conventional dendritic cells in vitro. International immunology, 32(10), 673-682.

Weng, T. Y., Li, C. J., Li, C. Y., Hung, Y. H., Yen, M. C., Chang, Y. W., ... & Lai, M. D. (2017). Skin delivery of Clec4a small hairpin RNA elicited an effective antitumor response by enhancing CD8+ immunity in vivo. Molecular Therapy-Nucleic Acids, 9, 419-427.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CLEC4A Recombinant Antibody (CBYCD-129)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CLEC4A Recombinant Antibody (D0085) (CAT#: V2LY-1206-LY125)

Rat Anti-CLEC4A Recombinant Antibody (C0033) (CAT#: V2LY-1206-LY129)

Mouse Anti-CLEC4A Recombinant Antibody (CBXC-0465) (CAT#: V2LY-1206-LY132)

Mouse Recombinant CLEC4A protein, ECD, His Tag (CAT#: V2LY-0526-LY8198)

Human Recombinant CLEC4A protein, His Tag (CAT#: V2LY-0526-LY3581)

Cynomolgus Recombinant CLEC4A protein, His Tag (CAT#: V2LY-0526-LY1481)

Mouse Anti-CLEC4A Recombinant Antibody (9E8) (CAT#: V2LY-1206-LY133)

Mouse Anti-CLEC4A Recombinant Antibody (D0083) (CAT#: V2LY-1206-LY123)

Mouse Anti-CLEC4A Recombinant Antibody (CBT4394) (CAT#: V2LY-0625-LY1031)

Mouse Anti-CLEC4A Recombinant Antibody (5P20) (CAT#: V2LY-1206-LY122)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry