Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987) (V2LY-1206-LY430)

Name: Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987)
SKU: V2LY-1206-LY430
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987) (V2LY-1206-LY430) in ICC

Immunocytochemical analysis of smooth muscle (CALPONIN1, SM22-α, and α-SMA) markers with Hoechst 33342 nuclear counterstaining. ...View More

Koh, H., Kang, W., Mao, Y. Y., Park, J., Kim, S., Hong, S. H., & Lee, J. H. (2024). Employment of diverse in vitro systems for analyzing multiple aspects of disease, hereditary hemorrhagic telangiectasia (HHT). Cell & Bioscience, 14(1), 65.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987) (V2LY-1206-LY430) in Immunoblot

Lysates collected from HCT116 cells cultured under hypoxia and from tumor tissues were subjected to immunoblotting analysis. ...View More

Zheng, K., Sha, N., Hou, G., Leng, Z., Zhao, Q., Zhang, L., ... & Chen, T. (2023). IGF1R-phosphorylated PYCR1 facilitates ELK4 transcriptional activity and sustains tumor growth under hypoxia. Nature Communications, 14(1), 6117.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987) (V2LY-1206-LY430) in IHC

(A) Decalcified immunohistochemistry (IHC) staining of Calponin-1 expression at low and high magnification, outside and within the scaffold. Scale bars on low and high magnification represent 1 mm and 400 µm, respectively. (B) Quantification of Calponin-1 staining after different implantation times. Bars represent means ± std. dev. (n = 3). ...View More

Ouhaddi, Y., Charbonnier, B., Porge, J., Zhang, Y. L., Garcia, I., Gbureck, U., ... & Barralet, J. (2023). Development of neovasculature in axially vascularized calcium phosphate cement scaffolds. Journal of Functional Biomaterials, 14(2), 105.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987) (V2LY-1206-LY430) in WB

Western blot analysis showing the expression of Calponin 1, in EMCs stimulated with 0.5, 1, and 10 ng/mL of TGF β1 for 48, 72, and 96 h compared to control conditions (CTRL). ...View More

Pontemezzo, E., Foglio, E., Vernucci, E., Magenta, A., D'Agostino, M., Sileno, S., ... & Limana, F. (2021). miR-200c-3p regulates epitelial-to-mesenchymal transition in epicardial mesothelial cells by targeting epicardial follistatin-related protein 1. International Journal of Molecular Sciences, 22(9), 4971.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1987

Application

WB, IP, IF, IHC-P

Immunogen

Crude uterus extract of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Calponin 1

Entrez Gene ID

Human	1264
Mouse	12797
Rat	65204

UniProt ID

Human	P51911
Mouse	Q08091
Rat	Q08290

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Biological Process

Actomyosin structure organization Source: InterPro
Negative regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Regulation of smooth muscle contraction Source: UniProtKB

Cellular Location

Cytoskeleton; Focal adhesion

Choo, Y. Y., Sakai, T., Komatsu, S., Ikebe, R., Jeffers, A., Singh, K. P., ... & Ikebe, M. (2022). Calponin 1 contributes to myofibroblast differentiation of human pleural mesothelial cells. American Journal of Physiology-Lung Cellular and Molecular Physiology.

Mamoor, S. (2021). Differential expression of calponin 1 in cancers of the breast.

Liu, W., Fu, X., & Li, R. (2021). CNN1 regulates the DKK1/Wnt/β‑catenin/c‑myc signaling pathway by activating TIMP2 to inhibit the invasion, migration and EMT of lung squamous cell carcinoma cells. Experimental and therapeutic medicine, 22(2), 1-9.

Wang, Z., Li, T. E., Chen, M., Pan, J. J., & Shen, K. W. (2020). miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway. Aging (Albany NY), 12(2), 1867.

Feng, H. Z., Wang, H., Takahashi, K., & Jin, J. P. (2019). Double deletion of calponin 1 and calponin 2 in mice decreases systemic blood pressure with blunted length-tension response of aortic smooth muscle. Journal of molecular and cellular cardiology, 129, 49-57.

Zhao, H., Yang, K., Liu, Q., Hu, J., Wu, W., & Wang, J. (2019). Expression of calponin-1 and its pathogenic role in systemic sclerosis. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 39(3), 279-285.

Cetin, O., Karaman, E., Boza, B., Cim, N., & Sahin, H. G. (2018). Maternal serum calponin 1 level as a biomarker for the short-term prediction of preterm birth in women with threatened preterm labor. The Journal of Maternal-Fetal & Neonatal Medicine, 31(2), 216-222.

Xu, H., Wu, F., Zhang, H., Yang, C., Li, K., Wang, H., ... & Dai, Z. (2017). Actin cytoskeleton mediates BMP2-Smad signaling via calponin 1 in preosteoblast under simulated microgravity. Biochimie, 138, 184-193.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CNN1 Matched Antibody Pair (282) (CAT#: APMAB-282LY)

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Isotype control

For research use only. Not intended for any clinical use.

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